中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2009年
10期
658-661
,共4页
王谦%杨晓静%单宁宁%侯明%冀学斌%王春燕%朱效娟%杨蕾%张晓琳%彭军%马道新%石艳
王謙%楊曉靜%單寧寧%侯明%冀學斌%王春燕%硃效娟%楊蕾%張曉琳%彭軍%馬道新%石豔
왕겸%양효정%단저저%후명%기학빈%왕춘연%주효연%양뢰%장효림%팽군%마도신%석염
紫癜%血小板减少性%特发性%转录因子T-bet%转录因子GATA-3%白细胞介素18%受体%白细胞介素18
紫癜%血小闆減少性%特髮性%轉錄因子T-bet%轉錄因子GATA-3%白細胞介素18%受體%白細胞介素18
자전%혈소판감소성%특발성%전록인자T-bet%전록인자GATA-3%백세포개소18%수체%백세포개소18
Purpura,thrombocytopenic,idiopathic%Transcription factor T-bet%Transcription factor GATA-3%Intedeukin 18%Interleukin 18 receptor
目的 探讨白细胞介素18(IL-18)及其受体(IL-18R)在特发性血小板减少性紫癜(ITP)患者Th1类细胞因子优势应答中的作用.方法 应用逆转录聚合酶链反应(RT-PCR)检测15例活动期和18例缓解期ITP患者外周血单个核细胞(PBMNC)中转录因子T-bet和GATA-3 mRNA的表达;应用ELISA法检测血浆中IL-18的变化;应用流式细胞术检测CD3+细胞和淋巴细胞表面IL-18R的表达.13名健康志愿者为正常对照.结果 ITP活动期患者PBMNC中T-bet mRNA表达水平(0.069±0.013)明显高于对照组(0.019±0.010)(P＜0.05),而GATA-3 mRNA的表达水平(0.002±0.001)明显低于对照组(0.005±0.002)(P＜0.05);血浆IL-18和CD3+细胞表面IL-18R表达水平较ITP缓解期患者和对照组显著增高.ITP缓解期患者T-bet与GATA-3 mRNA表达水平与对照组比较差异均无统计学意义(P值均＞0.05),T-bet/GATA-3比例基本恢复正常,血浆中IL-18和CD3+细胞表面IL-18R的表达水平也基本恢复正常.结论 ITP活动期患者表现为Th1优势应答,T-bet/GATA-3比例明显失衡,T-bet/GATA-3比例可作为ITP患者Th1类细胞极化的敏感指标;ITP活动期患者IL-18和IL-18R表达上调,可能为ITP患者的治疗提供一个新的治疗靶点.
目的 探討白細胞介素18(IL-18)及其受體(IL-18R)在特髮性血小闆減少性紫癜(ITP)患者Th1類細胞因子優勢應答中的作用.方法 應用逆轉錄聚閤酶鏈反應(RT-PCR)檢測15例活動期和18例緩解期ITP患者外週血單箇覈細胞(PBMNC)中轉錄因子T-bet和GATA-3 mRNA的錶達;應用ELISA法檢測血漿中IL-18的變化;應用流式細胞術檢測CD3+細胞和淋巴細胞錶麵IL-18R的錶達.13名健康誌願者為正常對照.結果 ITP活動期患者PBMNC中T-bet mRNA錶達水平(0.069±0.013)明顯高于對照組(0.019±0.010)(P＜0.05),而GATA-3 mRNA的錶達水平(0.002±0.001)明顯低于對照組(0.005±0.002)(P＜0.05);血漿IL-18和CD3+細胞錶麵IL-18R錶達水平較ITP緩解期患者和對照組顯著增高.ITP緩解期患者T-bet與GATA-3 mRNA錶達水平與對照組比較差異均無統計學意義(P值均＞0.05),T-bet/GATA-3比例基本恢複正常,血漿中IL-18和CD3+細胞錶麵IL-18R的錶達水平也基本恢複正常.結論 ITP活動期患者錶現為Th1優勢應答,T-bet/GATA-3比例明顯失衡,T-bet/GATA-3比例可作為ITP患者Th1類細胞極化的敏感指標;ITP活動期患者IL-18和IL-18R錶達上調,可能為ITP患者的治療提供一箇新的治療靶點.
목적 탐토백세포개소18(IL-18)급기수체(IL-18R)재특발성혈소판감소성자전(ITP)환자Th1류세포인자우세응답중적작용.방법 응용역전록취합매련반응(RT-PCR)검측15례활동기화18례완해기ITP환자외주혈단개핵세포(PBMNC)중전록인자T-bet화GATA-3 mRNA적표체;응용ELISA법검측혈장중IL-18적변화;응용류식세포술검측CD3+세포화림파세포표면IL-18R적표체.13명건강지원자위정상대조.결과 ITP활동기환자PBMNC중T-bet mRNA표체수평(0.069±0.013)명현고우대조조(0.019±0.010)(P＜0.05),이GATA-3 mRNA적표체수평(0.002±0.001)명현저우대조조(0.005±0.002)(P＜0.05);혈장IL-18화CD3+세포표면IL-18R표체수평교ITP완해기환자화대조조현저증고.ITP완해기환자T-bet여GATA-3 mRNA표체수평여대조조비교차이균무통계학의의(P치균＞0.05),T-bet/GATA-3비례기본회복정상,혈장중IL-18화CD3+세포표면IL-18R적표체수평야기본회복정상.결론 ITP활동기환자표현위Th1우세응답,T-bet/GATA-3비례명현실형,T-bet/GATA-3비례가작위ITP환자Th1류세포겁화적민감지표;ITP활동기환자IL-18화IL-18R표체상조,가능위ITP환자적치료제공일개신적치료파점.
Objective To evaluate the role of interleukin(IL)-18 and IL-18 receptor(IL-18R)in the predominant Thl type cytokine response in patients with immune thrombocytopenia(ITP).Methods Fifteen patients with active phase ITP,eighteen in remission and thirteen healthy controls were enrolled in this study.T-bet and GATA-3 mRNA levels in peripheral blood mononueleated cells(PBMNC)were measured by reverse transcfiptase polymerase chain reaction(RT-PCR);the plasma IL-18 level by enzyme linked immunosorbent assay(ELISA),the expression of IL-18R on CD3+ lymphocytes and total lymphocytes by flow cytometry(FCM).Results The T-bet mRNA levels in patients with active phase ITP was 3.572 fold as much as that in the controls(P＜0.05),while the GATA-3 mRNA levels were 0.378 foId of that in controls(P＜O.05).The levels of plasma IL-18 and IL-18R on CD3+ lymphocytes were significantly increased in active ph8se ITP than in remission phase and controls.There was no differenee in ratio of T-bet/GATA-3 between remitted ITP and controls and so was for T-bet mRNA,GATA-3 mRNA,plasma IL-18 and IL-18R on CD3+lymphocytes.Conclusion ITP as a disease of Thl-dominant response there is an unbalance between T-bet and GATA-3 in its active phase:IL-18 and IL-18R being upregulated.
