中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
12期
892-896
,共5页
董志珍%姚敏%钱静%严晓娣%陈洁%严美娟%姚宁华%姚登福
董誌珍%姚敏%錢靜%嚴曉娣%陳潔%嚴美娟%姚寧華%姚登福
동지진%요민%전정%엄효제%진길%엄미연%요저화%요등복
癌,肝细胞%胰岛素样生长因子Ⅱ%RNA,小分子干扰%凋亡
癌,肝細胞%胰島素樣生長因子Ⅱ%RNA,小分子榦擾%凋亡
암,간세포%이도소양생장인자Ⅱ%RNA,소분자간우%조망
Carcinoma,hepatocellular%Insulin-like growth factorlⅡ%RNA,small interference%Apoptosis
目的 观察肝癌组织及血胰岛素样生长因子(IGF)-Ⅱ表达与病理学特征的关系,以及小于扰RNA干预IGF-ⅡmRNA转录,对HepG2细胞的影响.方法 以自身配对法收集南通大学附属医院2009年12月-2010年8月肝癌、癌周和远癌组织,分析IGF-Ⅱ表达,肝病患者血IGF-Ⅱ浓度和病理学特征；以siRNA转染HepG2细胞,干预IGF-ⅡmRNA转录,以反转录PCR、定量PCR和ELISA法,分别从转录及蛋白水平分析；以EdU和Hoechst33342染色分析HepG2细胞增殖与凋亡.结果 肝癌组织IGF-Ⅱ基因(100％,30/30)和蛋白(83.3％,25/30)高表达,均明显高于癌周(46.7％,53.3％)及远癌组织(0,0)(均P＜0.01)；肝癌患者血IGF-Ⅱ水平(3.74±0.67)ng/L明显高于肝硬化(3.10±0.63) ng/L、慢性肝炎(1.93 ±0.17) ng/L及健康对照(1.14 ±0.14) ng/L(P＜0.001),与性别、年龄、肿瘤大小和AFP浓度间未见明显相关,但与HBV感染有关(t=5.390,P＜0.001).体外研究显示IGF-Ⅱ siRNA成功转染HepG2细胞后,IGF-Ⅱ表达在转录和蛋白水平上明显受抑,呈剂量和时间依赖；抑制IGF-Ⅱ mRNA转录,HepG2细胞凋亡和对阿霉素敏感性均明显增加.结论 IGF-Ⅱ表达与HCC进展密切相关,干预其mRNA转录可促进癌细胞凋亡,增强化疗药物敏感性.
目的 觀察肝癌組織及血胰島素樣生長因子(IGF)-Ⅱ錶達與病理學特徵的關繫,以及小于擾RNA榦預IGF-ⅡmRNA轉錄,對HepG2細胞的影響.方法 以自身配對法收集南通大學附屬醫院2009年12月-2010年8月肝癌、癌週和遠癌組織,分析IGF-Ⅱ錶達,肝病患者血IGF-Ⅱ濃度和病理學特徵；以siRNA轉染HepG2細胞,榦預IGF-ⅡmRNA轉錄,以反轉錄PCR、定量PCR和ELISA法,分彆從轉錄及蛋白水平分析；以EdU和Hoechst33342染色分析HepG2細胞增殖與凋亡.結果 肝癌組織IGF-Ⅱ基因(100％,30/30)和蛋白(83.3％,25/30)高錶達,均明顯高于癌週(46.7％,53.3％)及遠癌組織(0,0)(均P＜0.01)；肝癌患者血IGF-Ⅱ水平(3.74±0.67)ng/L明顯高于肝硬化(3.10±0.63) ng/L、慢性肝炎(1.93 ±0.17) ng/L及健康對照(1.14 ±0.14) ng/L(P＜0.001),與性彆、年齡、腫瘤大小和AFP濃度間未見明顯相關,但與HBV感染有關(t=5.390,P＜0.001).體外研究顯示IGF-Ⅱ siRNA成功轉染HepG2細胞後,IGF-Ⅱ錶達在轉錄和蛋白水平上明顯受抑,呈劑量和時間依賴；抑製IGF-Ⅱ mRNA轉錄,HepG2細胞凋亡和對阿黴素敏感性均明顯增加.結論 IGF-Ⅱ錶達與HCC進展密切相關,榦預其mRNA轉錄可促進癌細胞凋亡,增彊化療藥物敏感性.
목적 관찰간암조직급혈이도소양생장인자(IGF)-Ⅱ표체여병이학특정적관계,이급소우우RNA간예IGF-ⅡmRNA전록,대HepG2세포적영향.방법 이자신배대법수집남통대학부속의원2009년12월-2010년8월간암、암주화원암조직,분석IGF-Ⅱ표체,간병환자혈IGF-Ⅱ농도화병이학특정；이siRNA전염HepG2세포,간예IGF-ⅡmRNA전록,이반전록PCR、정량PCR화ELISA법,분별종전록급단백수평분석；이EdU화Hoechst33342염색분석HepG2세포증식여조망.결과 간암조직IGF-Ⅱ기인(100％,30/30)화단백(83.3％,25/30)고표체,균명현고우암주(46.7％,53.3％)급원암조직(0,0)(균P＜0.01)；간암환자혈IGF-Ⅱ수평(3.74±0.67)ng/L명현고우간경화(3.10±0.63) ng/L、만성간염(1.93 ±0.17) ng/L급건강대조(1.14 ±0.14) ng/L(P＜0.001),여성별、년령、종류대소화AFP농도간미견명현상관,단여HBV감염유관(t=5.390,P＜0.001).체외연구현시IGF-Ⅱ siRNA성공전염HepG2세포후,IGF-Ⅱ표체재전록화단백수평상명현수억,정제량화시간의뢰；억제IGF-Ⅱ mRNA전록,HepG2세포조망화대아매소민감성균명현증가.결론 IGF-Ⅱ표체여HCC진전밀절상관,간예기mRNA전록가촉진암세포조망,증강화료약물민감성.
Objective To explore the expression and pathological features of insulin-like growth factor-Ⅱ (IGF-Ⅱ) in tissues and sera of hepatocellular carcinoma (HCC) patients and the siRNA-mediated inhibition of IGF-Ⅱ mRNA transcription in human HepG2 cells.Methods From December 2009 to August 2010,the self-control HCC,paracancerous and distal cancerous tissues were collected to analyze the expression of IGF-Ⅱ.The serum levels of IGF-Ⅱ expression were detected for pathological features.IGF-Ⅱ expression in HepG2 cells was intervened by siRNA.IGF-Ⅱ mRNA or IGF-Ⅱ level and analyzed by reverse transcription-polymerase chain reaction (RT-PCR),real-time PCR or enzyme-linked immunosorbent assay (ELISA).And the ratio of cell apoptosis was analyzed by EdU/Hoechst33342.Results The levels of IGF-Ⅱ expression in HCC tissues at mRNA (100％,30/30) or protein (83.3％,25/30) were significantly higher (P ＜0.01) than those in para-cancerous (46.7％,53.3％) or distal cancerous tissues (0,0).The serum level of IGF-Ⅱ was significantly higher in HCC patients(3.74 ±0.67) ng/L than that in cases with benign liver diseases (1.93 ± 0.17)ng/L and controls (1.14 ± 0.14)ng/L (P ＜ 0.001).The expression of I GF-Ⅱ in the HCC group was associated with HBV infection (t =5.390,P ＜ 0.001).After siRNA transfection,the expression of IGF-Ⅱ decreased significantly in HepG2 cells at mRNA or protein levels.The down-regulated expression of IGF-Ⅱ was dependent on the dose and time of IGF-Ⅱ siRNA.And the apoptotic index of HepG2 cells and the sensitivity to adriamycin both increased.Conclusion The expression of IGF-Ⅱ is closely associated with the progression of HCC.And the intervening of its transcription may promote apoptosis and sensitize to adriamycin.
