CAJ | 학술논문

目的探讨腹主动脉缺血预处理对再灌注不同时问血浆丙二醛(MDA)、超氧化物歧化酶(SOD)及脊髓组织中水通道蛋白4(AQP4) mRNA表达的影响.方法将54只体质量189 ～ 207 g雌雄不拘的SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(I/R组)和缺血预处理组(IPC组)3组.采用肾下腹主动脉阻断法,建立脊髓缺血再灌注模型;Sham组6只,手术开始步骤同其他两组,但不作肾下腹主动脉阻断即缝合伤口;I/R组24只,直视下采用动脉夹夹闭腹主动脉段左肾动脉分支起始处下方5 mm处(即肾动脉后型),仅阻断腹主动脉60 min后开放灌注;而IPC组24只,先阻断腹主动脉5min,开放5 min,再阻断60 min后开放灌注.再灌注后12h、1、2、5、10天分别进行神经功能评分,同时观察大鼠血浆MDA、SOD指标的水平,以及预处理对脊髓AQP4 mRNA表达的影响.结果 54只大鼠术后全部存活,I/R组行为学评分降低(P＜0.01),IPC组的神经功能评分在再灌注5天内均高于I/R组(P＜0.01),随着再灌注时间的延长行为学评分逐渐增加.I/R组血浆SOD水平仅在再灌注1天时较Sham组显著下降,随时间延长SOD水平逐渐升高与Sham组无差异,血浆各时间点MDA水平比Sham组低(P ＜0.05);IPC组SOD、MDA水平与Sham组比较没有差异.I/R组再灌注2天时AQP4mRNA表达显著增高(P＜0.01),之后降低至与Sham组无差异;IPC组在再灌注1天时即出现AQP4 mRNA表达明显升高(P＜0.05),之后表达逐渐降低,第5天时与Sham组无差异.IPC AQP4mRNA峰值明显低于I/R组(P＜0.01).结论大鼠腹主动脉缺血再灌注的预处理IPC可能通过减轻由缺血再灌注造成的全身氧化应激反应和下调AQP4mRNA的表达,进而保护脊髓组织.
목적 탐토복주동맥결혈예처리대재관주불동시문혈장병이철(MDA)、초양화물기화매(SOD)급척수조직중수통도단백4(AQP4) mRNA표체적영향.방법 장54지체질량189 ～ 207 g자웅불구적SD대서수궤분위가수술조(Sham조)、결혈재관주조(I/R조)화결혈예처리조(IPC조)3조.채용신하복주동맥조단법,건립척수결혈재관주모형;Sham조6지,수술개시보취동기타량조,단불작신하복주동맥조단즉봉합상구;I/R조24지,직시하채용동맥협협폐복주동맥단좌신동맥분지기시처하방5 mm처(즉신동맥후형),부조단복주동맥60 min후개방관주;이IPC조24지,선조단복주동맥5min,개방5 min,재조단60 min후개방관주.재관주후12h、1、2、5、10천분별진행신경공능평분,동시관찰대서혈장MDA、SOD지표적수평,이급예처리대척수AQP4 mRNA표체적영향.결과 54지대서술후전부존활,I/R조행위학평분강저(P＜0.01),IPC조적신경공능평분재재관주5천내균고우I/R조(P＜0.01),수착재관주시간적연장행위학평분축점증가.I/R조혈장SOD수평부재재관주1천시교Sham조현저하강,수시간연장SOD수평축점승고여Sham조무차이,혈장각시간점MDA수평비Sham조저(P ＜0.05);IPC조SOD、MDA수평여Sham조비교몰유차이.I/R조재관주2천시AQP4mRNA표체현저증고(P＜0.01),지후강저지여Sham조무차이;IPC조재재관주1천시즉출현AQP4 mRNA표체명현승고(P＜0.05),지후표체축점강저,제5천시여Sham조무차이.IPC AQP4mRNA봉치명현저우I/R조(P＜0.01).결론 대서복주동맥결혈재관주적예처리IPC가능통과감경유결혈재관주조성적전신양화응격반응화하조AQP4mRNA적표체,진이보호척수조직.
Objective To investigate the protective effect of Ischemic preconditioning (IPC) on the expression of MDA,SOD and AQP4 mRNA of spinal cord following abdominal aorta occlusion.Methods Fifty-four SD rats were randomly divided into three experimental groups:group Sham,group I/R and group IPC.In group Sham,only laparotomy was performed without further treatment.In group I/R (ischemia reperfusion injury group),a 60 min abdominal aorta occlusion was induced with bulldog clamping.In group IPC,preconditioning of 5 min ischemia and 5 min unclamp by the clamping abdominal aorta were followed by 60 min of reperfusion.At times of 12 hours,1,2,5,10 days after reperfusion behavior scores were respectively given.The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) in blood plasma were determined with enzyme-labeled instrument.Real-time PCR was used to detect the AQP4 mRNA in different treated groups.Results After operation 54 SD rats were all survival.Behavioral score of group I/R decreased until 5 days after reperfusion and the scores of group IPC were all higher than group I/R,by increasing as time went by.The SOD content of group I/R 24-h after reperfusion was dramatically decreased than group Sham,and the MDA contents were all lower(P ＜ 0.05) than group Sham.In group IPC the contents of SOD and MDA showed no significance with group Sham.At the same time,compared with group Sham,AQP4 mRNA expression of group I/R in spinal cord after reperfusion 2 days showed obviously statistical increasing (P ＜ 0.01),and that of group IPC after reperfusion 1 day showed increasing(P ＜0.05),and then AQP4 mRNA expression was kept decreasing until 5 days after reperfusion.The peak value of AQP4 mRNA in group IPC was lower than group I/R (P ＜0.01).Conclusion Ischemia preconditioning in rats could protect spine from I/R injury by reducing oxidative stress and AQP4 mRNA expression.