中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2013年
7期
614-617
,共4页
张小梅%单宁宁%扈煜%王欣
張小梅%單寧寧%扈煜%王訢
장소매%단저저%호욱%왕흔
血小板减少%脾切除术%TIM-1%TIM-3%Th1细胞
血小闆減少%脾切除術%TIM-1%TIM-3%Th1細胞
혈소판감소%비절제술%TIM-1%TIM-3%Th1세포
Thrombocytopenia%Splenectomy%TIM-1%TIM-3%Th1 cells
目的 探讨T细胞免疫球蛋白黏蛋白分子(TIM)-1、TIM-3及Th2细胞特异性转录因子GATA结合蛋白3(GATA3)、Th1特异性转录因子T-bet在原发免疫性血小板减少症(ITP)患者脾脏单个核细胞中的表达及其临床意义.方法 以17例ITP和10例外伤性脾破裂患者脾标本为研究对象.常规制备脾单个核细胞,应用SYBRw Green实时荧光定量PCR技术检测TIM-1、TIM-3、GATA3、T-betmRNA的表达.结果 ITP患者脾脏单个核细胞中TIM-3 mRNA表达为正常对照组的(29±16)％,差异有统计学意义(P＜0.05),TIM-I mRNA表达是正常对照的(3.20±2.18)倍,差异无统计学意义(P＞0.05),TIM-1/TIM-3比值明显失衡.T-bet mRNA表达较对照组明显上升,而Th2特异性转录因子GATA3 mRNA水平为对照组的14％,差异有统计学意义(P＜0.05),T-bet/GATA-3比值明显增高.结论 TIM-1及TIM-3表达失衡在ITP的免疫紊乱和发生发展中起着一定的作用.
目的 探討T細胞免疫毬蛋白黏蛋白分子(TIM)-1、TIM-3及Th2細胞特異性轉錄因子GATA結閤蛋白3(GATA3)、Th1特異性轉錄因子T-bet在原髮免疫性血小闆減少癥(ITP)患者脾髒單箇覈細胞中的錶達及其臨床意義.方法 以17例ITP和10例外傷性脾破裂患者脾標本為研究對象.常規製備脾單箇覈細胞,應用SYBRw Green實時熒光定量PCR技術檢測TIM-1、TIM-3、GATA3、T-betmRNA的錶達.結果 ITP患者脾髒單箇覈細胞中TIM-3 mRNA錶達為正常對照組的(29±16)％,差異有統計學意義(P＜0.05),TIM-I mRNA錶達是正常對照的(3.20±2.18)倍,差異無統計學意義(P＞0.05),TIM-1/TIM-3比值明顯失衡.T-bet mRNA錶達較對照組明顯上升,而Th2特異性轉錄因子GATA3 mRNA水平為對照組的14％,差異有統計學意義(P＜0.05),T-bet/GATA-3比值明顯增高.結論 TIM-1及TIM-3錶達失衡在ITP的免疫紊亂和髮生髮展中起著一定的作用.
목적 탐토T세포면역구단백점단백분자(TIM)-1、TIM-3급Th2세포특이성전록인자GATA결합단백3(GATA3)、Th1특이성전록인자T-bet재원발면역성혈소판감소증(ITP)환자비장단개핵세포중적표체급기림상의의.방법 이17례ITP화10예외상성비파렬환자비표본위연구대상.상규제비비단개핵세포,응용SYBRw Green실시형광정량PCR기술검측TIM-1、TIM-3、GATA3、T-betmRNA적표체.결과 ITP환자비장단개핵세포중TIM-3 mRNA표체위정상대조조적(29±16)％,차이유통계학의의(P＜0.05),TIM-I mRNA표체시정상대조적(3.20±2.18)배,차이무통계학의의(P＞0.05),TIM-1/TIM-3비치명현실형.T-bet mRNA표체교대조조명현상승,이Th2특이성전록인자GATA3 mRNA수평위대조조적14％,차이유통계학의의(P＜0.05),T-bet/GATA-3비치명현증고.결론 TIM-1급TIM-3표체실형재ITP적면역문란화발생발전중기착일정적작용.
Objective To explore the expression and clinical significance of T cell immunoglobulin mucin (TIM)-1,TIM-3 and T cell-specific transcription factors T-bet and GATA-3 in spleen mononuclear cells in patients with primary immune thrombocytopenia (ITP).Methods The spleen samples were obtained from 17 active ITP patients and 10 controls with spleen traumatic rupture.By using real-time quantitative polymerase chain reaction,the mRNA expressions of TIM-3,TIM1,T-bet and GATA-3 were studied in all subjects.Results TIM-3 mRNA levels of active ITP patients were significantly decreased to (29 ± 16)％ of that of control,TIM-1 mRNA levels of active ITP patients increased to (3.20±2.18) folds of that of control,but the difference was not significant.The ratio of TIM-1/TIM-3 was elevated in active ITP patients.T-bet mRNA levels were up-regulated in ITP patients by (2.82±1.57) folds (P＜0.05) and the expression of GATA3 was decreased by 14％ folds (P＜0.05) compared to controls.The ratio of T-bet/GATA3 were significantly elevated in ITP patients.Conclusion The imbalance between TIM-3 and TIM-1 expression might play an important role in pathogenesis of ITP.
