CAJ | 학술논문

目的探讨内皮祖细胞(EPC)输注对异基因造血干细胞移植(allo-HSCT)小鼠造血重建的影响.方法白消安(BU)/环磷酰胺(CY)预处理后建立C57BL/6供BABL/c小鼠allo-HSCT模型.受鼠随机分为4组:未处理组、BU/CY预处理组、单纯移植组和EPC联合移植组.移植后动态观察受鼠骨髓病理变化,MECA32抗体免疫组化染色观察骨髓窦内皮和微血管的变化；流式细胞术分析骨髓内干、祖细胞比例及血清细胞因子；并行外周血血细胞计数.结果 ①联合EPC输注组小鼠骨髓中造血组织、骨髓窦内皮及微血管的损伤较单纯移植组轻.②予EPC输注后,受鼠骨髓中造血干细胞比例在移植后21天(+21 d)内明显增高,联合移植组小鼠HSC于+14 d达高峰,明显高于单纯移植组(0.1743对0.0787)(P＜0.05),骨髓造血祖细胞移植后持续增高,+21 d时联合EPC组显著高于单纯移植组(0.4550对0.3905)(P＜0.05).③联合移植组外周血白细胞计数早期始终高于单纯移植组,+14d达到高值,(0.74×109/L对0.47×109/L)(P＜0.05),外周血血小板计数于+14 d升至最高,联合EPC移植组显著高于单纯移植组(1228.9×109/L对977.12×109/L)(P＜0.05).结论 alloHSCT联合EPC输注可促进小鼠移植后的造血重建.
목적 탐토내피조세포(EPC)수주대이기인조혈간세포이식(allo-HSCT)소서조혈중건적영향.방법 백소안(BU)/배린선알(CY)예처리후건립C57BL/6공BABL/c소서allo-HSCT모형.수서수궤분위4조:미처리조、BU/CY예처리조、단순이식조화EPC연합이식조.이식후동태관찰수서골수병리변화,MECA32항체면역조화염색관찰골수두내피화미혈관적변화；류식세포술분석골수내간、조세포비례급혈청세포인자；병행외주혈혈세포계수.결과 ①연합EPC수주조소서골수중조혈조직、골수두내피급미혈관적손상교단순이식조경.②여EPC수주후,수서골수중조혈간세포비례재이식후21천(+21 d)내명현증고,연합이식조소서HSC우+14 d체고봉,명현고우단순이식조(0.1743대0.0787)(P＜0.05),골수조혈조세포이식후지속증고,+21 d시연합EPC조현저고우단순이식조(0.4550대0.3905)(P＜0.05).③연합이식조외주혈백세포계수조기시종고우단순이식조,+14d체도고치,(0.74×109/L대0.47×109/L)(P＜0.05),외주혈혈소판계수우+14 d승지최고,연합EPC이식조현저고우단순이식조(1228.9×109/L대977.12×109/L)(P＜0.05).결론 alloHSCT연합EPC수주가촉진소서이식후적조혈중건.
Objective To examine the effects of endothelial progenitor cell (EPC) on hematopoietic reconsititution in allogeneic hematopoietic stem cell transplantation (allo-HSCT) mouse model.Methods Allo-HSCT mouse model was established with condition of BU/CY,in which C57BL/6 (H-2b) and BABL/c (H-2d) mice were used as donors and recipients respectively.Recipients were randomly divided into 4 groups:untreated group,BU/CY condition group,bone marrow transplantation (BMT) group and transplantation of BM cells combined with EPCs (combined transplantation) group.The pathological changes of BM cells following transplantation were dynamically observed.Changes of BM sinusoidal endothelium and angiogenesis were observed by MECA-32 antibody immunohistochemical staining.The proportion of intramedullary stem and progenitor cells and serum cytokines were analyzed by flow cytometry.The numbers of peripheral blood cells were also counted.Results ①Injuries of BM hematopoietic tissue,sinusoidal endothelium and vascular were less severe in combined transplantation group than of BMT group.②EPC infusion significantly increased BM hematopoietic stem cells 21 days after transplantation.The percentage of BM hematopoietic stem cells in combined transplantation group peaked on day + 14,which was higher than of BMT group (0.1743 vs 0.0787) (P ＜ 0.05).The continuously increased percentage of BM hematopoietic progenitor cells in combined transplantation group was significantly higher than in BMT group on day + 21 (0.4550 vs 0.3905) (P ＜ 0.05).③The number of peripheral white blood cells in combined transplantation group was always higher than of BMT group,which reached the peak on day + 14 (0.74 × 109/L to 0.47 × 109/L)(P ＜ 0.05).The peak number of peripheral blood platelets on day + 14 in combined transplantation group was significantly higher than of group BMT (1228.9 × 109/L to 977.12 × 109/L) (P ＜ 0.05).Conclusion Allo-HSCT combined with EPC infusion accelerated hematopoietic reconstitution compared with BMT alone in allo-HSCT mouse model.