中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2013年
7期
626-628
,共3页
夏天%李建平%李文倩%王莉%郝星%孙方方%戴昕%阿祥仁%彭海
夏天%李建平%李文倩%王莉%郝星%孫方方%戴昕%阿祥仁%彭海
하천%리건평%리문천%왕리%학성%손방방%대흔%아상인%팽해
白细胞介素27%白细胞介素6%多发性骨髓瘤
白細胞介素27%白細胞介素6%多髮性骨髓瘤
백세포개소27%백세포개소6%다발성골수류
Interleukin 27%Interleukin 6%Multiple myeloma
目的 研究IL-27在多发性骨髓瘤(MM)患者及骨髓瘤细胞系U266、RPMI8226培养上清中的水平及其意义.方法 采用ELISA法检测MM患者及正常对照者血浆中IL-27和IL-6表达水平；采用ELISA法检测U266、RPMI8226细胞培养上清中IL-27表达水平；实时定量RT-PCR方法检测MM患者外周血单个核细胞IL-27 mRNA表达水平.结果 MM患者血浆IL-27水平为(61.82±8.01) ng/L,明显高于正常对照组[(8.29±4.41) ng/L](P＜0.05)；MM患者血浆IL-6水平为(45.62± 1.24) ng/L,也明显高于正常对照组[(2.27±0.18) ng/L] (P＜0.05)；U266、RPMI8226细胞培养上清中IL-27水平分别为(50.06±5.72)和(335.47±41.88) ng/L.RT-PCR结果证实MM患者外周血单个核细胞IL-27 mRNA相对表达水平较正常对照组明显升高,差异有统计学意义(P＜0.05).结论 MM患者高表达IL-27,骨髓瘤细胞系U266、RPMI8226自分泌IL-27,IL-27可能参与MM的发病机制.
目的 研究IL-27在多髮性骨髓瘤(MM)患者及骨髓瘤細胞繫U266、RPMI8226培養上清中的水平及其意義.方法 採用ELISA法檢測MM患者及正常對照者血漿中IL-27和IL-6錶達水平；採用ELISA法檢測U266、RPMI8226細胞培養上清中IL-27錶達水平；實時定量RT-PCR方法檢測MM患者外週血單箇覈細胞IL-27 mRNA錶達水平.結果 MM患者血漿IL-27水平為(61.82±8.01) ng/L,明顯高于正常對照組[(8.29±4.41) ng/L](P＜0.05)；MM患者血漿IL-6水平為(45.62± 1.24) ng/L,也明顯高于正常對照組[(2.27±0.18) ng/L] (P＜0.05)；U266、RPMI8226細胞培養上清中IL-27水平分彆為(50.06±5.72)和(335.47±41.88) ng/L.RT-PCR結果證實MM患者外週血單箇覈細胞IL-27 mRNA相對錶達水平較正常對照組明顯升高,差異有統計學意義(P＜0.05).結論 MM患者高錶達IL-27,骨髓瘤細胞繫U266、RPMI8226自分泌IL-27,IL-27可能參與MM的髮病機製.
목적 연구IL-27재다발성골수류(MM)환자급골수류세포계U266、RPMI8226배양상청중적수평급기의의.방법 채용ELISA법검측MM환자급정상대조자혈장중IL-27화IL-6표체수평；채용ELISA법검측U266、RPMI8226세포배양상청중IL-27표체수평；실시정량RT-PCR방법검측MM환자외주혈단개핵세포IL-27 mRNA표체수평.결과 MM환자혈장IL-27수평위(61.82±8.01) ng/L,명현고우정상대조조[(8.29±4.41) ng/L](P＜0.05)；MM환자혈장IL-6수평위(45.62± 1.24) ng/L,야명현고우정상대조조[(2.27±0.18) ng/L] (P＜0.05)；U266、RPMI8226세포배양상청중IL-27수평분별위(50.06±5.72)화(335.47±41.88) ng/L.RT-PCR결과증실MM환자외주혈단개핵세포IL-27 mRNA상대표체수평교정상대조조명현승고,차이유통계학의의(P＜0.05).결론 MM환자고표체IL-27,골수류세포계U266、RPMI8226자분비IL-27,IL-27가능삼여MM적발병궤제.
Objective To study the expression and significance of IL-27 in patients with multiple myeloma (MM) and in the supernatant of MM cell lines U266 and RPMI8226 cells culture medium.Methods A total of 20 MM patients and 20 controls were enrolled in this study.The expressions of IL-27 and IL-6 in MM patient' s blood plasma,and the expression of IL-27 in U266 and RPMI8226 culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA).The mRNA expression of IL-27 in mononuclear cells of MM patients' peripheral blood was measured by real-time quantitative polymerase chain reaction (RT-PCR).Results The levels ofIL-27 in plasma of MM patients and normal controls were (61.82±8.01) ng/L and (8.29±4.41) ng/L (P＜0.05),and those of IL-6 were (45.62± 1.24) ng/L and (2.27 ± 0.18) ng/L (P＜0.05),respectively.The levels of IL-27 in U266 and RPMI8226 culture supematant were (50.06±5.72) ng/L and (335.47±41.88) ng/L.RT-PCR revealed that the levels of IL-27 mRNA were up-regulated in MM patients compared to controls.Conclusion High expression of IL-27 is observed in MM patients and MM cell lines U266 and RPMI8226 cells.IL-27 may play a important role in pathogenesis of MM.
