中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2014年
11期
820-825
,共6页
视网膜脱离%脑源性神经营养因子%视网膜神经节细胞%细胞凋亡%大鼠
視網膜脫離%腦源性神經營養因子%視網膜神經節細胞%細胞凋亡%大鼠
시망막탈리%뇌원성신경영양인자%시망막신경절세포%세포조망%대서
Retinal detachment%Brain-derived neurotrophic factor%Retinal ganglion cells%Apoptosis%Rats
目的 探讨实验性视网膜脱离(RD)复位后外源性脑源性神经营养因子(BDNF)对RD损伤后视网膜修复的影响.方法 实验研究.选用健康SD大鼠48只,随机分为BDNF实验组、实验对照组、RD复位组、正常对照组.采用光镜和电镜观察RD复位后视网膜形态和视网膜细胞超微结构,检测BDNF对RD复位后视网膜变性的影响;采用原位缺口末端转移酶标记法(TUNEL)检测BDNF对RD复位后视网膜细胞凋亡的影响.组间均数比较采用方差分析,进一步两两比较采用t检验.结果 视网膜自动复位平均时间为(31.3±3.5)d.光镜、电镜观察BDNF实验组与实验对照组相比,视网膜内外节损伤轻,内、外核层细胞数多,外核层厚,视网膜结构和层次排列好.BDNF实验组和实验对照组内核层厚度分别为(21.166±3.087)和(16.084±2.928) μm,外核层厚度分别为(23.508±3.679)和(12.885±3.070) μm,两组差异均有统计学意义(t=3.776,6.996;P＜0.01).BDNF实验组视网膜外核层凋亡细胞数少于实验对照组.结论 RD复位后视网膜形态及视网膜细胞超微结构发生了改变,视网膜细胞凋亡.外源性BDNF能减轻RD复位后视网膜细胞的变性及凋亡,对PD复位后视网膜损伤有一定的保护作用.
目的 探討實驗性視網膜脫離(RD)複位後外源性腦源性神經營養因子(BDNF)對RD損傷後視網膜脩複的影響.方法 實驗研究.選用健康SD大鼠48隻,隨機分為BDNF實驗組、實驗對照組、RD複位組、正常對照組.採用光鏡和電鏡觀察RD複位後視網膜形態和視網膜細胞超微結構,檢測BDNF對RD複位後視網膜變性的影響;採用原位缺口末耑轉移酶標記法(TUNEL)檢測BDNF對RD複位後視網膜細胞凋亡的影響.組間均數比較採用方差分析,進一步兩兩比較採用t檢驗.結果 視網膜自動複位平均時間為(31.3±3.5)d.光鏡、電鏡觀察BDNF實驗組與實驗對照組相比,視網膜內外節損傷輕,內、外覈層細胞數多,外覈層厚,視網膜結構和層次排列好.BDNF實驗組和實驗對照組內覈層厚度分彆為(21.166±3.087)和(16.084±2.928) μm,外覈層厚度分彆為(23.508±3.679)和(12.885±3.070) μm,兩組差異均有統計學意義(t=3.776,6.996;P＜0.01).BDNF實驗組視網膜外覈層凋亡細胞數少于實驗對照組.結論 RD複位後視網膜形態及視網膜細胞超微結構髮生瞭改變,視網膜細胞凋亡.外源性BDNF能減輕RD複位後視網膜細胞的變性及凋亡,對PD複位後視網膜損傷有一定的保護作用.
목적 탐토실험성시망막탈리(RD)복위후외원성뇌원성신경영양인자(BDNF)대RD손상후시망막수복적영향.방법 실험연구.선용건강SD대서48지,수궤분위BDNF실험조、실험대조조、RD복위조、정상대조조.채용광경화전경관찰RD복위후시망막형태화시망막세포초미결구,검측BDNF대RD복위후시망막변성적영향;채용원위결구말단전이매표기법(TUNEL)검측BDNF대RD복위후시망막세포조망적영향.조간균수비교채용방차분석,진일보량량비교채용t검험.결과 시망막자동복위평균시간위(31.3±3.5)d.광경、전경관찰BDNF실험조여실험대조조상비,시망막내외절손상경,내、외핵층세포수다,외핵층후,시망막결구화층차배렬호.BDNF실험조화실험대조조내핵층후도분별위(21.166±3.087)화(16.084±2.928) μm,외핵층후도분별위(23.508±3.679)화(12.885±3.070) μm,량조차이균유통계학의의(t=3.776,6.996;P＜0.01).BDNF실험조시망막외핵층조망세포수소우실험대조조.결론 RD복위후시망막형태급시망막세포초미결구발생료개변,시망막세포조망.외원성BDNF능감경RD복위후시망막세포적변성급조망,대PD복위후시망막손상유일정적보호작용.
Objective To investigate the effect of exogenous brain-derived neurotrophic factor (BDNF) on the retinal repair after experimental retinal detachment (RD) reattached.Methods Experimental study.Forty-eight normal rats were randomly divided into four groups:BDNF group,control group,RD reattached group and normal group.In order to detect the effects of BDNF on retinal degeneration caused by RD,the morphology of retina and the ultrastructure of retinal cells were observed by light microscopy and electron microscopy.The effect of BDNF on the apoptosis of retinal cells after RD reattached was detected by Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) technique.Data were analyzed using one-way analysis of variance (ANOVA) and secondary analysis for significance with independent-samples t-test.Results The average automatically reattached time was (31.3 ± 3.5)days.Compared with control group,there were less damage in out segment and inner segment,more retinal cells in outer nuclear layer (ONL) and inner nuclear layer (INL),thicker ONL of retina and better organizational structure of retina in BDNF group.The average thickness of ONL and INL of retina were (21.166 ± 3.087) μm,(23.508 ± 3.679) μm respectively in BDNF group,and (16.084 ± 2.928)μm,(12.885±3.070) μm respectively in control group.The thickness of ONL and INL of retina showed a significant difference in BDNF group compared with control (P ＜ 0.01).There were less TUNEL-positive cells in ONL of retina in BDNF group compared with control.Conclusions This study indicates that the morphology of retina and the ultrastructure of retinal cells have changed,and the apoptosis of retinal cells are present after RD reattached.Exogenous BDNF can reduce retinal cells degeneration and inhibit the apoptosis of retinal cells and have a certain protection effects on the retinal damage caused by RD.
