中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
19期
1499-1502
,共4页
黄志芳%吕双红%朱剑%杨志岗%宋亚昆%杜丽欣%陈显达%胡海旭%吴东颖
黃誌芳%呂雙紅%硃劍%楊誌崗%宋亞昆%杜麗訢%陳顯達%鬍海旭%吳東穎
황지방%려쌍홍%주검%양지강%송아곤%두려흔%진현체%호해욱%오동영
间质干细胞%免疫%关节炎%细胞增殖
間質榦細胞%免疫%關節炎%細胞增殖
간질간세포%면역%관절염%세포증식
Mesenchymal stem cells%Immunity%Arthritis%Cell proliferation
目的 探讨人脐带间充质干细胞(hUCMSC)对脊柱关节炎(SpA)患者外周血单个核细胞(PBMC)体外增殖的抑制作用.方法 采用随机区组设计或配对设计,将12例SpA患者的PBMC与hUCMSC共培养或单独培养,CCK-8法检测PBMC增殖,并以流式细胞术检测其细胞周期分布；同时将hUCMSC的作用与SpA患者临床资料进行相关分析.结果 hUCMSC抑制SpA患者PBMC体外增殖,比例越大抑制作用越强(P＜0.01),直接接触共培养的抑制作用强于Transwell小室培养(57％±17％比32％±12％),两组比较差异有统计学意义(P＜0.01);hUCMSC使处于G1期的PBMC增多(86％±3％比68％±5％),处于(S+G2)期的PBMC减少(8％±3％比26％±5％),两组比较差异有统计学意义(P＜0.01);hUCMSC的抑制作用与SpA患者的临床资料无相关性.结论 hUCMSC能够抑制SpA患者PBMC的体外增殖,在SpA的临床治疗中具有潜在的应用前景.
目的 探討人臍帶間充質榦細胞(hUCMSC)對脊柱關節炎(SpA)患者外週血單箇覈細胞(PBMC)體外增殖的抑製作用.方法 採用隨機區組設計或配對設計,將12例SpA患者的PBMC與hUCMSC共培養或單獨培養,CCK-8法檢測PBMC增殖,併以流式細胞術檢測其細胞週期分佈；同時將hUCMSC的作用與SpA患者臨床資料進行相關分析.結果 hUCMSC抑製SpA患者PBMC體外增殖,比例越大抑製作用越彊(P＜0.01),直接接觸共培養的抑製作用彊于Transwell小室培養(57％±17％比32％±12％),兩組比較差異有統計學意義(P＜0.01);hUCMSC使處于G1期的PBMC增多(86％±3％比68％±5％),處于(S+G2)期的PBMC減少(8％±3％比26％±5％),兩組比較差異有統計學意義(P＜0.01);hUCMSC的抑製作用與SpA患者的臨床資料無相關性.結論 hUCMSC能夠抑製SpA患者PBMC的體外增殖,在SpA的臨床治療中具有潛在的應用前景.
목적 탐토인제대간충질간세포(hUCMSC)대척주관절염(SpA)환자외주혈단개핵세포(PBMC)체외증식적억제작용.방법 채용수궤구조설계혹배대설계,장12례SpA환자적PBMC여hUCMSC공배양혹단독배양,CCK-8법검측PBMC증식,병이류식세포술검측기세포주기분포；동시장hUCMSC적작용여SpA환자림상자료진행상관분석.결과 hUCMSC억제SpA환자PBMC체외증식,비례월대억제작용월강(P＜0.01),직접접촉공배양적억제작용강우Transwell소실배양(57％±17％비32％±12％),량조비교차이유통계학의의(P＜0.01);hUCMSC사처우G1기적PBMC증다(86％±3％비68％±5％),처우(S+G2)기적PBMC감소(8％±3％비26％±5％),량조비교차이유통계학의의(P＜0.01);hUCMSC적억제작용여SpA환자적림상자료무상관성.결론 hUCMSC능구억제SpA환자PBMC적체외증식,재SpA적림상치료중구유잠재적응용전경.
Objective To explore the inhibitory effects of human umbilical cord-derived mesenchymal stem cells (hUCMSC) on the proliferation of peripheral blood mononuclear cells (PBMC) from spondyloarthritis (SpA) patients.Methods A total of 12 SpA patients at Chinese PLA General Hospital were recruited from May 2012 to October 2012.Information on demographic characteristics,disease and functional activity was collected.Isolated PBMC were stimulated by phytohemagglutinin (PHA,1 μg/ml) in the presence or absence of hUCMSC.The proliferation of hUCMSC was suppressed by irradiation with Co60(30 Gy) before co-culturing with PBMC.The proliferation of PBMC was determined by Cell Counting Kit-8(CCK-8).Cell cycle profiles of PBMC were analyzed by flow cytometry.The association of inhibitory effect of hUCMSC with the disease and functional activity of SpA patients was examined.Results After coculturing with hUCMSC by cell-to-cell contact for 5 days,the proliferation of PBMC stimulated by PHA (1 μg/ml)was significantly inhibited by hUCMSC in a dose-dependent manner.The inhibition rate of the proliferation of PBMC cocultured with hUCMSC by cell-to-cell contact was higher than that by Transwell culture (57％ ±17％ vs 32％ ± 12％,P ＜0.01).Compared to PBMC cultured alone,a larger number of PBMC cocultured with hUCMSC were in phase G1 (86％ ± 3％ vs 68％ ± 5％,P ＜ 0.01) while a lower number of cells in phases S and G2(8％ ± 3％ vs 26％ ± 5％,P ＜ 0.01).No association was found between the inhibitory effect of hUCMSC and the disease and functional activity.Conclusion The proliferation of PBMC from SpA patients may be inhibited by hUCMSC.And hUCMSC have therapeutic potentials for SpA patients.
