CAJ | 학술논문

目的探讨激光诱导的恒河猴CNV模型的形态学和功能学变化.方法实验研究.8只体重4～7 kg的成年恒河猴用于该研究.采用高能量、短曝光时间和小光斑直径的氩激光光凝视网膜诱导CNV.通过彩色眼底拍照、FFA和OCT等方法对恒河猴CNV的自然病程进行连续观测.采用单因素重复测量设计资料的方差分析法对FFA检查的4级光斑荧光素渗漏强度进行统计分析,同时采用配对t检验的方法对激光建模前及激光光凝建模后56 d恒河猴CNV模型视觉电生理各参数的改变进行统计分析.结果 (1)FFA检查结果显示激光光凝后14、21、28、35、42、49、56 d的4级光斑平均荧光素渗漏强度的灰度值分别为89.44±26.28、97.56±26.47、110.22±29.76、100.26±29.24、91.77±28.11、77.76±24.85、63.23±22.34,差异有统计学意义(F =39.715,P ＜0.01).每个时间点的荧光素渗漏强度与前一个时间点的荧光素渗漏强度相比,差异均有统计学意义(t14～21=4.824,P＜0.01; t21～28=5.225,P＜0.01; t28-35=7.378,P＜0.01;t35 ～42=2.954,P＜0.05; t42～49=5.386,P＜0.01;t49～56=6.138,P＜0.01).(2)OCT检查结果可见光斑处有代表CNV形成的红色高反射光团,并伴有视网膜水肿及视网膜下积液.组织学HE染色检查结果证实CNV形成并可见较多色素细胞增殖、移行至CNV边界.(3)视网膜电图检查数据显示,与建模前相比,激光建模后56 d暗适应b波潜伏期延长(=4.23,P＜O.01),暗适应a波振幅(t=6.35,P＜0.01)、暗适应b波振幅(t=3.12,P＜0.01)及明适应b波振幅(t=3.93,P＜0.01)均下降,差异有统计学意义.结论激光诱导的恒河猴CNV模型同时具备持续荧光素渗漏的形态学特点和潜伏期延长、振幅下降的功能学改变特点,可用于CNV发病机制的研究及CNV治疗药物有效性的筛选.
목적 탐토격광유도적항하후CNV모형적형태학화공능학변화.방법 실험연구.8지체중4～7 kg적성년항하후용우해연구.채용고능량、단폭광시간화소광반직경적아격광광응시망막유도CNV.통과채색안저박조、FFA화OCT등방법대항하후CNV적자연병정진행련속관측.채용단인소중복측량설계자료적방차분석법대FFA검사적4급광반형광소삼루강도진행통계분석,동시채용배대t검험적방법대격광건모전급격광광응건모후56 d항하후CNV모형시각전생리각삼수적개변진행통계분석.결과 (1)FFA검사결과현시격광광응후14、21、28、35、42、49、56 d적4급광반평균형광소삼루강도적회도치분별위89.44±26.28、97.56±26.47、110.22±29.76、100.26±29.24、91.77±28.11、77.76±24.85、63.23±22.34,차이유통계학의의(F =39.715,P ＜0.01).매개시간점적형광소삼루강도여전일개시간점적형광소삼루강도상비,차이균유통계학의의(t14～21=4.824,P＜0.01; t21～28=5.225,P＜0.01; t28-35=7.378,P＜0.01;t35 ～42=2.954,P＜0.05; t42～49=5.386,P＜0.01;t49～56=6.138,P＜0.01).(2)OCT검사결과가견광반처유대표CNV형성적홍색고반사광단,병반유시망막수종급시망막하적액.조직학HE염색검사결과증실CNV형성병가견교다색소세포증식、이행지CNV변계.(3)시망막전도검사수거현시,여건모전상비,격광건모후56 d암괄응b파잠복기연장(=4.23,P＜O.01),암괄응a파진폭(t=6.35,P＜0.01)、암괄응b파진폭(t=3.12,P＜0.01)급명괄응b파진폭(t=3.93,P＜0.01)균하강,차이유통계학의의.결론 격광유도적항하후CNV모형동시구비지속형광소삼루적형태학특점화잠복기연장、진폭하강적공능학개변특점,가용우CNV발병궤제적연구급CNV치료약물유효성적사선.
Objective To investigate the morphological and functional changes of the laser-induced choroidal neovascularization(CNV) in rhesus monkeys.Methods Experimental study.Eight adult rhesus monkeys weighted 4 to 7 kg were used in this study.CNVs were induced with small high-energy laser spots at short pulse duration by an argon green laser.Eyes were monitored weekly by color fundus photography,fluorescence fundus angiography (FFA),and optical coherence tomography (OCT).Fluorescein leaking intensities of grade 4 CNV lesions were analyzed by the method of ANOVA for repeated measures.Electroretinogram(ERG) was performed before laser photocoagulation and 56 days after laser photocoagulation and the data were analyzed with paired t-test.Results (1) FFA revealed that the mean intensities of grade 4 CNV lesions were 89.44 ± 26.28,97.56 ± 26.47,110.22 ± 29.76,100.26 ± 29.24,91.77 ± 28.11,77.76±24.85 and 63.23 ±22.34 on day 14,day 21,day 28,day 35,day 42,day 49,and day 56 respectively and the differences were statistically significant(F =39.715,P ＜ 0.01).The differences between any time-point and its previous time-point were also statistically significant (t14-21 =4.824,P ＜ 0.01 ;t21-28 =5.225,P ＜ 0.01 ;t28-35 =7.378,P ＜ 0.01 ; t35-42 =2.954,P ＜ 0.05 ; t42-49 =5.386,P ＜ 0.01 ; t49-56 =6.138,P ＜0.01).(2)OCT images showed retinal edema,subretinal fluid and hyper-reflective lesions of CNVs inthe laser sites and histopathology showed that fibrovascular tissues together with proliferating retinal pigment epithelium cells were seen in the laser sites.(3) ERG data revealed that implicit time of dark-adapted b wave (t =4.23,P ＜ 0.01) increased while the amplitudes of dark-adapted a wave (t =6.35,P ＜ 0.01),darkadapted b wave(t =3.12,P ＜ 0.01) and light-adapted b wave (t =3.93,P ＜ 0.01) decreased 56 days after laser photocoagulation compared with those before laser photocoagulation.Conelusion The laser-induced CNV in non-human primate model shows continuous leakage on FFA examination and reduced amplitudes as well as increased implicit time on ERG examination,suggesting that laser-induced CNV primate model not only could be used to study the pathogenesis of CNV formation but also could be used for screening of drug effectiveness.