中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2014年
5期
424-427
,共4页
王敬萍%马秀瑞%张月安%王慧仙%杨晋静%董晋%王建玲%杨燕%李保
王敬萍%馬秀瑞%張月安%王慧仙%楊晉靜%董晉%王建玲%楊燕%李保
왕경평%마수서%장월안%왕혜선%양진정%동진%왕건령%양연%리보
心力衰竭,充血性%受体,肾上腺素能β3%抗体
心力衰竭,充血性%受體,腎上腺素能β3%抗體
심력쇠갈,충혈성%수체,신상선소능β3%항체
Heart failure,congestive%Receptors,adrenergic beta-3%Antibodies
目的 探讨抗β3肾上腺素能受体抗体(β3AA)对心力衰竭大鼠心脏是否具有保护作用.方法 本研究采用雄性Wistar大鼠为研究对象.合成β3肾上腺素能受体抗原肽段,将该抗原肽段主动免疫大鼠,经ELISA方法检测免疫鼠血清中β3AA水平,纯化β3AA阳性的免疫大鼠血清中的免疫球蛋白IgG.将大鼠分为假手术组(n=8)、心力衰竭组(HF组,n=8)、β3AA被动免疫组(HF+β3AA组,n=8)和β3AA被动免疫阴性对照组(HF+ IgG组,n=8).采用腹主动脉缩窄术建立心力衰竭大鼠模型.术后8周应用ELISA法检测大鼠血清N末端B型利钠肽原(NT-proBNP)表达水平,并应用超声心动图检测各组大鼠心功能.结果 β3AA免疫后50 d,血清中β3AA维持稳定.大鼠腹主动脉缩窄术后8周,HF组大鼠左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)、室间隔厚度(IVS)均明显大于假手术组[分别为(6.92 ±0.22) mm比(5.62±0.19) mm(P ＜0.001),(4.63±0.23) mm比(3.50±0.20) mm(P＜0.01)和(2.44±0.06) mm比(2.28 ±0.05) mm(P＜0.05)],左心室射血分数(LVEF)、左心室短轴缩短率(LVFS)则明显低于假手术组[分别为(62.07±3.99)％比(79.63±3.02)％(P＜0.01)和(31.46±3.22)％比(43.65±2.68)％ (P ＜0.05)].术后8周,HF+β3AA组大鼠LVEDD和LVESD均显著低于HF+ IgG组[(6.07 ±0.30) mm比(6.92±0.24) mm(P ＜0.05)和(3.92 ±0.22) mm比(4.68 ±0.23)mm(P＜0.05)],LVEF和LVFS则显著高于HF+ IgG组[(70.29±1.78)％比(61.95±3.03)％(P＜0.05)和(38.08±2.32)％比(30.50±1.82)％ (P＜0.05)].HF+β3AA组大鼠血清NT-proBNP水平明显低于HF+ IgG组[(196.43±6.56) pg/ml 比(242.13±7.86) pg/ml,P＜0.01].结论 β3AA被动免疫心力衰竭大鼠后可以改善心功能,并降低血清NT-proBNP水平,提示β3AA对压力后负荷性心力衰竭大鼠具有心脏保护作用.
目的 探討抗β3腎上腺素能受體抗體(β3AA)對心力衰竭大鼠心髒是否具有保護作用.方法 本研究採用雄性Wistar大鼠為研究對象.閤成β3腎上腺素能受體抗原肽段,將該抗原肽段主動免疫大鼠,經ELISA方法檢測免疫鼠血清中β3AA水平,純化β3AA暘性的免疫大鼠血清中的免疫毬蛋白IgG.將大鼠分為假手術組(n=8)、心力衰竭組(HF組,n=8)、β3AA被動免疫組(HF+β3AA組,n=8)和β3AA被動免疫陰性對照組(HF+ IgG組,n=8).採用腹主動脈縮窄術建立心力衰竭大鼠模型.術後8週應用ELISA法檢測大鼠血清N末耑B型利鈉肽原(NT-proBNP)錶達水平,併應用超聲心動圖檢測各組大鼠心功能.結果 β3AA免疫後50 d,血清中β3AA維持穩定.大鼠腹主動脈縮窄術後8週,HF組大鼠左心室舒張末期內徑(LVEDD)、左心室收縮末期內徑(LVESD)、室間隔厚度(IVS)均明顯大于假手術組[分彆為(6.92 ±0.22) mm比(5.62±0.19) mm(P ＜0.001),(4.63±0.23) mm比(3.50±0.20) mm(P＜0.01)和(2.44±0.06) mm比(2.28 ±0.05) mm(P＜0.05)],左心室射血分數(LVEF)、左心室短軸縮短率(LVFS)則明顯低于假手術組[分彆為(62.07±3.99)％比(79.63±3.02)％(P＜0.01)和(31.46±3.22)％比(43.65±2.68)％ (P ＜0.05)].術後8週,HF+β3AA組大鼠LVEDD和LVESD均顯著低于HF+ IgG組[(6.07 ±0.30) mm比(6.92±0.24) mm(P ＜0.05)和(3.92 ±0.22) mm比(4.68 ±0.23)mm(P＜0.05)],LVEF和LVFS則顯著高于HF+ IgG組[(70.29±1.78)％比(61.95±3.03)％(P＜0.05)和(38.08±2.32)％比(30.50±1.82)％ (P＜0.05)].HF+β3AA組大鼠血清NT-proBNP水平明顯低于HF+ IgG組[(196.43±6.56) pg/ml 比(242.13±7.86) pg/ml,P＜0.01].結論 β3AA被動免疫心力衰竭大鼠後可以改善心功能,併降低血清NT-proBNP水平,提示β3AA對壓力後負荷性心力衰竭大鼠具有心髒保護作用.
목적 탐토항β3신상선소능수체항체(β3AA)대심력쇠갈대서심장시부구유보호작용.방법 본연구채용웅성Wistar대서위연구대상.합성β3신상선소능수체항원태단,장해항원태단주동면역대서,경ELISA방법검측면역서혈청중β3AA수평,순화β3AA양성적면역대서혈청중적면역구단백IgG.장대서분위가수술조(n=8)、심력쇠갈조(HF조,n=8)、β3AA피동면역조(HF+β3AA조,n=8)화β3AA피동면역음성대조조(HF+ IgG조,n=8).채용복주동맥축착술건립심력쇠갈대서모형.술후8주응용ELISA법검측대서혈청N말단B형리납태원(NT-proBNP)표체수평,병응용초성심동도검측각조대서심공능.결과 β3AA면역후50 d,혈청중β3AA유지은정.대서복주동맥축착술후8주,HF조대서좌심실서장말기내경(LVEDD)、좌심실수축말기내경(LVESD)、실간격후도(IVS)균명현대우가수술조[분별위(6.92 ±0.22) mm비(5.62±0.19) mm(P ＜0.001),(4.63±0.23) mm비(3.50±0.20) mm(P＜0.01)화(2.44±0.06) mm비(2.28 ±0.05) mm(P＜0.05)],좌심실사혈분수(LVEF)、좌심실단축축단솔(LVFS)칙명현저우가수술조[분별위(62.07±3.99)％비(79.63±3.02)％(P＜0.01)화(31.46±3.22)％비(43.65±2.68)％ (P ＜0.05)].술후8주,HF+β3AA조대서LVEDD화LVESD균현저저우HF+ IgG조[(6.07 ±0.30) mm비(6.92±0.24) mm(P ＜0.05)화(3.92 ±0.22) mm비(4.68 ±0.23)mm(P＜0.05)],LVEF화LVFS칙현저고우HF+ IgG조[(70.29±1.78)％비(61.95±3.03)％(P＜0.05)화(38.08±2.32)％비(30.50±1.82)％ (P＜0.05)].HF+β3AA조대서혈청NT-proBNP수평명현저우HF+ IgG조[(196.43±6.56) pg/ml 비(242.13±7.86) pg/ml,P＜0.01].결론 β3AA피동면역심력쇠갈대서후가이개선심공능,병강저혈청NT-proBNP수평,제시β3AA대압력후부하성심력쇠갈대서구유심장보호작용.
Objective To explore the effect of the autoantibody against the β3-adrenoceptor on rats with experimental heart failure.Method The peptide corresponding to the sequence of β3 adrenoceptor was synthesized to actively immunize the rats,ELISA was used to detect the serum level of autoantibody against the β3-adrenoceptor (β3AA).Total IgGs were extracted from the serum containing β3AA in immunized rats.Aortic banding surgery was used to establish the heart failure model in male Wistar rats and rats were divided into the sham group (n =8),heart failure group (n =8),β3AA-immunized heart failure group (HF + β3AA,n =8) and corresponding negative IgG-immunized heart failure group (HF + IgG,n =8).In 6 weeks and 8 weeks after aortic banding surgery,the serum levels of NT-pro brain natriuretic peptide (NT-proBNP) were assayed with ELISA assay and cardiac function was assessed by echocardiography.Results β3AA was used to immunize rat with heart failure,the serum level of β3AA was stable at 50 days post immunization.At 8 weeks after aortic banding surgery,heart failure group showed significantly increased LVEDD [(6.92±0.22) mm vs.(5.62 ±0.19) mm,P＜0.001],LVESD [(4.63 ±0.23) mm vs.(3.50±0.20) mm,P＜0.01] and IVS[(2.44 ±0.06) mm vs.(2.28 ±0.05) mm,P ＜0.05],and decreased LVEF [(62.07 ± 3.99) ％ vs.(79.63 ± 3.02) ％,P ＜ 0.01] and LVFS [(31.46 ± 3.22) ％ vs.(43.65 ±2.68) ％,P＜0.05] compared with the sham group.HF + β3AA IgG group showed decreased LVEDD [(6.07±0.30) mm vs.(6.92 ±0.24) mm,P＜0.05] and LVESD[(3.92±0.22) mm vs.(4.68 ± 0.23) mm,P＜0.05],and higher LVEF [(70.29 ± 1.78) ％ vs.(61.95 ± 3.03) ％,P ＜ 0.05]and LVFS [(38.08 ± 2.32) ％ vs.(30.50 ± 1.82) ％,P＜0.05] compared to the HF + IgG group.In addition,compared with the HF + IgG group,HF + β3AA IgG group showed decreased serum levels of NT-proBNP [(196.43±6.56) pg/ml vs.(242.13 ±7.86) pg/ml,P＜0.01].Conclusion Our results demonstrate that β3AA can improve cardiac function and reduce the serum levels of NT-proBNP in rat with heart failure.
