CAJ | 학술논문

目的观察新型MicronⅣ视网膜影像系统检查在3种小鼠疾病模型中的应用效果.方法健康雄性C57BL/6J小鼠24只.分别建立氧诱导视网膜病变(OIR)模型组、N-甲基-N-亚硝脲(MNU)模型组、N-甲基-D-天冬氨酸(NMDA)模型组.应用MicronⅣ视网膜影像系统对3种模型组小鼠行眼底彩色照相;OIR模型组、MNU模型组小鼠行荧光素眼底血管造影(FFA)、光相干断层扫描(OCT)检查;MNU模型组、NMDA模型组小鼠行视网膜电图(ERG)检查.结果 OIR模型组小鼠眼底视网膜血管高度扩张、纡曲,走形僵直;FFA检查可见形态异常、分布紊乱的新生血管丛,荧光渗漏致玻璃体混浊;OCT检查可见视网膜前纤维血管组织及纡曲扩张血管后方粗大的阴影暗区.MNU模型组小鼠眼底视盘呈蜡黄色萎缩,视网膜无明显色素性改变;FFA检查可见视网膜血管轻微变细;OCT检查可见视网膜厚度和结构变化不明显;ERG检查结果显示,a、b波潜伏期变化不大,建模型后2、3d最大混合反应a波振幅分别为(15.38±4.36)、(13.78±5.52) μV,明显下降.NMDA模型组小鼠眼底视网膜呈苍白色改变,血管痉挛纡曲;ERG检查结果显示,a、b波潜伏期变化不大,建模型12、24 h最大混合反应b波振幅分别为(72.28±7.18)、(65.35±9.18) μV,明显下降.结论 Micron Ⅳ视网膜影像系统能实时、无创观察视网膜结构和功能.
목적 관찰신형MicronⅣ시망막영상계통검사재3충소서질병모형중적응용효과.방법 건강웅성C57BL/6J소서24지.분별건립양유도시망막병변(OIR)모형조、N-갑기-N-아초뇨(MNU)모형조、N-갑기-D-천동안산(NMDA)모형조.응용MicronⅣ시망막영상계통대3충모형조소서행안저채색조상;OIR모형조、MNU모형조소서행형광소안저혈관조영(FFA)、광상간단층소묘(OCT)검사;MNU모형조、NMDA모형조소서행시망막전도(ERG)검사.결과 OIR모형조소서안저시망막혈관고도확장、우곡,주형강직;FFA검사가견형태이상、분포문란적신생혈관총,형광삼루치파리체혼탁;OCT검사가견시망막전섬유혈관조직급우곡확장혈관후방조대적음영암구.MNU모형조소서안저시반정사황색위축,시망막무명현색소성개변;FFA검사가견시망막혈관경미변세;OCT검사가견시망막후도화결구변화불명현;ERG검사결과현시,a、b파잠복기변화불대,건모형후2、3d최대혼합반응a파진폭분별위(15.38±4.36)、(13.78±5.52) μV,명현하강.NMDA모형조소서안저시망막정창백색개변,혈관경련우곡;ERG검사결과현시,a、b파잠복기변화불대,건모형12、24 h최대혼합반응b파진폭분별위(72.28±7.18)、(65.35±9.18) μV,명현하강.결론 Micron Ⅳ시망막영상계통능실시、무창관찰시망막결구화공능.
Objective To evaluate Micron Ⅳ retinal imaging system in three mouse models of retinal diseases.Methods Mouse models of oxygen induced retinopathy (OIR) model (OIR group),N-methyl-N nitrosourea (MNU) model (MNU group) and N-methyl-D-aspartate (NMDA) model (NMDA group) were induced in 24 healthy male C57BL/6J mice.Fundus photograph,fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) and electroretinogram (ERG) were used to evaluate these mice.All the imaging examinations were performed by Micron Ⅳ retinal imaging system.Results OIR mice showed tortuous and dilated retinal vessels in fundus photograph,neovascularization plexus and vascular leakage in FFA,and epiretinal fibrovascular tissue and tortuous expansion vascular vessels in OCT.MNU mice showed wax yellow optic disk without retinal pigmentary changes,slight thinning of retinal blood vessels in FFA,and normal structure and thickness in OCT.The a-wave amplitudes of the maximum mixed response decreased significantly,and were (15.38±4.36) μV and (13.78±5.52) μV at 2 or 3 days of modeling,respectively.NMDA mice showed a pale retina with vasospasm.ERG revealed that there was no obvious change in latency of a-and b-wave,but significantly decreased amplitude of b-wave at 12 hours and 24 hours after modeling with (72.28±7.18) μV and (65.35±9.18) μV,respectively.Conclusion Micron Ⅳ retinal imaging system is a real-time,non-invasive tool to study the retinal structure and function in animal models of retinal diseases.