中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2014年
2期
166-170
,共5页
毕雪%陈松%林锦镛%王玉川
畢雪%陳鬆%林錦鏞%王玉川
필설%진송%림금용%왕옥천
糖尿病性视网膜病变/治疗%间质干细胞移植/方法%脑源性神经营养因子
糖尿病性視網膜病變/治療%間質榦細胞移植/方法%腦源性神經營養因子
당뇨병성시망막병변/치료%간질간세포이식/방법%뇌원성신경영양인자
Diabetic retinopathy/therapy%Mesenchymal stem cell transplantation/methods%Brain-derived neurotrophic factor
目的 观察人脐带间充质干细胞(hUCMSC)对糖尿病(DM)大鼠血糖的影响及对视网膜病变的治疗作用.方法 健康雄性Sprague-Dawley大鼠45只,随机分为正常对照组(A组)、DM组,分别为10、35只大鼠.DM组经尾静脉注射链脲佐菌素诱导DM模型.10周时,A组、DM组分别随机选取2只大鼠行视网膜血管铺片.DM组剩余的33只大鼠随机分为糖尿病视网膜病变组(B组)、尾静脉注射hUCMSC组(C组)、玻璃体腔注射hUCMSC组(D组),各组均为11只大鼠.A、B组不进行干预.干预后2、4、6、8周为观察处理时间点.各处理时间点前,连续3d每次随机选取2只大鼠检测随机血糖.DM组大鼠血糖与同期A组大鼠血糖比较,差异均有统计学意义(t=-64.400、-60.601、-44.065、-43.872,P=0.000).8周时从B、C、D组随机选取2只大鼠行视网膜血管铺片.免疫组织化学法观察视网膜脑源性神经营养因子(BDNF)阳性染色情况;实时荧光定量逆转录聚合酶链反应(RT-PCR)检测视网膜BDNFmRNA相对表达量.结果 干预后,不同处理时间点A、B、C、D组大鼠血糖比较,差异均有统计学意义(F=400.017、404.410、422.043、344.109,P=0.000);C组大鼠血糖与B、D组大鼠血糖比较,差异有统计学意义(t=4.447、4.990、P＜0.01).免疫组织化学染色结果显示,A组BDNF表达呈阳性,主要分布在神经节细胞层;B组BDNF表达呈弱阳性;C、D组BDNF表达增多.RT-PCR检测结果显示,4、6、8周时,B、C、D组间视网膜BDNF mRNA相对表达量比较,差异均有统计学意义(F=29.372、188.492、421.537,P=0.000);C、D组视网膜BDNF mRNA相对表达量与B组比较,差异均有统计学意义(t=66.781、72.401、63.880、88.423、75.120、83.002,P＜0.01);C组视网膜BDNF mRNA相对表达量与D组比较,仅8周时差异有统计学意义(t=127.321,P=0.005).结论 尾静脉注射hUCMSC能够显著降低大鼠血糖水平;尾静脉或玻璃体腔注射hUCMSC均可增加BDNF的表达.
目的 觀察人臍帶間充質榦細胞(hUCMSC)對糖尿病(DM)大鼠血糖的影響及對視網膜病變的治療作用.方法 健康雄性Sprague-Dawley大鼠45隻,隨機分為正常對照組(A組)、DM組,分彆為10、35隻大鼠.DM組經尾靜脈註射鏈脲佐菌素誘導DM模型.10週時,A組、DM組分彆隨機選取2隻大鼠行視網膜血管鋪片.DM組剩餘的33隻大鼠隨機分為糖尿病視網膜病變組(B組)、尾靜脈註射hUCMSC組(C組)、玻璃體腔註射hUCMSC組(D組),各組均為11隻大鼠.A、B組不進行榦預.榦預後2、4、6、8週為觀察處理時間點.各處理時間點前,連續3d每次隨機選取2隻大鼠檢測隨機血糖.DM組大鼠血糖與同期A組大鼠血糖比較,差異均有統計學意義(t=-64.400、-60.601、-44.065、-43.872,P=0.000).8週時從B、C、D組隨機選取2隻大鼠行視網膜血管鋪片.免疫組織化學法觀察視網膜腦源性神經營養因子(BDNF)暘性染色情況;實時熒光定量逆轉錄聚閤酶鏈反應(RT-PCR)檢測視網膜BDNFmRNA相對錶達量.結果 榦預後,不同處理時間點A、B、C、D組大鼠血糖比較,差異均有統計學意義(F=400.017、404.410、422.043、344.109,P=0.000);C組大鼠血糖與B、D組大鼠血糖比較,差異有統計學意義(t=4.447、4.990、P＜0.01).免疫組織化學染色結果顯示,A組BDNF錶達呈暘性,主要分佈在神經節細胞層;B組BDNF錶達呈弱暘性;C、D組BDNF錶達增多.RT-PCR檢測結果顯示,4、6、8週時,B、C、D組間視網膜BDNF mRNA相對錶達量比較,差異均有統計學意義(F=29.372、188.492、421.537,P=0.000);C、D組視網膜BDNF mRNA相對錶達量與B組比較,差異均有統計學意義(t=66.781、72.401、63.880、88.423、75.120、83.002,P＜0.01);C組視網膜BDNF mRNA相對錶達量與D組比較,僅8週時差異有統計學意義(t=127.321,P=0.005).結論 尾靜脈註射hUCMSC能夠顯著降低大鼠血糖水平;尾靜脈或玻璃體腔註射hUCMSC均可增加BDNF的錶達.
목적 관찰인제대간충질간세포(hUCMSC)대당뇨병(DM)대서혈당적영향급대시망막병변적치료작용.방법 건강웅성Sprague-Dawley대서45지,수궤분위정상대조조(A조)、DM조,분별위10、35지대서.DM조경미정맥주사련뇨좌균소유도DM모형.10주시,A조、DM조분별수궤선취2지대서행시망막혈관포편.DM조잉여적33지대서수궤분위당뇨병시망막병변조(B조)、미정맥주사hUCMSC조(C조)、파리체강주사hUCMSC조(D조),각조균위11지대서.A、B조불진행간예.간예후2、4、6、8주위관찰처리시간점.각처리시간점전,련속3d매차수궤선취2지대서검측수궤혈당.DM조대서혈당여동기A조대서혈당비교,차이균유통계학의의(t=-64.400、-60.601、-44.065、-43.872,P=0.000).8주시종B、C、D조수궤선취2지대서행시망막혈관포편.면역조직화학법관찰시망막뇌원성신경영양인자(BDNF)양성염색정황;실시형광정량역전록취합매련반응(RT-PCR)검측시망막BDNFmRNA상대표체량.결과 간예후,불동처리시간점A、B、C、D조대서혈당비교,차이균유통계학의의(F=400.017、404.410、422.043、344.109,P=0.000);C조대서혈당여B、D조대서혈당비교,차이유통계학의의(t=4.447、4.990、P＜0.01).면역조직화학염색결과현시,A조BDNF표체정양성,주요분포재신경절세포층;B조BDNF표체정약양성;C、D조BDNF표체증다.RT-PCR검측결과현시,4、6、8주시,B、C、D조간시망막BDNF mRNA상대표체량비교,차이균유통계학의의(F=29.372、188.492、421.537,P=0.000);C、D조시망막BDNF mRNA상대표체량여B조비교,차이균유통계학의의(t=66.781、72.401、63.880、88.423、75.120、83.002,P＜0.01);C조시망막BDNF mRNA상대표체량여D조비교,부8주시차이유통계학의의(t=127.321,P=0.005).결론 미정맥주사hUCMSC능구현저강저대서혈당수평;미정맥혹파리체강주사hUCMSC균가증가BDNF적표체.
Objective To observe the effects of human umbilical cord mesenchymal stem cells (hUCMSCs) on blood glucose levels and diabetic retinopathy in diabetes mellitus (DM) rats.Method A total of 45 healthy male Sprague-Dawley rats were randomly divided into normal control group (group A,10 rats) and DM group (33 rats).Diabetic model was established in DM group by tail vein injection of streptozotocin.The DM group was further randomly divided into 3 groups (11 rats in each group),including group B (no transplantation),group C (hUCMSC was injected through tail vein) and group D (hUCMSC was injected into the vitreous).Blood glucose,retina wholemont staining and expression of brain derived neurotrophic factor (BDNF) in the retina were measured at 2,4,6,8 weeks after hUCMSC injection.The blood glucose was significantly different between A-D groups before injection (t=-64.400,-60.601,-44.065,-43.872; P=0.000) BDNF expression was studied by real time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry staining.Results The blood glucose was significantly different between A-D groups after hUCMSC injection (F=400.017,404.410,422.043,344.109; P=0.000),and between group C and group B/D (t=4.447,4.990; P＜0.01).Immuno-staining shown that BDNF was positive in ganglion cell layer (RGC) of group A,weak in group B while BDNF expression increased in group C/D.BDNF mRNA expression was significantly different between group B,C andDat 4,6 and 8 weeks after hUCMSC injection (F 29.372,188.492,421.537; P=0.000),and between group B and C/D (t=66.781,72.401,63.880,88.423,75.120,83.002; P＜0.01) by RT-PCR analysis.The BDNF mRNA expression was significantly different between C and D groups only at 8 weeks after hUCMSC injection (t =127.321,P =0.005).Conclusions Tail vein injection of hUCMSCs can significantly reduce the blood glucose levels of rats.Intravenous and intravitreal injection of hUCMSCs can increase the expression of BDNF.
