中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2014年
5期
408-413
,共6页
田小波%张博%李茜%翟侃%许建%常江%乔艳%周宇灵%黄理明
田小波%張博%李茜%翟侃%許建%常江%喬豔%週宇靈%黃理明
전소파%장박%리천%적간%허건%상강%교염%주우령%황리명
微RNAs%多态性,单核苷酸%淋巴瘤,T细胞%预后
微RNAs%多態性,單覈苷痠%淋巴瘤,T細胞%預後
미RNAs%다태성,단핵감산%림파류,T세포%예후
microRNAs%Polymorphism,single nucleotide%Lymphoma,T-Cell%Prognosis
目的 探讨microRNA相关基因DROSHA rs10719、DROSHA rs6877842、DICER1rs3742330、GEMIN4 rs3744741单核苷酸多态性(SNP)与T细胞淋巴瘤(TCL)患者预后的关系.方法 收集163例TCL患者外周血标本,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测4个SNP基因型,分析这些SNP与患者完全缓解(CR)率和总体生存(OS)率的相关性.结果 携带DROSHA rs6877842 CG基因型的患者CR率显著高于携带CC基因型的患者(OR=0.07,95％ CI0.01～0.72,P=0.026);携带DICER1 rs3742330 GG基因型患者的CR率显著高于携带GA基因型(OR=0.15,95％CI 0.02～0.97,P=0.047)或AA基因型(OR=0.11,95％ CI0.02～0.71,P=0.020)的患者.携带GG基因型的患者OS率明显优于携带GA基因型(HR=9.02,95％ CI 1.22～66.92,P=0.031)或AA基因型(HR=8.77,95％ CI 1.19～64.67,P=0.033)的患者.DROSHA rs 10719和GEMIN4 rs3744741与CR、OS率均无显著相关性.结论 DROSHA rs6877842和DICER1 rs3742330是TCL患者CR率相关的独立预后因素,DICER1 rs3742330也是TCL患者OS率相关的独立预后因素.
目的 探討microRNA相關基因DROSHA rs10719、DROSHA rs6877842、DICER1rs3742330、GEMIN4 rs3744741單覈苷痠多態性(SNP)與T細胞淋巴瘤(TCL)患者預後的關繫.方法 收集163例TCL患者外週血標本,採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)技術檢測4箇SNP基因型,分析這些SNP與患者完全緩解(CR)率和總體生存(OS)率的相關性.結果 攜帶DROSHA rs6877842 CG基因型的患者CR率顯著高于攜帶CC基因型的患者(OR=0.07,95％ CI0.01～0.72,P=0.026);攜帶DICER1 rs3742330 GG基因型患者的CR率顯著高于攜帶GA基因型(OR=0.15,95％CI 0.02～0.97,P=0.047)或AA基因型(OR=0.11,95％ CI0.02～0.71,P=0.020)的患者.攜帶GG基因型的患者OS率明顯優于攜帶GA基因型(HR=9.02,95％ CI 1.22～66.92,P=0.031)或AA基因型(HR=8.77,95％ CI 1.19～64.67,P=0.033)的患者.DROSHA rs 10719和GEMIN4 rs3744741與CR、OS率均無顯著相關性.結論 DROSHA rs6877842和DICER1 rs3742330是TCL患者CR率相關的獨立預後因素,DICER1 rs3742330也是TCL患者OS率相關的獨立預後因素.
목적 탐토microRNA상관기인DROSHA rs10719、DROSHA rs6877842、DICER1rs3742330、GEMIN4 rs3744741단핵감산다태성(SNP)여T세포림파류(TCL)환자예후적관계.방법 수집163례TCL환자외주혈표본,채용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술검측4개SNP기인형,분석저사SNP여환자완전완해(CR)솔화총체생존(OS)솔적상관성.결과 휴대DROSHA rs6877842 CG기인형적환자CR솔현저고우휴대CC기인형적환자(OR=0.07,95％ CI0.01～0.72,P=0.026);휴대DICER1 rs3742330 GG기인형환자적CR솔현저고우휴대GA기인형(OR=0.15,95％CI 0.02～0.97,P=0.047)혹AA기인형(OR=0.11,95％ CI0.02～0.71,P=0.020)적환자.휴대GG기인형적환자OS솔명현우우휴대GA기인형(HR=9.02,95％ CI 1.22～66.92,P=0.031)혹AA기인형(HR=8.77,95％ CI 1.19～64.67,P=0.033)적환자.DROSHA rs 10719화GEMIN4 rs3744741여CR、OS솔균무현저상관성.결론 DROSHA rs6877842화DICER1 rs3742330시TCL환자CR솔상관적독립예후인소,DICER1 rs3742330야시TCL환자OS솔상관적독립예후인소.
Objective To analyze the association of micoRNA-related genes DROSHA single nucleotide polymorphisms (SNP) rs10719 and rs6877842,DICER1 rs3742330 and GEMIN4 rs3744741 with prognosis of T-cell lymphoma.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genotypes of the above 4 SNPs and their associations with complete remission (CR) rate and overall survival (OS) in 163 patients with TCL.Results Patients carrying the rs6877842 CG genotype had a significantly higher CR rate compared with those carrying the CC genotype (OR=0.07,95％ CI 0.01-0.72,P=0.026); the same for patients carrying the DICER1 rs3742330 GG genotype compared with those carrying the GA genotype (OR=0.15,95％ CI 0.02-0.97,P=0.047) or the AA genotype (OR=0.11,95％ CI 0.02-0.71,P=0.020).In addition,patients with the DICER1 rs3742330 GG genotype had a significantly improved OS compared with those carrying the GA (HR=9.02,95％ CI 1.22-66.92,P=0.031) or AA genotype (HR=8.77,95％ CI 1.19-64.67,P=0.033).The other two SNPs of rs10719 and rs3744741 had no significant association with CR or OS.Conclusion DROSHA rs6877842 and DICER1 rs3742330 were independent factors for TCL CR,and DICER1 rs3742330 was also an independent prognostic factor for TCL OS.
