CAJ | 학술논문

目的探讨电压依赖性钙通道(VDCC)α1F亚单位在小鼠视网膜组织中的分布和意义.方法实验研究.将70只小鼠分为两组,实验组为VDCC α1F-/-小鼠(突变纯合子),对照组为α1F+/+小鼠(野生型),每组35只.免疫荧光法检测两组小鼠视网膜组织中α1F亚单位的表达情况；两组小鼠于生后3、6、9、14 d和3个月分别行视网膜石蜡切片观察显微结构；并记录两组小鼠闪光视网膜电图(ERG).两组小鼠ERG振幅的比较采用独立样本t检验.结果 (1)实验组小鼠视网膜组织中VDCC α1F亚单位呈阴性表达；对照组小鼠视网膜组织中VDCC α1F亚单位呈阳性表达,主要分布于视网膜外丛状层(OPL),内丛状层和神经节细胞层可见少量表达.(2)α1F-/-小鼠出生后视网膜组织中的OPL逐渐变窄,至3个月龄时几乎消失；而α1F+/+小鼠视网膜OPL厚度始终变化不大.(3)α1F-/-小鼠暗适应标准强度闪光ERG b波振幅[(163.8±26.7)μV]较α1F+/+小鼠[(408.4±54.5)μV]明显降低(t=-9.017,P=0.000),而α1F-/-小鼠a波振幅[(208.2 ±27.3) μV]与α1F+/+小鼠[(196.0 ±24.2)μV]的差异无统计学意义(t=0.748,P=0.476).结论 VDCCα1F亚单位缺陷主要影响小鼠视网膜OPL的结构和功能.
목적 탐토전압의뢰성개통도(VDCC)α1F아단위재소서시망막조직중적분포화의의.방법 실험연구.장70지소서분위량조,실험조위VDCC α1F-/-소서(돌변순합자),대조조위α1F+/+소서(야생형),매조35지.면역형광법검측량조소서시망막조직중α1F아단위적표체정황；량조소서우생후3、6、9、14 d화3개월분별행시망막석사절편관찰현미결구；병기록량조소서섬광시망막전도(ERG).량조소서ERG진폭적비교채용독립양본t검험.결과 (1)실험조소서시망막조직중VDCC α1F아단위정음성표체；대조조소서시망막조직중VDCC α1F아단위정양성표체,주요분포우시망막외총상층(OPL),내총상층화신경절세포층가견소량표체.(2)α1F-/-소서출생후시망막조직중적OPL축점변착,지3개월령시궤호소실；이α1F+/+소서시망막OPL후도시종변화불대.(3)α1F-/-소서암괄응표준강도섬광ERG b파진폭[(163.8±26.7)μV]교α1F+/+소서[(408.4±54.5)μV]명현강저(t=-9.017,P=0.000),이α1F-/-소서a파진폭[(208.2 ±27.3) μV]여α1F+/+소서[(196.0 ±24.2)μV]적차이무통계학의의(t=0.748,P=0.476).결론 VDCCα1F아단위결함주요영향소서시망막OPL적결구화공능.
Objective To investigate the distribution and biological roles of voltage-dependent calcium channel (VDCC) α1F subunit in murine retina.Methods Experimental study.α1 F-/-(homozygous mutant) mice (n =35) and α1 F +/+ (wild type) mice (n =35) were used in this study.Immunohistochemistry was performed to determine the expression of VDCC α1F subunit in the mouse retina.Retinae in α1 F-/-mice and age-matched control mice at 3,6,9,14-day and 3-month after birth were paraffin embedded,sectioned and HE stained,and full-field electroretinogram (ERG) were also recorded at these time points.Statistics were based on independent samples t-test.Results (1) α1 F subunit was absent in o1F-/-mice retina.But in α1F+/+ mice retina,α1F subunit was expressed most strongly in the outer plexiform layer (OPL),less in the inner plexiform layer (IPL) and ganglion cell layer (GCL).(2) OPL thickness in the subunit deficient mice gradually reduced after birth and lost at adult age.(3) In darkadapted E RGs,standard response showed that the b-wave amplitude of α1 F-/-mice [(163.8 ± 26.7) μV]significantly decreased compared with that of α1 F +/+ mice [(408.4 ± 54.5) μV] (t =-9.017,P =0.000),whereas the a-wave amplitude ofα1F-/-group [(208.2 ±27.3) μV] was similar to that of control group [(196.0 ±24.2) μV] (t =0.748,P =0.476).Conclusion This study demonstrates that the lack of VDCC α1 F subunit affect the structure and function in the OPL of the murine retina.