中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
14期
1109-1113
,共5页
念珠菌,白色%生物形态%组氨酸激酶
唸珠菌,白色%生物形態%組氨痠激酶
념주균,백색%생물형태%조안산격매
Candida albicans%Organism forms%Histidine kinase
目的 研究白念珠菌组氨酸激酶基因CHK1基因对白念珠菌某些生物特性的影响.方法 观察CHK1基因突变菌株CHK21、CHK25、CHK26、CHK27的增殖、厚壁孢子形成、芽管形成能力和对刚果红耐受的情况.结果 CHK1基因突变株CHK25、CHK26、CHK27、CHK21的增生能力均低于标准株(6 h:1.36±0.86、1.25 ±0.84、1.05 ±0.79、0.90±0.74比1.54±0.89,P=0.000),CHK21菌株最为明显.CHK1基因突变菌株CHK21、CHK25、CHK26的芽管形成率均低于标准菌株(2 h:5.6％±2.0％、19.5％±6.9％、13.6％±4.8％比29.6％±10.5％,P=0.023、0.028、0.029).在无光线下,标准株和CHK26突变株在14 d形成的厚壁孢子数平均是为3和22个,有光线下分别为60和80个,CHK26形成厚壁孢子的能力比标准株强.无光线下CHK21株无厚壁孢子形成.CHK1基因突变株对刚果红敏感.结论 CHK1基因影响白念珠菌的增殖、厚壁孢子、菌丝的形成及对部分环境压力的耐受.
目的 研究白唸珠菌組氨痠激酶基因CHK1基因對白唸珠菌某些生物特性的影響.方法 觀察CHK1基因突變菌株CHK21、CHK25、CHK26、CHK27的增殖、厚壁孢子形成、芽管形成能力和對剛果紅耐受的情況.結果 CHK1基因突變株CHK25、CHK26、CHK27、CHK21的增生能力均低于標準株(6 h:1.36±0.86、1.25 ±0.84、1.05 ±0.79、0.90±0.74比1.54±0.89,P=0.000),CHK21菌株最為明顯.CHK1基因突變菌株CHK21、CHK25、CHK26的芽管形成率均低于標準菌株(2 h:5.6％±2.0％、19.5％±6.9％、13.6％±4.8％比29.6％±10.5％,P=0.023、0.028、0.029).在無光線下,標準株和CHK26突變株在14 d形成的厚壁孢子數平均是為3和22箇,有光線下分彆為60和80箇,CHK26形成厚壁孢子的能力比標準株彊.無光線下CHK21株無厚壁孢子形成.CHK1基因突變株對剛果紅敏感.結論 CHK1基因影響白唸珠菌的增殖、厚壁孢子、菌絲的形成及對部分環境壓力的耐受.
목적 연구백념주균조안산격매기인CHK1기인대백념주균모사생물특성적영향.방법 관찰CHK1기인돌변균주CHK21、CHK25、CHK26、CHK27적증식、후벽포자형성、아관형성능력화대강과홍내수적정황.결과 CHK1기인돌변주CHK25、CHK26、CHK27、CHK21적증생능력균저우표준주(6 h:1.36±0.86、1.25 ±0.84、1.05 ±0.79、0.90±0.74비1.54±0.89,P=0.000),CHK21균주최위명현.CHK1기인돌변균주CHK21、CHK25、CHK26적아관형성솔균저우표준균주(2 h:5.6％±2.0％、19.5％±6.9％、13.6％±4.8％비29.6％±10.5％,P=0.023、0.028、0.029).재무광선하,표준주화CHK26돌변주재14 d형성적후벽포자수평균시위3화22개,유광선하분별위60화80개,CHK26형성후벽포자적능력비표준주강.무광선하CHK21주무후벽포자형성.CHK1기인돌변주대강과홍민감.결론 CHK1기인영향백념주균적증식、후벽포자、균사적형성급대부분배경압력적내수.
Objective To explore the effects of histidine kinase gene CHK1 on some biological characteristics of Candida albicans.Methods The effects of gene mutation strains of Candida albicans such as CHK21,CHK25,CHK26 and CHK27 were observed on its reproductive ability,formation of chlamydospore and germ tube and tolerance of Congo red.Results The reproductive ability in CHK gene mutation strains CHK25,CHK26,CHK27,CHK21 was weaker than that of wild strains(6 h:1.36 ±0.86,1.25 ± 0.84,1.05 ± 0.79,0.90 ± 0.74 vs 1.54 ± 0.89,P =0.000).And CHK21 was the most obvious.The formation of germ tube in CHK gene mutation strains CHK21,CHK25,CHK26 and CHK27 was weaker than that of wild strains (2 h:5.6％ ± 2.0％,19.5％ ± 6.9％,13.6％ ± 4.8％ vs 29.6％ ± 10.5％,P =0.023,0.028,0.029).Under no light,the mean number of chlamydospore in wild and CHK26 strains was 3 and 22 respectively.With light,the mean number was changed to 60 and 80.So the formation ability of chlamydospore in CHK26 was stronger than other strains.CHK21 could not produce chlamydospore under no light.The mutation strain of CHK1 was sensitive to Congo red.Conclusion CHK1 affect the reproduction and formation of chlamydospore and hypha and the tolerance to some environmental pressures of Candida albicars.
