CAJ | 학술논문

目的观察微小RNA-155(miRNA-155)在肝细胞癌中的表达及其对肝癌肝移植术后肿瘤复发和患者预后的影响.方法应用定量反转录PCR检测100例肝癌样本miRNA-155表达；Kaplan-Meier和Cox比例风险回归模型分析miRNA-155对肝癌复发和预后的影响.结果肝癌肝移植术后肿瘤复发的原发肝癌45例miRNA-155表达高于55例未复发者(14.94比4.70,P=0.001)；高miRNA-155表达与肝癌微血管侵犯密切相关(P=0.001)；miRNA-155表达水平越高,患者无瘤生存期(P ＜0.001)和生存期(P ＜0.001)越短；Cox比例风险回归模型分析提示miRNA-155可作为肝癌肝移植术后独立的预后判断因素.结论 miRNA-155在肿瘤复发的原发肝癌中过表达,可作为肝癌肝移植术预后判断的分子标志物.
목적 관찰미소RNA-155(miRNA-155)재간세포암중적표체급기대간암간이식술후종류복발화환자예후적영향.방법 응용정량반전록PCR검측100례간암양본miRNA-155표체；Kaplan-Meier화Cox비례풍험회귀모형분석miRNA-155대간암복발화예후적영향.결과 간암간이식술후종류복발적원발간암45례miRNA-155표체고우55례미복발자(14.94비4.70,P=0.001)；고miRNA-155표체여간암미혈관침범밀절상관(P=0.001)；miRNA-155표체수평월고,환자무류생존기(P ＜0.001)화생존기(P ＜0.001)월단；Cox비례풍험회귀모형분석제시miRNA-155가작위간암간이식술후독립적예후판단인소.결론 miRNA-155재종류복발적원발간암중과표체,가작위간암간이식술예후판단적분자표지물.
Objective To explore the expression of microRNA-155 in hepatocellular carcinoma (HCC) and its contribution to recurrence and prognosis of HCC after liver transplantation (LT).Methods The expression levels of microRNA-155 in 100 HCC samples were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).Kaplan-Meier and Cox proportional regression analyses were utilized to determine the association of microRNA-155 expression with patient survivals.Results The expression levels of microRNA-155 were higher in primary HCC patients with post-LT recurrence (n =45,mean relative level =14.94) than those with non-recurrence (n =55,mean relative level =4.70) (P =0.001) and correlated with micro-vascular invasion of HCC tissue samples (P =0.001).The patients with a higher expression of microRNA-155 had significantly worse recurrence-free survival (RFS:(21.5 ± 3.2)months,log rank P ＜ 0.001) and overall survival (OS:(29.3 ± 3.2) months,log rank P ＜ 0.001) than those with a lower expression of microRNA-155 (RFS:(50.8 ± 3.2) months ; OS:(54.6 ± 3.5) months).Multivariate analysis revealed that a high expression of miR-155 was an independent prognostic predictor.Conclusion MicroRNA-155 is over-expressed in primary HCC with tumor recurrence and may serve as a novel biomarker for tumor recurrence and survival of HCC patients after LT.The detection of microRNA-155is of clinical significance in HCC.