中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2013年
7期
629-631
,共3页
沈军%陈因%王国镇%马亮%韩呈武
瀋軍%陳因%王國鎮%馬亮%韓呈武
침군%진인%왕국진%마량%한정무
脑脊液%细胞计数及分类%血细胞分析仪%手工计数
腦脊液%細胞計數及分類%血細胞分析儀%手工計數
뇌척액%세포계수급분류%혈세포분석의%수공계수
Cerebrospinal fluid%Cell count and classification%Blood cell analyzer%Manual counting
目的 评价XT-4000i自动血细胞分析仪在脑脊液(CSF)白细胞计数及分类应用中的价值.方法 采用XT-4000i血细胞分析仪体液分析模式对200份脑脊液标本进行计数和分类分析,并与人工显微镜计数法进行结果比对.白细胞分类手工法采用瑞氏染色,仪器法采用荧光染色和流式细胞术原理进行分类.结果 两种方法对脑脊液标本白细胞计数、红细胞计数、单个核及多个核细胞的绝对计数检测差异无统计学意义(P>0.05),并且两种方法具有良好的相关性(白细胞计数r=0.987、红细胞计数r=0.999、单个核细胞绝对计数r=0.981,多个核细胞绝对计数r=0.983).仪器检测中存在高荧光染色的标本瑞氏染色后镜检可见肿瘤细胞,与临床诊断一致.结论 应用XT-4000i自动血细胞分析仪的体液模式进行脑脊液细胞计数、分类快速准确,同时还能为检测肿瘤细胞提供初筛信息.自动血细胞分析仪体液模式检测脑脊液白细胞计数及分类在临床应用中具有推广价值.
目的 評價XT-4000i自動血細胞分析儀在腦脊液(CSF)白細胞計數及分類應用中的價值.方法 採用XT-4000i血細胞分析儀體液分析模式對200份腦脊液標本進行計數和分類分析,併與人工顯微鏡計數法進行結果比對.白細胞分類手工法採用瑞氏染色,儀器法採用熒光染色和流式細胞術原理進行分類.結果 兩種方法對腦脊液標本白細胞計數、紅細胞計數、單箇覈及多箇覈細胞的絕對計數檢測差異無統計學意義(P>0.05),併且兩種方法具有良好的相關性(白細胞計數r=0.987、紅細胞計數r=0.999、單箇覈細胞絕對計數r=0.981,多箇覈細胞絕對計數r=0.983).儀器檢測中存在高熒光染色的標本瑞氏染色後鏡檢可見腫瘤細胞,與臨床診斷一緻.結論 應用XT-4000i自動血細胞分析儀的體液模式進行腦脊液細胞計數、分類快速準確,同時還能為檢測腫瘤細胞提供初篩信息.自動血細胞分析儀體液模式檢測腦脊液白細胞計數及分類在臨床應用中具有推廣價值.
목적 평개XT-4000i자동혈세포분석의재뇌척액(CSF)백세포계수급분류응용중적개치.방법 채용XT-4000i혈세포분석의체액분석모식대200빈뇌척액표본진행계수화분류분석,병여인공현미경계수법진행결과비대.백세포분류수공법채용서씨염색,의기법채용형광염색화류식세포술원리진행분류.결과 량충방법대뇌척액표본백세포계수、홍세포계수、단개핵급다개핵세포적절대계수검측차이무통계학의의(P>0.05),병차량충방법구유량호적상관성(백세포계수r=0.987、홍세포계수r=0.999、단개핵세포절대계수r=0.981,다개핵세포절대계수r=0.983).의기검측중존재고형광염색적표본서씨염색후경검가견종류세포,여림상진단일치.결론 응용XT-4000i자동혈세포분석의적체액모식진행뇌척액세포계수、분류쾌속준학,동시환능위검측종류세포제공초사신식.자동혈세포분석의체액모식검측뇌척액백세포계수급분류재림상응용중구유추엄개치.
Objective To evaluate the application of XT-4000i automatic blood cell analyzer in white cell count and classification of cerebrospinal fluid (CSF).Methods The fluid model of XT-4000i automatic blood cell analyzer was directly used to detect the white cell count and classification in 200 samples of CSF,and compared with the results obtained by manually microscopic counting method.White blood cell classification was performed by manual method under microscope with Wright' s staining,and instrumental method with fluorescence staining and flow cytometry.Results There is no statistical difference between instrumental and manual method in detecting the absolute counting of WBC,RBC,mononuclear cell and multinucleate cells (P>0.05),and there is a good correlation between the two methods (r=0.987,0.999,0.981 and 0.983 for WBC,RBC,mononuclear cell and multinucleate cell counts).Tumor cells in the samples with high fluorescent staining by instrumental method can be identified with Wright' s staining by microscope method,which were consistent with the clinical diagnosis.Conclusions The fluid model of XT-4000i automatic blood cell analyzer was rapid and accurate in CSF white cell count and classification,and it also can provide preliminary information for tumor cells screening.The fluid model of XT-4000i automatic blood cell analyzer in white cell count and classification of CSF has the value of popularization in clinical application.