中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2013年
2期
91-95
,共5页
李博%陈庆良%陈晓波%赵鸿铭%刘建实
李博%陳慶良%陳曉波%趙鴻銘%劉建實
리박%진경량%진효파%조홍명%류건실
干细胞%心肌梗死%干细胞移植%XIAP抗凋亡蛋白%基因修饰
榦細胞%心肌梗死%榦細胞移植%XIAP抗凋亡蛋白%基因脩飾
간세포%심기경사%간세포이식%XIAP항조망단백%기인수식
Adipose derived stem cell%Myocardial infarction%Stem cells transplantation%X-linked inhibitor of apoptosis protein%Gene modification
目的 探讨XIAP修饰的脂肪间充质干细胞(ADMSCs)是否具有更强的活力,能否更好地恢复心功能,及基因修饰干细胞治疗模式的可行性.方法 10周的雄性SD大鼠,体质量200 ～ 300 g,取其腹股沟处脂肪组织,提取脂肪间充质干细胞行体外培养扩增.应用电转染的方法将XIAP表达质粒导入脂肪干细胞内,进行基因修饰.另取61只SD大鼠,年龄和体质量同前,顺序编号,按照随机数字表法完全随机分为对照组(18只)、ADMSCs组(18只)、XIAP修饰ADMSCs组(18只)和未处理组(7只).结扎大鼠冠状动脉左前降支,选取梗死中央及边缘区共5个注射点,按分组每点注射生理盐水、ADMSCs悬液或经XIAP修饰的ADMSCs悬液或不做任何处理.结果 注射4周后,同对照组相比,ADMSCs组的左心室射血分数明显改善[(0.57 ±0.05)对(0.53±0.03),P＜0.05],心肌梗死面积百分比显著减小[(5.17±2.03)对(10.62±3.50),P＜0.05]；与ADMSCs组相比,XIAP修饰的ADMSCs对心肌梗死的治疗作用更为明显,射血分数0.64±0.03,心肌梗死面积百分比3.26±0.95,P＜0.05.结论 ADMSCs能有效恢复梗死后心功能；XIAP修饰的ADMSCs在移植区的生存效率更高,能更好的恢复心功能.
目的 探討XIAP脩飾的脂肪間充質榦細胞(ADMSCs)是否具有更彊的活力,能否更好地恢複心功能,及基因脩飾榦細胞治療模式的可行性.方法 10週的雄性SD大鼠,體質量200 ～ 300 g,取其腹股溝處脂肪組織,提取脂肪間充質榦細胞行體外培養擴增.應用電轉染的方法將XIAP錶達質粒導入脂肪榦細胞內,進行基因脩飾.另取61隻SD大鼠,年齡和體質量同前,順序編號,按照隨機數字錶法完全隨機分為對照組(18隻)、ADMSCs組(18隻)、XIAP脩飾ADMSCs組(18隻)和未處理組(7隻).結扎大鼠冠狀動脈左前降支,選取梗死中央及邊緣區共5箇註射點,按分組每點註射生理鹽水、ADMSCs懸液或經XIAP脩飾的ADMSCs懸液或不做任何處理.結果 註射4週後,同對照組相比,ADMSCs組的左心室射血分數明顯改善[(0.57 ±0.05)對(0.53±0.03),P＜0.05],心肌梗死麵積百分比顯著減小[(5.17±2.03)對(10.62±3.50),P＜0.05]；與ADMSCs組相比,XIAP脩飾的ADMSCs對心肌梗死的治療作用更為明顯,射血分數0.64±0.03,心肌梗死麵積百分比3.26±0.95,P＜0.05.結論 ADMSCs能有效恢複梗死後心功能；XIAP脩飾的ADMSCs在移植區的生存效率更高,能更好的恢複心功能.
목적 탐토XIAP수식적지방간충질간세포(ADMSCs)시부구유경강적활력,능부경호지회복심공능,급기인수식간세포치료모식적가행성.방법 10주적웅성SD대서,체질량200 ～ 300 g,취기복고구처지방조직,제취지방간충질간세포행체외배양확증.응용전전염적방법장XIAP표체질립도입지방간세포내,진행기인수식.령취61지SD대서,년령화체질량동전,순서편호,안조수궤수자표법완전수궤분위대조조(18지)、ADMSCs조(18지)、XIAP수식ADMSCs조(18지)화미처리조(7지).결찰대서관상동맥좌전강지,선취경사중앙급변연구공5개주사점,안분조매점주사생리염수、ADMSCs현액혹경XIAP수식적ADMSCs현액혹불주임하처리.결과 주사4주후,동대조조상비,ADMSCs조적좌심실사혈분수명현개선[(0.57 ±0.05)대(0.53±0.03),P＜0.05],심기경사면적백분비현저감소[(5.17±2.03)대(10.62±3.50),P＜0.05]；여ADMSCs조상비,XIAP수식적ADMSCs대심기경사적치료작용경위명현,사혈분수0.64±0.03,심기경사면적백분비3.26±0.95,P＜0.05.결론 ADMSCs능유효회복경사후심공능；XIAP수식적ADMSCs재이식구적생존효솔경고,능경호적회복심공능.
Objective We want to study the therapeutic efficiency of autologous ADMSC transplantation in myocardial infarction.And we try to find out a good way to improve the therapeutic efficiency by using the combination of gene therapy and cell therapy.Anti-apoptotic protein XIAP was selected to fight against the ischemic environment of myocardic infaction.Methods ADMSC was isolated from rat inguinal fat tissue.ADMSC was cultivated with DMEM.XIAP experession plasmid was elertco-transduced to ADMSC.The anti-apototic function of XIAP was tested by serum stavation induced apotosis.The method of ligation of the left anterior descending artery was used to prepare the Myocardial infarction model.Then rats were randomly separated into three groups to receive direct epicardial injections of normal saline,or ADMSCs cell suspension or XIAP modified ADMSCs cell suspension at five sites in central zones of myocardial infarction and border zone.Cardiac function and the infarct size were evaluated 4 weeks after ADMSCs transplantation.Results West blotting suggest that,XIAP over-expression block serum starvation induced apotosis.It showed that there are significant statistic difference among XIAP modified ADMSC transplantation group,ADMSC transplantation group and control group 4 weeks after myocardial infarction (P ＜ 0.05).Left ventricular ejection fraction(LVEF) showed a significant improvement in ADMSCs transplantation group compared to control group (P ＜0.05).Left ventricular end systolic diameter(LVDs) and left ventricular end diastolic diameter(LVDd) of ADMSCs group were smaller than control group(P ＜ 0.05).The area of myocardial infarction was significantly reduced in the ADMSCs transplantation group compared to the saline group(P ＜0.05).Compared to ADMSCs transplantation group,effect of the XIAP modified ADMSC in rats with myocardial infarction is more obvious.The reduction of LVEF of XIAP modified ADMSCs group was signific antly lower(9％) than the ADMSCs group(16％) (P ＜ 0.01).Infarction area in XIAP modified ADMSCs group(3.26 ±0.95)％ was smaller than ADMSCs group(5.17 ±2.03)％ (P ＜0.05).Conclusion Autologus ADMSC transplantation is an efficient therapeutic tool in myocardial infarction therapy.Over expression of XIAP can partly inhibit lowserum induced apotosis of ADMSC in vitro,and it can improve left ventricular function better in vivo.Over expression XIAP of ADMSC can improve the therapeutic efficiency compare to ADMSC transplantation.
