CAJ | 학술논문

目的探讨多发性骨髓瘤(MM)患者微小RNA-92a(miR-92a)的表达水平及其与染色体del(13q14)和患者预后的关系,并进一步探索miR-92a作用的信号通路.方法收集53例初诊MM患者骨髓标本,用CD138磁珠分选浆细胞,然后用间期荧光原位杂交技术检测染色体del(13q14)情况；用实时定量PCR检测浆细胞中miR-92a的表达；用miR-92a前体转染MM细胞株(LP-1、U266和JJN3),Western blot法检测c-jun蛋白表达水平.结果 53例MM患者中31例(58.5％)染色体del(13q14)阳性；伴有和不伴有del(13q14)的患者miR-92a相对中位表达水平分别为27.364± 2.61和21.87±15.98,两者差异无统计学意义(P＞0.05).中位随访13.5(0.5 ～72.5)个月,miR-92a高表达的患者中位无进展生存期明显短于低表达者(4.5个月对14.0个月,P=0.006).转染miR-92a前体后,MM细胞株(LP-1、U266和JJN3)中c-jun蛋白表达水平呈时间依赖性下降.结论 miR-92a高表达的MM患者预后差,miR-92a表达水平与del(13q14)无相关性,miR-92a有可能通过c-jun途径影响MM的疾病过程.
목적 탐토다발성골수류(MM)환자미소RNA-92a(miR-92a)적표체수평급기여염색체del(13q14)화환자예후적관계,병진일보탐색miR-92a작용적신호통로.방법 수집53례초진MM환자골수표본,용CD138자주분선장세포,연후용간기형광원위잡교기술검측염색체del(13q14)정황；용실시정량PCR검측장세포중miR-92a적표체；용miR-92a전체전염MM세포주(LP-1、U266화JJN3),Western blot법검측c-jun단백표체수평.결과 53례MM환자중31례(58.5％)염색체del(13q14)양성；반유화불반유del(13q14)적환자miR-92a상대중위표체수평분별위27.364± 2.61화21.87±15.98,량자차이무통계학의의(P＞0.05).중위수방13.5(0.5 ～72.5)개월,miR-92a고표체적환자중위무진전생존기명현단우저표체자(4.5개월대14.0개월,P=0.006).전염miR-92a전체후,MM세포주(LP-1、U266화JJN3)중c-jun단백표체수평정시간의뢰성하강.결론 miR-92a고표체적MM환자예후차,miR-92a표체수평여del(13q14)무상관성,miR-92a유가능통과c-jun도경영향MM적질병과정.
Objective To investigate the relationship between the expression level of microRNA 92a (miR-92a) and del(13q14) and the prognosis of MM patients,and to explore the pathway that miR-92a involved.Methods Bone marrow samples from 53 newly diagnosed MM patients were collected,del(13q14)was analyzed by interphase fluorescence in situ hybridization in sorted CD138 positive plasma cell.The expression of miR-92a in plasma cells was measured by quantitative real-time PCR.The expression of c-jun was detected by Western blot in miR-92a transfected MM cell lines (LP-1,U266 and JJN3).Results Of the 53MM patients,del(13q14) was detected in 31 (58.4％) patients.The median levels of miR-92a in MM patients with or without del (13q1 4) were 27.36 ± 2.61 and 21.87 ± 15.98,respectively (P ＞ 0.05).With the median follow-up of 13.5 (0.5-72.5) months,the median duration of progression-free survival of patients with high expression level of miR-92a was significantly shorter than those with low expression level of miR-92a (4.5 months vs 14.0 months,P =0.006).Overexpression of miR-92a in MM cell lines induces time-dependent down-regulation of c-jun.Conclusions High expression of miR-92a was associated with poor prognosis in MM patients.The expression level of miR-92a was not associated with del(13q14),and the effect of miR-92a on the progress of MM might be involved in c-jun pathway.