中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2014年
7期
511-517
,共7页
王靖瑞%史学锋%张伟%赵堪兴
王靖瑞%史學鋒%張偉%趙堪興
왕정서%사학봉%장위%조감흥
视觉诱发电位%大鼠%空间频率%时间频率
視覺誘髮電位%大鼠%空間頻率%時間頻率
시각유발전위%대서%공간빈솔%시간빈솔
Visual evoked potentials%Rats%Spatial frequency%Temporal frequency
目的 利用图形视觉诱发电位(PVEP)观察Wistar大鼠视皮层对不同空间和时间频率视觉刺激的反应调谐特性.方法 实验研究.8周龄Wistar大鼠15只,麻醉后于初级视皮层对应位置开颅并于硬脑膜表面慢性埋置记录电极,采用同样方法于鼻骨处埋置参考电极.分别于术后第3、5、7、9天行PVEP检测.采用图形棋盘格刺激,空间频率分别为0.01、0.02、0.04、0.08 cpd,时间频率分别为l、2、4、6 Hz.采用Matlab及SPSS软件对数据进行处理和统计学分析,比较不同时间、空间频率对大鼠PVEP幅度和潜伏期的影响,以及测量值的个体间变异和重复测量的变异性.结果 0.01、0.02、0.04、0.08 cpd空间频率下,P100波振幅分别为(29.87±10.37)、(31.92±10.98)、(28.46±6.18)、(20.71 ±6.54) μV,差异有统计学意义(F=3.725,P=0.018);P100波潜伏期分别为(96.25±12.12)、(95.73±15.13)、(101.75±8.11)、(125.58±18.32) ms,差异有统计学意义(F=12.187,P =0.000).1、2、4、6 Hz时间频率下P100波振幅分别为(30.71 ±8.25)、(29.75±4.76)、(25.79±8.51)、(19.63±6.00) μV,差异有统计学意义(F=6.115,P=0.001);P100波潜伏期分别为(102.58±16.09)、(101.75±10.32)、(104.25±12.51)、(102.67±13.59)ms,差异无统计学意义(F=0.074,P =0.974).空间频率为0.04cpd,时间频率为2 Hz时,PVEP的P100波振幅和潜伏期的个体间变异系数最小,而各种刺激条件下重复性测量的变异系数总体上无显著改变.结论 在适宜刺激和记录条件下可获得Wistar大鼠稳定可靠的PVEP记录.Wistar大鼠PVEP的P100波振幅的空间频率、时间频率调谐均表现为低通特性.空间频率对Wistar大鼠PVEP的P100波潜伏期的影响较为显著,而时间频率不影响PVEP的P100波潜伏期.
目的 利用圖形視覺誘髮電位(PVEP)觀察Wistar大鼠視皮層對不同空間和時間頻率視覺刺激的反應調諧特性.方法 實驗研究.8週齡Wistar大鼠15隻,痳醉後于初級視皮層對應位置開顱併于硬腦膜錶麵慢性埋置記錄電極,採用同樣方法于鼻骨處埋置參攷電極.分彆于術後第3、5、7、9天行PVEP檢測.採用圖形棋盤格刺激,空間頻率分彆為0.01、0.02、0.04、0.08 cpd,時間頻率分彆為l、2、4、6 Hz.採用Matlab及SPSS軟件對數據進行處理和統計學分析,比較不同時間、空間頻率對大鼠PVEP幅度和潛伏期的影響,以及測量值的箇體間變異和重複測量的變異性.結果 0.01、0.02、0.04、0.08 cpd空間頻率下,P100波振幅分彆為(29.87±10.37)、(31.92±10.98)、(28.46±6.18)、(20.71 ±6.54) μV,差異有統計學意義(F=3.725,P=0.018);P100波潛伏期分彆為(96.25±12.12)、(95.73±15.13)、(101.75±8.11)、(125.58±18.32) ms,差異有統計學意義(F=12.187,P =0.000).1、2、4、6 Hz時間頻率下P100波振幅分彆為(30.71 ±8.25)、(29.75±4.76)、(25.79±8.51)、(19.63±6.00) μV,差異有統計學意義(F=6.115,P=0.001);P100波潛伏期分彆為(102.58±16.09)、(101.75±10.32)、(104.25±12.51)、(102.67±13.59)ms,差異無統計學意義(F=0.074,P =0.974).空間頻率為0.04cpd,時間頻率為2 Hz時,PVEP的P100波振幅和潛伏期的箇體間變異繫數最小,而各種刺激條件下重複性測量的變異繫數總體上無顯著改變.結論 在適宜刺激和記錄條件下可穫得Wistar大鼠穩定可靠的PVEP記錄.Wistar大鼠PVEP的P100波振幅的空間頻率、時間頻率調諧均錶現為低通特性.空間頻率對Wistar大鼠PVEP的P100波潛伏期的影響較為顯著,而時間頻率不影響PVEP的P100波潛伏期.
목적 이용도형시각유발전위(PVEP)관찰Wistar대서시피층대불동공간화시간빈솔시각자격적반응조해특성.방법 실험연구.8주령Wistar대서15지,마취후우초급시피층대응위치개로병우경뇌막표면만성매치기록전겁,채용동양방법우비골처매치삼고전겁.분별우술후제3、5、7、9천행PVEP검측.채용도형기반격자격,공간빈솔분별위0.01、0.02、0.04、0.08 cpd,시간빈솔분별위l、2、4、6 Hz.채용Matlab급SPSS연건대수거진행처리화통계학분석,비교불동시간、공간빈솔대대서PVEP폭도화잠복기적영향,이급측량치적개체간변이화중복측량적변이성.결과 0.01、0.02、0.04、0.08 cpd공간빈솔하,P100파진폭분별위(29.87±10.37)、(31.92±10.98)、(28.46±6.18)、(20.71 ±6.54) μV,차이유통계학의의(F=3.725,P=0.018);P100파잠복기분별위(96.25±12.12)、(95.73±15.13)、(101.75±8.11)、(125.58±18.32) ms,차이유통계학의의(F=12.187,P =0.000).1、2、4、6 Hz시간빈솔하P100파진폭분별위(30.71 ±8.25)、(29.75±4.76)、(25.79±8.51)、(19.63±6.00) μV,차이유통계학의의(F=6.115,P=0.001);P100파잠복기분별위(102.58±16.09)、(101.75±10.32)、(104.25±12.51)、(102.67±13.59)ms,차이무통계학의의(F=0.074,P =0.974).공간빈솔위0.04cpd,시간빈솔위2 Hz시,PVEP적P100파진폭화잠복기적개체간변이계수최소,이각충자격조건하중복성측량적변이계수총체상무현저개변.결론 재괄의자격화기록조건하가획득Wistar대서은정가고적PVEP기록.Wistar대서PVEP적P100파진폭적공간빈솔、시간빈솔조해균표현위저통특성.공간빈솔대Wistar대서PVEP적P100파잠복기적영향교위현저,이시간빈솔불영향PVEP적P100파잠복기.
Objective To observe the spatiotemporal frequency tuning of pattern visual evoked potentials in Wistar rats.Methods Experimental study.15 Wistar rats of 8 weeks old were used in this study.Recording electrodes were chronically inserted into the skull without piercing the dura at the corresponding sites to the primary visual cortex under anesthesia.Reference electrodes were inserted into the nose bone in the same way.Pattern visual evoked potentials were recorded on the 3rd,5th,7th and 9th postoperation days.The visual stimuli were chessboard patterns with spatial frequency of 0.01,0.02,0.04 or 0.08 cpd,and temporal frequency of 1,2,4 or 6 Hz,respectively.Data were processed and statistically analyzed with Matlab and SPSS.The amplitudes and latencies of pattern visual evoked potentials under different spatial and temporal frequencies,the inter-individual variations and the repeated measurement variations were compared.Results With the increase of spatial frequencies,the P100 amplitudes of PVEPs were gradually reduced,which were (29.87 ±10.37) μV (0.01 cpd),(31.92 ±10.98) μV (0.02 cpd),(28.46 ±6.18) μV (0.04 cpd) and (20.71 ±6.54) μV(0.08 cpd),respectively,with significant difference among groups (F =3.725,P =0.018),and the P100 latencies of PVEPs were gradually elongated,which were (96.25 ± 12.12) ms (0.01 cpd),(95.73 ± 15.13) ms (0.02 cpd),(101.75 ± 8.11) ms (0.04 cpd) and (125.58 ± 18.32) ms (0.08 cpd),respectively,with significant difference among groups (F =12.187,P =0.000).With the increase of temporal frequencies,the P100 amplitudes of PVEPs were gradually reduced,which were (30.71 ± 8.25) μV (1 Hz),(29.75 ± 4.76) μV (2 Hz),(25.79 ± 8.51) μV (4 Hz) and (19.63 ± 6.00) μV (6 Hz),with significant difference among groups (F =6.115,P =0.001),and there was no change of P100 latencies,which were (102.58 ± 16.09) ms (1 Hz),(101.75 ± 10.32) ms (2 Hz),(104.25 ±12.51) ms (4 Hz) and (102.67± 13.59) ms (6 Hz),respectively,with no significant difference among groups (F =0.074,P =0.974).The inter-individual variation was the smallest under the spatial frequency of 0.04 cpd and temporal frequency of 2 Hz.The repeated measurement variations showed no significant change under tested conditions.Conclusion Under appropriate stimuli and recording conditions,PVEP can be reliably recorded in Wistar rats.The spatiotemporal frequency tuning of P100 amplitudes of Wistar rats exhibit low-pass selectivity.The spatial frequency affects the P100 latency in Wistar rat,but not so does the temporal frequency.
