CAJ | 학술논문

目的探讨Ghrelin治疗高血压和胰岛素抵抗的作用及机制.方法雄性SD大鼠32只,计算机随机分为正常对照组(A组,n=16)和果糖诱导组(B组,n=16).B组10％果糖饮水共4周,建立高血压伴胰岛素抵抗大鼠模型.A组分为GA1组(n=8)给予等容积生理盐水,GA2组(n=8)给予50 nmol/kg Ghrelin；B组分为GB1组(n=8)给予等容积生理盐水,GB2组给予50 nmol/kg Ghrelin,各组均分别于每天两次腹膜内注射方式给药共持续6周.测定大鼠尾动脉压、血糖、血脂、血浆胰岛素和15-F21-isoprostane等生化指标以及计算稳态模型胰岛素抵抗指数(HOMA-IR).结果 GB2组大鼠3周后收缩压开始出现明显下降(P＜0.05),第5周血压下降达到稳态,高于GA1组[(127±5)比(120±6)mm Hg,P＜0.05,1 mm Hg =0.133 kPa].GB2组大鼠血浆胰岛素浓度和HOMA-IR均较GB1组明显低[(9.6±2.6)比(13.1 ±3.6) μU/ml,P＜0.05;1.92±0.12比2.78 ±0.14,P＜0.01].GB2组血浆15-F2t-isoprostane含量明显低于GB1组[(75±11)比(102±14) pg/ml,P＜0.01].结论 Ghrelin可能通过抑制氧化应激反应降低果糖诱导大鼠血压和血浆胰岛素浓度,改善机体胰岛素抵抗.
목적 탐토Ghrelin치료고혈압화이도소저항적작용급궤제.방법 웅성SD대서32지,계산궤수궤분위정상대조조(A조,n=16)화과당유도조(B조,n=16).B조10％과당음수공4주,건립고혈압반이도소저항대서모형.A조분위GA1조(n=8)급여등용적생리염수,GA2조(n=8)급여50 nmol/kg Ghrelin；B조분위GB1조(n=8)급여등용적생리염수,GB2조급여50 nmol/kg Ghrelin,각조균분별우매천량차복막내주사방식급약공지속6주.측정대서미동맥압、혈당、혈지、혈장이도소화15-F21-isoprostane등생화지표이급계산은태모형이도소저항지수(HOMA-IR).결과 GB2조대서3주후수축압개시출현명현하강(P＜0.05),제5주혈압하강체도은태,고우GA1조[(127±5)비(120±6)mm Hg,P＜0.05,1 mm Hg =0.133 kPa].GB2조대서혈장이도소농도화HOMA-IR균교GB1조명현저[(9.6±2.6)비(13.1 ±3.6) μU/ml,P＜0.05;1.92±0.12비2.78 ±0.14,P＜0.01].GB2조혈장15-F2t-isoprostane함량명현저우GB1조[(75±11)비(102±14) pg/ml,P＜0.01].결론 Ghrelin가능통과억제양화응격반응강저과당유도대서혈압화혈장이도소농도,개선궤체이도소저항.
Objective To explore the effects and mechanisms of Ghrelin on hypertension and insulin resistance in fructose-fed rats.Methods A total of 32 male Sprague-Dawley (SD) rats were randomized into control (A) and fructose-fed groups (B).Rats in group B were fed with 10％ fructose solution for 4 weeks.Then the rats in group A were randomized into intraperitoneal saline (group GA1) or intraperitoneal 50 nmol/kg Ghrelin (group GA2) and those in group B into intraperitoneal saline (group GB1) or intraperitoneal 50 nmol/kg Ghrelin (group GB2) twice daily for 6 weeks.Caudal arterial pressure was measured weekly.Plasma blood glucose,insulin concentration and lipid profile were measured.And insulin resistance (IR) was calculated by the method of homeostasis model assessment (HOMA).Plasma level of 15-F2t-isoprostane was measured by enzyme-linked immunosorbent assay (ELISA).Results Ghrelin caused a significant reduction of systolic pressure at Week 3 in group GB2 versus group GB1 (P ＜0.05).Maximal effect appeared at Week 5 ((127 ± 5) vs(120 ± 6) mm Hg,P ＜ 0.05,1 mm Hg =0.133kPa),but blood pressure failed to reach normal value.Ghrelin also decreased plasma insulin concentration and HOMA-IR ((9.6 ± 2.6) vs(13.1 ± 3.6) μU/ml,P ＜ 0.05 ; 1.92 ± 0.12 vs 2.78 ± 0.14,P ＜ 0.01).Plasma level of 15-F2t-isoprostane was lower in group GB2 than that in group GB1 ((75 ± 11) vs(102 ± 14) pg/ml,P ＜ 0.01).Conclusion Ghrelin may lower blood pressure,ameliorate insulin resistance and improve insulin sensitivity through inhibiting oxidative stress in fructose-induced rats.