CAJ | 학술논문

目的探讨非小细胞肺癌(NSCLC)中TCF21基因mRNA和蛋白的表达水平及其启动子区甲基化状态的意义.方法运用RT-PCR、蛋白印迹法(Western-blot)和焦磷酸测序(Pyrosequencing)技术检测97例非小细胞肺癌组织和21例支气管扩张或肺大疱等手术切除的良性肺组织中TCF21基因mRNA、蛋白表达及启动子区甲基化状态.结果 97例NSCLC癌组织和21例良性肺组织中分别检测出TCF21基因mRNA阳性表达23例(23.71％)和14例(66.67％);NSCLC癌组织中TCF21蛋白平均表达量灰度值为0.49±1.78,良性肺组织中其平均表达量灰度值为1.48±1.58; NSCLC癌组织和良性肺组织中TCF21基因启动子区均有不同程度的甲基化,且癌组织和良性肺组织中各个位点甲基化频率均有统计学意义,在NSCLC癌组织中第1、5位点平均甲基化频率较高为49.04％和51.37％.结论 TCF21基因mRNA及蛋白表达在NSCLC癌组织和良性肺组织中均有统计学意义,TCF21基因启动子区平均甲基化频率明显高于良性肺组织细胞.
목적 탐토비소세포폐암(NSCLC)중TCF21기인mRNA화단백적표체수평급기계동자구갑기화상태적의의.방법 운용RT-PCR、단백인적법(Western-blot)화초린산측서(Pyrosequencing)기술검측97례비소세포폐암조직화21례지기관확장혹폐대포등수술절제적량성폐조직중TCF21기인mRNA、단백표체급계동자구갑기화상태.결과 97례NSCLC암조직화21례량성폐조직중분별검측출TCF21기인mRNA양성표체23례(23.71％)화14례(66.67％);NSCLC암조직중TCF21단백평균표체량회도치위0.49±1.78,량성폐조직중기평균표체량회도치위1.48±1.58; NSCLC암조직화량성폐조직중TCF21기인계동자구균유불동정도적갑기화,차암조직화량성폐조직중각개위점갑기화빈솔균유통계학의의,재NSCLC암조직중제1、5위점평균갑기화빈솔교고위49.04％화51.37％.결론 TCF21기인mRNA급단백표체재NSCLC암조직화량성폐조직중균유통계학의의,TCF21기인계동자구평균갑기화빈솔명현고우량성폐조직세포.
Objective Background and objective Bioinformatics technology found the TCF21 gene has difference expression in non-small cell lung cancer (NSCLC) and benign lung tissue.To explore TCF21 mRNA and protein expression in nonsmall cell lung cancer and its methylation of the promoter region is the aim of this study.Methods Use RT-PCR,Western blot and Pyrosequencing detected TCF21 gene mRNA,protein and the methylation of the promoter region respectively in 97 cases of non-small cell lung cancer and 21 cases of benign lung tissue.Results TCF21 gene mRNA-positive expression were detected 23 cases (23.71％) and 14 cases (66.67％) in 97 cases of NSCLC cancer tissue and 21 cases of benign lung tissue,the average gray value of TCF21 protein expression levels in NSCLC cancer tissue is 0.49 ± 1.78,while it is 1.48 ± 1.58 in benign lung tissue,the TCF21 gene promoter region have varying degrees methylation in NSCLC cancer tissue and benign lung tissue,and the significant of methylation frequency was found statistically significant between NSCLC cancer tissue and benign lung tissue,also it has higher frequency of 49.04％ and 51.37％ respectively at point 1 and 5 in NSCLC cancer organizations.Conclusion TCF21 gene mRNA and protein expression were statistically significant in NSCLC cancer tissue and benign lung tissue,the TCF21 gene promoter region average methylation frequency was significantly higher than that in benign lung tissue cells.