中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2014年
10期
880-884
,共5页
李莉娟%张连生%柴晔%曾鹏云%吴重阳%岳玲玲%白俊%郝正栋%胡婉丽
李莉娟%張連生%柴曄%曾鵬雲%吳重暘%嶽玲玲%白俊%郝正棟%鬍婉麗
리리연%장련생%시엽%증붕운%오중양%악령령%백준%학정동%호완려
白血病%肿瘤消退,自行性%树突细胞%感染
白血病%腫瘤消退,自行性%樹突細胞%感染
백혈병%종류소퇴,자행성%수돌세포%감염
Leukemia%Neoplasm regression,spontaneous%Dendritic cells%Infection
目的 探讨浆样树突细胞(pDC)在感染诱发白血病自发缓解(SR)中的免疫作用.方法 分离白血病SR患者及健康人外周血单个核细胞,应用免疫磁珠阴性选择及流式细胞阳性选择双次分选法,获得纯化的人pDC和髓样树突细胞(mDC).将pDC分别与不同细菌共培养,观察其活化情况,MTT法检测T细胞增殖能力,ELISA法检测细胞因子(IFN-α、IL-12、IFN-γ、IL-2、IL-4、IL-10)水平的变化.结果 金黄色葡萄球菌、铜绿假单胞菌能刺激白血病SR患者pDC分泌大量IFN-α,孵育48 h,pDC上清液中IFN-α水平分别为(15.34±2.91)ng/ml和(10.38±1.41)ng/ml,较阴性对照组(脂多糖+pDC)[(1.36±0.13)ng/ml]明显升高(P值均＜0.01).活化的pDC能促使初始CD4+T细胞向Th1细胞分化[培养上清IFN-γ水平分别为(2.16±0.37)ng/ml和(2.73±1.11)ng/ml,平行对照组(脂多糖活化mDC+T细胞)为(2.55±0.23)ng/ml,P＞0.05],并使T细胞增殖能力增强,细胞增殖指数分别为4.36及4.05,高于非活化组的1.23及0.13(P＜0.01).金黄色葡萄球菌、铜绿假单胞菌对健康人来源pDC具有同样的活化能力.结论 金黄色葡萄球菌、铜绿假单胞菌活化的pDC在感染诱导的白血病SR中可能发挥重要作用.
目的 探討漿樣樹突細胞(pDC)在感染誘髮白血病自髮緩解(SR)中的免疫作用.方法 分離白血病SR患者及健康人外週血單箇覈細胞,應用免疫磁珠陰性選擇及流式細胞暘性選擇雙次分選法,穫得純化的人pDC和髓樣樹突細胞(mDC).將pDC分彆與不同細菌共培養,觀察其活化情況,MTT法檢測T細胞增殖能力,ELISA法檢測細胞因子(IFN-α、IL-12、IFN-γ、IL-2、IL-4、IL-10)水平的變化.結果 金黃色葡萄毬菌、銅綠假單胞菌能刺激白血病SR患者pDC分泌大量IFN-α,孵育48 h,pDC上清液中IFN-α水平分彆為(15.34±2.91)ng/ml和(10.38±1.41)ng/ml,較陰性對照組(脂多糖+pDC)[(1.36±0.13)ng/ml]明顯升高(P值均＜0.01).活化的pDC能促使初始CD4+T細胞嚮Th1細胞分化[培養上清IFN-γ水平分彆為(2.16±0.37)ng/ml和(2.73±1.11)ng/ml,平行對照組(脂多糖活化mDC+T細胞)為(2.55±0.23)ng/ml,P＞0.05],併使T細胞增殖能力增彊,細胞增殖指數分彆為4.36及4.05,高于非活化組的1.23及0.13(P＜0.01).金黃色葡萄毬菌、銅綠假單胞菌對健康人來源pDC具有同樣的活化能力.結論 金黃色葡萄毬菌、銅綠假單胞菌活化的pDC在感染誘導的白血病SR中可能髮揮重要作用.
목적 탐토장양수돌세포(pDC)재감염유발백혈병자발완해(SR)중적면역작용.방법 분리백혈병SR환자급건강인외주혈단개핵세포,응용면역자주음성선택급류식세포양성선택쌍차분선법,획득순화적인pDC화수양수돌세포(mDC).장pDC분별여불동세균공배양,관찰기활화정황,MTT법검측T세포증식능력,ELISA법검측세포인자(IFN-α、IL-12、IFN-γ、IL-2、IL-4、IL-10)수평적변화.결과 금황색포도구균、동록가단포균능자격백혈병SR환자pDC분비대량IFN-α,부육48 h,pDC상청액중IFN-α수평분별위(15.34±2.91)ng/ml화(10.38±1.41)ng/ml,교음성대조조(지다당+pDC)[(1.36±0.13)ng/ml]명현승고(P치균＜0.01).활화적pDC능촉사초시CD4+T세포향Th1세포분화[배양상청IFN-γ수평분별위(2.16±0.37)ng/ml화(2.73±1.11)ng/ml,평행대조조(지다당활화mDC+T세포)위(2.55±0.23)ng/ml,P＞0.05],병사T세포증식능력증강,세포증식지수분별위4.36급4.05,고우비활화조적1.23급0.13(P＜0.01).금황색포도구균、동록가단포균대건강인래원pDC구유동양적활화능력.결론 금황색포도구균、동록가단포균활화적pDC재감염유도적백혈병SR중가능발휘중요작용.
Objective To explore the immuno-effect of plasmacytoid dendritic cells (pDC) on bacteria infection induced spontaneous remission (SR) of leukemia.Methods Both pDC and myeloid dendritic cells (mDC) were isolated and purified from leukemic patient with SR and healthy donor by combination of immunomagnetic beads and flow cytometry.pDC were cultured in RPMI1640 medium and stimulated with different bacteria.The T cells proliferation was detected by MTT,and cytokine production by ELISA kits.Results The human bacterial pathogen Staphylococcus aureus and Pseudomonas aeruginosa stimulation for 48 h resulted in the maturation of pDC with production of high quantity of IFN-α at (15.34±2.91) ng/ml and (10.38± 1.41) ng/ml,respectively,comparing with that of negative group at (1.36±0.13) ng/ml (P ＜ 0.01).Activated pDC could promote the differentiation of na?ve CD4+ T cells to Thl cells with secretion of IFN-γ at (2.16±0.37) ng/ml and (2.73± 1.11) ng/ml,respectively,comparing with that of positive control at (2.55 ± 0.23) ng/ml (P＞ 0.05).Activated pDC showed higher T cell stimulatory capacities [proliferation index (PI) was 4.36 and 4.05,respectively] than that of non-activated pDC (PI was 1.23 and 0.13,respectively) (P＜0.01).Conclusion Staphylococcus aureus and Pseudomonas aeruginosa activated pDC may play a key role in SR of leukemia following severe infections.
