中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2013年
1期
26-29
,共4页
刘涛%巩增锋%尹洁%景玉宏
劉濤%鞏增鋒%尹潔%景玉宏
류도%공증봉%윤길%경옥굉
糖尿病视网膜病变/药物疗法%藜芦属/药物作用%毛细血管通透性/药物作用%动物实验
糖尿病視網膜病變/藥物療法%藜蘆屬/藥物作用%毛細血管通透性/藥物作用%動物實驗
당뇨병시망막병변/약물요법%려호속/약물작용%모세혈관통투성/약물작용%동물실험
Diabetic retinopathy/drug therapy%Veratrum/drug effects%Capillary permeability/ drug effects%Animal experimentation
目的 观察白藜芦醇对糖尿病视网膜血管病变的作用.方法 健康Sprague-Dawley雄性大鼠45只,随机分为白藜芦醇治疗组、治疗对照组及正常对照组,每组15只大鼠.白藜芦醇治疗组、治疗对照组大鼠股静脉注射链脲佐菌素(STZ)溶液建模,正常对照组大鼠股静脉注射等量无菌生理盐水.白藜芦醇治疗组、治疗对照组大鼠给予高脂饲料喂养.白藜芦醇治疗组每天以75 mg/kg的剂量灌胃白藜芦醇2次,治疗对照组每天以等体积无菌水灌胃2次；均正常摄食、摄水.持续治疗4个月后,各组大鼠经股静脉采血2 ml,采集房水50μl.检测空腹血糖、房水葡萄糖、血浆胆固醇及血浆甘油三酯水平.利用伊凡思蓝(EB)测定各组大鼠视网膜血管通透性.分离大鼠视网膜,观察各组大鼠视网膜血管中周细胞数量的变化；提取总蛋白,采用蛋白质免疫印迹法(Western blot)检测各组大鼠视网膜中血管内皮生长因子(VEGF)的表达.结果 持续治疗4个月后,3组大鼠空腹血糖、房水葡萄糖、血浆总胆固醇及血浆甘油三酯水平比较,治疗对照组较正常对照组明显升高；白藜芦醇治疗组与治疗对照组比较,除血浆总胆固醇无明显差异外,其余指标均明显降低；3组间各指标差异均有统计学意义(F=152.809、65.230、3.861、15.059,P＜0.05).3组大鼠视网膜血管通透性比较,治疗对照组较正常对照组高,白藜芦醇治疗组较治疗对照组低；3组间差异有统计学意义(F=11.626,P＜0.05).3组大鼠视网膜血管中周细胞数量比较,治疗对照组较正常对照组明显减少,白藜芦醇治疗组较治疗对照组明显增加；3组间差异有统计学意义(F=43.284,P＜0.05).3组大鼠视网膜VEGF表达水平比较,治疗对照组较正常对照组明显升高,白藜芦醇治疗组较治疗对照组明显降低；3组间差异有统计学意义(F=14.017,P＜0.05).结论 白藜芦醇能通过降低空腹血糖、房水葡萄糖及血浆甘油三酯水平,抑制VEGF高表达而改善糖尿病视网膜血管的结构和功能异常.
目的 觀察白藜蘆醇對糖尿病視網膜血管病變的作用.方法 健康Sprague-Dawley雄性大鼠45隻,隨機分為白藜蘆醇治療組、治療對照組及正常對照組,每組15隻大鼠.白藜蘆醇治療組、治療對照組大鼠股靜脈註射鏈脲佐菌素(STZ)溶液建模,正常對照組大鼠股靜脈註射等量無菌生理鹽水.白藜蘆醇治療組、治療對照組大鼠給予高脂飼料餵養.白藜蘆醇治療組每天以75 mg/kg的劑量灌胃白藜蘆醇2次,治療對照組每天以等體積無菌水灌胃2次；均正常攝食、攝水.持續治療4箇月後,各組大鼠經股靜脈採血2 ml,採集房水50μl.檢測空腹血糖、房水葡萄糖、血漿膽固醇及血漿甘油三酯水平.利用伊凡思藍(EB)測定各組大鼠視網膜血管通透性.分離大鼠視網膜,觀察各組大鼠視網膜血管中週細胞數量的變化；提取總蛋白,採用蛋白質免疫印跡法(Western blot)檢測各組大鼠視網膜中血管內皮生長因子(VEGF)的錶達.結果 持續治療4箇月後,3組大鼠空腹血糖、房水葡萄糖、血漿總膽固醇及血漿甘油三酯水平比較,治療對照組較正常對照組明顯升高；白藜蘆醇治療組與治療對照組比較,除血漿總膽固醇無明顯差異外,其餘指標均明顯降低；3組間各指標差異均有統計學意義(F=152.809、65.230、3.861、15.059,P＜0.05).3組大鼠視網膜血管通透性比較,治療對照組較正常對照組高,白藜蘆醇治療組較治療對照組低；3組間差異有統計學意義(F=11.626,P＜0.05).3組大鼠視網膜血管中週細胞數量比較,治療對照組較正常對照組明顯減少,白藜蘆醇治療組較治療對照組明顯增加；3組間差異有統計學意義(F=43.284,P＜0.05).3組大鼠視網膜VEGF錶達水平比較,治療對照組較正常對照組明顯升高,白藜蘆醇治療組較治療對照組明顯降低；3組間差異有統計學意義(F=14.017,P＜0.05).結論 白藜蘆醇能通過降低空腹血糖、房水葡萄糖及血漿甘油三酯水平,抑製VEGF高錶達而改善糖尿病視網膜血管的結構和功能異常.
목적 관찰백려호순대당뇨병시망막혈관병변적작용.방법 건강Sprague-Dawley웅성대서45지,수궤분위백려호순치료조、치료대조조급정상대조조,매조15지대서.백려호순치료조、치료대조조대서고정맥주사련뇨좌균소(STZ)용액건모,정상대조조대서고정맥주사등량무균생리염수.백려호순치료조、치료대조조대서급여고지사료위양.백려호순치료조매천이75 mg/kg적제량관위백려호순2차,치료대조조매천이등체적무균수관위2차；균정상섭식、섭수.지속치료4개월후,각조대서경고정맥채혈2 ml,채집방수50μl.검측공복혈당、방수포도당、혈장담고순급혈장감유삼지수평.이용이범사람(EB)측정각조대서시망막혈관통투성.분리대서시망막,관찰각조대서시망막혈관중주세포수량적변화；제취총단백,채용단백질면역인적법(Western blot)검측각조대서시망막중혈관내피생장인자(VEGF)적표체.결과 지속치료4개월후,3조대서공복혈당、방수포도당、혈장총담고순급혈장감유삼지수평비교,치료대조조교정상대조조명현승고；백려호순치료조여치료대조조비교,제혈장총담고순무명현차이외,기여지표균명현강저；3조간각지표차이균유통계학의의(F=152.809、65.230、3.861、15.059,P＜0.05).3조대서시망막혈관통투성비교,치료대조조교정상대조조고,백려호순치료조교치료대조조저；3조간차이유통계학의의(F=11.626,P＜0.05).3조대서시망막혈관중주세포수량비교,치료대조조교정상대조조명현감소,백려호순치료조교치료대조조명현증가；3조간차이유통계학의의(F=43.284,P＜0.05).3조대서시망막VEGF표체수평비교,치료대조조교정상대조조명현승고,백려호순치료조교치료대조조명현강저；3조간차이유통계학의의(F=14.017,P＜0.05).결론 백려호순능통과강저공복혈당、방수포도당급혈장감유삼지수평,억제VEGF고표체이개선당뇨병시망막혈관적결구화공능이상.
Objective To observe the effect of resveratrol on retinal vasculopathy in diabetic rats.Methods Forty-five Sprague-Dawley male rats were randomly divided into the resveratrol group,treatment control group and the normal control group,15 rats in each group.Diabetic rat models were induced with streptozotocin injection in resveratrol group and treatment control group.The same volume of sterile saline solution was injected into the rats of the normal control group.The rats of resveratrol group and treatment control group were feed with high-fat diet.The rats of resveratrol group received oral gavage of resveratrol (75 mg/kg) twice a day for four months.The same volume of sterile saline solution was given by gavage in rats of treatment control group twice a day for four months.2 ml femoral vein blood and 50 μl aqueous fluid of anterior chamber of the eye from rats of three groups were collected to detect fasting blood glucose,aqueous fluid glucose,cholesterol and triglyceride.The retinal vascular permeability was test by labeling with evans blue.Whole retina was isolated to detect the pericyte number.Total protein was extracted from retina to test the level of vascular endothelial growth factor (VEGF).Results The fasting blood glucose,aqueous fluid glucose,cholesterol and triglyceride in treatment control group were higher than those in normal control group,also higher than those in resveratrol group except cholesterol.The differences among the three groups were statistically significant (F=152.809,65.230,3.861,15.059; P＜0.05).The retinal vascular permeability in treatment control group was higher than that in normal control group,while it in resveratrol group was lower than that in treatment control group.The differences among the three groups was statistically significant (F=11.626,P＜0.05).The pericyte number in treatment control group decreased as compared to normal control group,while it in resveratrol group increased as compared to treatment control group.The differences among the three groups was statistically significant (F=43.284,P＜0.05).The VEGF expression in treatment control group increased as compared to normal control group,while it in resveratrol group decreased as compared to treatment control group.The differences among the three groups was statistically significant (F=14.017,P＜0.05).Conclusion Resveratrol can improve abnormal retinal vasculopathy structure and function,down-regulated level of fasting blood glucose,aqueous fluid glucose,triglyceride and VEGF may be the mechanism.
