中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2014年
4期
273-276
,共4页
角膜疾病%内皮,角膜%细胞移植%冷冻%疾病模型,动物
角膜疾病%內皮,角膜%細胞移植%冷凍%疾病模型,動物
각막질병%내피,각막%세포이식%냉동%질병모형,동물
Corneal diseases%Endothelium,corneal%Cell transplantation%Freezing%Disease models,animal
目的 探讨冷冻法构建大鼠角膜内皮功能不全模型的可行性及B4G12细胞的生物安全性和泵功能.方法 实验研究.用随机数字表法将30只SD大鼠随机分为单纯冷冻组及冷冻+B4G12细胞移植组;液氮冷冻法制备角膜内皮功能不全模型,前房注射法将B4G12细胞移植到模型动物眼内.术后定期对各组动物进行眼前节观察、激光共聚焦显微镜检察以及病理学检查.结果 冷冻后角膜内皮细胞被完全去除,单纯冷冻组大鼠角膜水肿、混浊,B4G12细胞移植组术后2周角膜均恢复透明,厚度与正常角膜接近.激光共聚焦显微镜及病理染色证实单纯冷冻组后弹力层裸露,基质水肿;细胞移植组内皮细胞贴附良好,内皮完整.结论 液氮冷冻法可用于制备大鼠角膜内皮功能不全模型,B4G12细胞移植后短期不引起异常新生物及炎症反应,具有内皮泵功能.
目的 探討冷凍法構建大鼠角膜內皮功能不全模型的可行性及B4G12細胞的生物安全性和泵功能.方法 實驗研究.用隨機數字錶法將30隻SD大鼠隨機分為單純冷凍組及冷凍+B4G12細胞移植組;液氮冷凍法製備角膜內皮功能不全模型,前房註射法將B4G12細胞移植到模型動物眼內.術後定期對各組動物進行眼前節觀察、激光共聚焦顯微鏡檢察以及病理學檢查.結果 冷凍後角膜內皮細胞被完全去除,單純冷凍組大鼠角膜水腫、混濁,B4G12細胞移植組術後2週角膜均恢複透明,厚度與正常角膜接近.激光共聚焦顯微鏡及病理染色證實單純冷凍組後彈力層裸露,基質水腫;細胞移植組內皮細胞貼附良好,內皮完整.結論 液氮冷凍法可用于製備大鼠角膜內皮功能不全模型,B4G12細胞移植後短期不引起異常新生物及炎癥反應,具有內皮泵功能.
목적 탐토냉동법구건대서각막내피공능불전모형적가행성급B4G12세포적생물안전성화빙공능.방법 실험연구.용수궤수자표법장30지SD대서수궤분위단순냉동조급냉동+B4G12세포이식조;액담냉동법제비각막내피공능불전모형,전방주사법장B4G12세포이식도모형동물안내.술후정기대각조동물진행안전절관찰、격광공취초현미경검찰이급병이학검사.결과 냉동후각막내피세포피완전거제,단순냉동조대서각막수종、혼탁,B4G12세포이식조술후2주각막균회복투명,후도여정상각막접근.격광공취초현미경급병리염색증실단순냉동조후탄력층라로,기질수종;세포이식조내피세포첩부량호,내피완정.결론 액담냉동법가용우제비대서각막내피공능불전모형,B4G12세포이식후단기불인기이상신생물급염증반응,구유내피빙공능.
Objective To set up an animal model of corneal endothelium deficiency and test the bio-safety and pump function of B4G12 cells with this model.Methods Thirty SD rats were divided into cryo-injury group and cryo-injury with B4G12 cell transplantation group.Models of corneal endothelium deficiency were created by cryo-injury with liquid nitrogen,and then B4G12 cells were transplanted into the eyes by anterior chamber injection.Corneas were checked under slit lamp and confocal microscope observations at some specific time points,pathological staining was also performed.Results The corneal endothelial cells were removed completely from the Descemet's membrane after cryo-injury.Cells transplanted firmly stuck to the Descemet's membrane.The corneas in the cryo-injury group were swelling with haze,while in the transplantation groups,the corneas restored transparence and normal thickness 2 weeks after the surgery.Confocal microscope and HE staining confirmed that in the cryo-injury group,the Descemet's membrane was denuded and the cornea stroma layer was in edema,on the contrary,in the other group,the transplanted cells completely covered the Descemet's membrane.Conclusion Cryo-injury can be used for building the rat model of corneal endothelium deficiency,no neoplasm and inflammation reaction were found during the observation after B4G12 cells transplanted and B4G12 cells had the pump function in vivo.
