中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2013年
2期
104-108
,共5页
秦亚溱%程辉%岑建农%耿素霞%李庆华%李小青%林振兴%马道新%乔纯%王云贵%李金兰%李玲娣%黄晓军
秦亞溱%程輝%岑建農%耿素霞%李慶華%李小青%林振興%馬道新%喬純%王雲貴%李金蘭%李玲娣%黃曉軍
진아진%정휘%잠건농%경소하%리경화%리소청%림진흥%마도신%교순%왕운귀%리금란%리령제%황효군
融合基因,bcr-abl(P210)%聚合酶链反应,实时定量%转换系数
融閤基因,bcr-abl(P210)%聚閤酶鏈反應,實時定量%轉換繫數
융합기인,bcr-abl(P210)%취합매련반응,실시정량%전환계수
Fusion gene,bcr-abl (P210)%PCR real-time quantitative%Conversion factor
目的 探讨国内不同医院bcr-abl(P210)转录本水平定量检测值的可比性.方法 2010年4月至2011年8月,10家医院进行了4次样本派发以比对bcr-abl(P210)转录本水平定量检测值.由北京大学人民医院统一制备4个梯度(10倍系列稀释)的bcr-abl(P210) 转录本水平细胞样本,加入TRIzol中,每个梯度每家医院各派发3份平行样品,每份样品细胞数为8×106,各单位分别按照自身实验方案采用实时定量PCR技术检测bcr-abl转录本水平.应用回归方程计算各单位转换系数(CF).结果 每次比对,各医院检测结果之间均存在差异.除个别医院外,各医院检测结果总体一致并与细胞稀释度一致.各家医院的CF均存在波动,其中3家医院比较稳定,4次比对CF范围分别为2.8～5.2、1.2～2.8和2.2～6.8;两家医院的CF变化较大,范围分别为0.76～7.0和2.1～18.7;三家医院各有1～2次检测结果明显偏离实际水平而无法计算出CF,能够算出的CF范围分别为1.9～19.2、3.6～7.6和0.18～14.7;一家医院因为中途更换主要试剂只有2次比对的CF(3.3和5.0).结论 通过比对获得CF能够实现不同医院bcr-abl转录本水平检测值的可比性.检测体系稳定可靠是获得准确CF的前提.
目的 探討國內不同醫院bcr-abl(P210)轉錄本水平定量檢測值的可比性.方法 2010年4月至2011年8月,10傢醫院進行瞭4次樣本派髮以比對bcr-abl(P210)轉錄本水平定量檢測值.由北京大學人民醫院統一製備4箇梯度(10倍繫列稀釋)的bcr-abl(P210) 轉錄本水平細胞樣本,加入TRIzol中,每箇梯度每傢醫院各派髮3份平行樣品,每份樣品細胞數為8×106,各單位分彆按照自身實驗方案採用實時定量PCR技術檢測bcr-abl轉錄本水平.應用迴歸方程計算各單位轉換繫數(CF).結果 每次比對,各醫院檢測結果之間均存在差異.除箇彆醫院外,各醫院檢測結果總體一緻併與細胞稀釋度一緻.各傢醫院的CF均存在波動,其中3傢醫院比較穩定,4次比對CF範圍分彆為2.8～5.2、1.2～2.8和2.2～6.8;兩傢醫院的CF變化較大,範圍分彆為0.76～7.0和2.1～18.7;三傢醫院各有1～2次檢測結果明顯偏離實際水平而無法計算齣CF,能夠算齣的CF範圍分彆為1.9～19.2、3.6～7.6和0.18～14.7;一傢醫院因為中途更換主要試劑隻有2次比對的CF(3.3和5.0).結論 通過比對穫得CF能夠實現不同醫院bcr-abl轉錄本水平檢測值的可比性.檢測體繫穩定可靠是穫得準確CF的前提.
목적 탐토국내불동의원bcr-abl(P210)전록본수평정량검측치적가비성.방법 2010년4월지2011년8월,10가의원진행료4차양본파발이비대bcr-abl(P210)전록본수평정량검측치.유북경대학인민의원통일제비4개제도(10배계렬희석)적bcr-abl(P210) 전록본수평세포양본,가입TRIzol중,매개제도매가의원각파발3빈평행양품,매빈양품세포수위8×106,각단위분별안조자신실험방안채용실시정량PCR기술검측bcr-abl전록본수평.응용회귀방정계산각단위전환계수(CF).결과 매차비대,각의원검측결과지간균존재차이.제개별의원외,각의원검측결과총체일치병여세포희석도일치.각가의원적CF균존재파동,기중3가의원비교은정,4차비대CF범위분별위2.8～5.2、1.2～2.8화2.2～6.8;량가의원적CF변화교대,범위분별위0.76～7.0화2.1～18.7;삼가의원각유1～2차검측결과명현편리실제수평이무법계산출CF,능구산출적CF범위분별위1.9～19.2、3.6～7.6화0.18～14.7;일가의원인위중도경환주요시제지유2차비대적CF(3.3화5.0).결론 통과비대획득CF능구실현불동의원bcr-abl전록본수평검측치적가비성.검측체계은정가고시획득준학CF적전제.
Objective To investigate the comparability of bcr-abl (P210) transcript levels detected in different hospitals. Methods Ten hospitals in China took part in the four times of sample exchange and comparisons from April, 2010 to August, 2011. The exchange samples were prepared by Peking University People's Hospital. Firstly, the BCR-ABL (P210) (+) cells from a newly diagnosed chronic myeloid leukemia patient were 10-fold serially diluted by BCR-ABL (P210) (-) cells and they covered 4 magnitudes. Then, TRIzol reagents were thoroughly mixed with cells in each tube. Every 12 samples (three samples per magnitude) were sent to the other 9 hospitals. The cell number of each sample was 8×106. The detection of bcr-abl transcript levels by real-time quantitative PCR were performed in every hospital according to their own protocols. Conversion factors (CF) were calculated using regression equation. Results Differences in bcr-abl transcript levels did exist among results of 10 hospitals in each comparison. In general, the results of the most of hospitals were in line with the dilutions of cells. CF of every hospital fluctuated. Three hospitals had relatively stable CF, and their ranges were 2.8-5.2, 1.2-2.8 and 2.2-6.8, respectively; two hospitals had unstable CF with ranges 0.76-7.0 and 2.1-18.7; three hospitals couldn't be calculated CF one or two times because of the significant deviation of the results from the actually bcr-abl transcript levels, and their ranges of CF which could be calculated were 1.9-19.2、3.6-7.6 and 0.18-14.7; One hospital only had two CF (3.3 and 5.0) because of the replacement of an important reagent during the period of comparisons. Conclusions Comparability of bcr-abl (P210) transcript levels between different hospitals could be achieved through CF which acquired by sample exchange and comparison. The stable and reliable detection system is the premise to acquire correct CF.
