中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2012年
6期
610-614
,共5页
席晓婷%陈前波%唐志萍%李燕
席曉婷%陳前波%唐誌萍%李燕
석효정%진전파%당지평%리연
脉络膜新生血管化/预防和控制%激肽释放酶类/拮抗剂和抑制剂%动物实验
脈絡膜新生血管化/預防和控製%激肽釋放酶類/拮抗劑和抑製劑%動物實驗
맥락막신생혈관화/예방화공제%격태석방매류/길항제화억제제%동물실험
Choroidal neovascularization/prevention & control%Kallikreins/antagonists & inhibitors%Animal experimentation
目的 观察激肽释放酶结合蛋白(KBP)对脉络膜新生血管(CNV)的抑制作用.方法 40只Brown Norway大鼠随机分为KBP组和对照组,每组均为20只,右眼为实验眼.采用532 nm激光建立CNV模型.激光光凝后1周行荧光素眼底血管造影(FFA)检查.检查后第2天,KBP组大鼠玻璃体腔注射浓度为4 mg/ml的KBP溶液5μl,对照组大鼠玻璃体腔注射5μl去离子水.两组大鼠注射后1、2、3周行FFA检查,各时间点检查后,分别摘除5只大鼠眼球制作石蜡切片进行苏木精-伊红染色、血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)免疫组织化学3-氨基-9-乙基咔唑染色.应用Image Pro Plus6.0图像分析系统,测量CNV渗漏面积和激光光斑区累积吸光度[A,旧称光密度(OD)]值.结果 激光光凝后1周,FFA检查结果显示CNV形成,可见荧光渗漏.注射后1、2、3周,KBP组大鼠CNV渗漏逐渐减少；对照组大鼠CNV渗漏随观察时间延长而增加.光学显微镜观察发现,KBP组光凝斑面积较对照组光凝斑面积逐渐缩小,新生血管亦逐渐萎缩；对照组光凝斑处新生血管持续存在.免疫组织化学检查结果显示,两组大鼠激光光凝后1周,视网膜VEGF(F=1.29)、PEDF(F=6.29)表达平均累积A值比较,差异均无统计学意义(P＞0.05).注射后1、2、3周,两组大鼠视网膜VEGF、PEDF表达平均累积A值比较,差异均有统计学意义(VEGF:F=14.16、66.89、24.34,PEDF:F=4.22、62.04、233.05;P＜0.001).结论 玻璃体腔注射KBP能有效抑制CNV的形成和发展.
目的 觀察激肽釋放酶結閤蛋白(KBP)對脈絡膜新生血管(CNV)的抑製作用.方法 40隻Brown Norway大鼠隨機分為KBP組和對照組,每組均為20隻,右眼為實驗眼.採用532 nm激光建立CNV模型.激光光凝後1週行熒光素眼底血管造影(FFA)檢查.檢查後第2天,KBP組大鼠玻璃體腔註射濃度為4 mg/ml的KBP溶液5μl,對照組大鼠玻璃體腔註射5μl去離子水.兩組大鼠註射後1、2、3週行FFA檢查,各時間點檢查後,分彆摘除5隻大鼠眼毬製作石蠟切片進行囌木精-伊紅染色、血管內皮生長因子(VEGF)和色素上皮衍生因子(PEDF)免疫組織化學3-氨基-9-乙基咔唑染色.應用Image Pro Plus6.0圖像分析繫統,測量CNV滲漏麵積和激光光斑區纍積吸光度[A,舊稱光密度(OD)]值.結果 激光光凝後1週,FFA檢查結果顯示CNV形成,可見熒光滲漏.註射後1、2、3週,KBP組大鼠CNV滲漏逐漸減少；對照組大鼠CNV滲漏隨觀察時間延長而增加.光學顯微鏡觀察髮現,KBP組光凝斑麵積較對照組光凝斑麵積逐漸縮小,新生血管亦逐漸萎縮；對照組光凝斑處新生血管持續存在.免疫組織化學檢查結果顯示,兩組大鼠激光光凝後1週,視網膜VEGF(F=1.29)、PEDF(F=6.29)錶達平均纍積A值比較,差異均無統計學意義(P＞0.05).註射後1、2、3週,兩組大鼠視網膜VEGF、PEDF錶達平均纍積A值比較,差異均有統計學意義(VEGF:F=14.16、66.89、24.34,PEDF:F=4.22、62.04、233.05;P＜0.001).結論 玻璃體腔註射KBP能有效抑製CNV的形成和髮展.
목적 관찰격태석방매결합단백(KBP)대맥락막신생혈관(CNV)적억제작용.방법 40지Brown Norway대서수궤분위KBP조화대조조,매조균위20지,우안위실험안.채용532 nm격광건립CNV모형.격광광응후1주행형광소안저혈관조영(FFA)검사.검사후제2천,KBP조대서파리체강주사농도위4 mg/ml적KBP용액5μl,대조조대서파리체강주사5μl거리자수.량조대서주사후1、2、3주행FFA검사,각시간점검사후,분별적제5지대서안구제작석사절편진행소목정-이홍염색、혈관내피생장인자(VEGF)화색소상피연생인자(PEDF)면역조직화학3-안기-9-을기잡서염색.응용Image Pro Plus6.0도상분석계통,측량CNV삼루면적화격광광반구루적흡광도[A,구칭광밀도(OD)]치.결과 격광광응후1주,FFA검사결과현시CNV형성,가견형광삼루.주사후1、2、3주,KBP조대서CNV삼루축점감소；대조조대서CNV삼루수관찰시간연장이증가.광학현미경관찰발현,KBP조광응반면적교대조조광응반면적축점축소,신생혈관역축점위축；대조조광응반처신생혈관지속존재.면역조직화학검사결과현시,량조대서격광광응후1주,시망막VEGF(F=1.29)、PEDF(F=6.29)표체평균루적A치비교,차이균무통계학의의(P＞0.05).주사후1、2、3주,량조대서시망막VEGF、PEDF표체평균루적A치비교,차이균유통계학의의(VEGF:F=14.16、66.89、24.34,PEDF:F=4.22、62.04、233.05;P＜0.001).결론 파리체강주사KBP능유효억제CNV적형성화발전.
Objective To observe the inhibitory effect of kallikrein-binding protein (KBP) on choroidal neovascularization.Methods Forty Brown Norway rats were randomly divided into the KBP groups and the control group,20 rats in each group,the right eye as the experimental eye.The rats were photocoagulated by 532 nm laser to induce CNV model.One week after laser photocoagulation,the rats were received FFA examination.At the second day after FFA examination,the rats of KBP group were received an intravitreal injection of KBP 5 μl (4 mg/ml KBP).The same volume of deionized water was injected into the rats in the control group.The rats of two groups received FFA examination at one,two and three weeks after injection.The expressions of vascular endothelial growth factor and pigment epithelium derived factor were observed using hematoxylin and eosin stain and immunohistochemistry stain.CNV leakage area and the cumulative absorbance of laser spot area were analyzed by Image-Pro plus 6.0 software.Results FFA examination showed that there were CNV and fluorescence leakage at one week after laser photocoagulation; one,two and three weeks after injection,the leakage decreased gradually in KBP group,but increased with time in control group.Compared with control group,the spot area and CNV in KBP group reduced gradually,but CNV was always there in control group.The differences of VEGF (F=1.29)and PEDF (F=6.29) expressions at one week after laser photocoagulation were not statistically significant (P＞0.05).The differences of VEGF and PEDF expressions at one,two and three weeks after injection were statistically significant(VEGF:F=14.16,66.89,24.34; PEDF:F=4.22,62.04,233.05; P＜0.001).Conclusion Intravitreal injection with KBP can inhibit CNV.
