中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
20期
1559-1562
,共4页
秦彩虹%赵守琴%杨琳%王阳
秦綵虹%趙守琴%楊琳%王暘
진채홍%조수금%양림%왕양
中耳炎,伴渗出液%T淋巴细胞,辅助诱导%白细胞介素4%干扰素Ⅱ型%流式微球分析法
中耳炎,伴滲齣液%T淋巴細胞,輔助誘導%白細胞介素4%榦擾素Ⅱ型%流式微毬分析法
중이염,반삼출액%T림파세포,보조유도%백세포개소4%간우소Ⅱ형%류식미구분석법
Otitis media with effusion%T-lymphocytes,helper-inducer%Interleukin-4%Interferon type Ⅱ%Cytometric bead array
目的 探讨Th2/Th1细胞失衡模式在慢性分泌性中耳炎发病机制中的作用.方法 通过流式细胞技术(FCM)及流式微球分析法(CBA法)检测Th2型细胞因子白细胞介素4(IL-4)和Th1型细胞因子干扰素γ(IFN-γ)在2011年4月至2012年7月就诊于首都医科大学附属北京同仁医院的30例慢性分泌性中耳炎患者外周血及中耳积液及20名健康对照人群外周血中的表达,对比病例组及健康对照组外周血,病例组外周血及中耳积液之间IL-4和IFN-γ水平以及两者比例变化.结果 与健康对照组相比,病例组外周血IL-4水平显著高,差异有统计学意义(P ＜0.05)；IFN-γ阳性细胞/IL-4阳性细胞在病例组明显低于健康对照组,差异有统计学意义(17±13比35±19,P＜0.05).而病例组IFN-γ水平略低于健康对照组,差异无统计学意义(12％±8％比13％±8％,P＞0.05)；病例组外周血与中耳积液中IL-4、IFN-γ水平差异无统计学意义.结论 Th2活化亢进,Th2/Th1平衡失衡,可能是慢性分泌性中耳炎发病的重要基础；过敏反应主要在全身起作用,中耳局部的免疫反应有待进一步进行探索.
目的 探討Th2/Th1細胞失衡模式在慢性分泌性中耳炎髮病機製中的作用.方法 通過流式細胞技術(FCM)及流式微毬分析法(CBA法)檢測Th2型細胞因子白細胞介素4(IL-4)和Th1型細胞因子榦擾素γ(IFN-γ)在2011年4月至2012年7月就診于首都醫科大學附屬北京同仁醫院的30例慢性分泌性中耳炎患者外週血及中耳積液及20名健康對照人群外週血中的錶達,對比病例組及健康對照組外週血,病例組外週血及中耳積液之間IL-4和IFN-γ水平以及兩者比例變化.結果 與健康對照組相比,病例組外週血IL-4水平顯著高,差異有統計學意義(P ＜0.05)；IFN-γ暘性細胞/IL-4暘性細胞在病例組明顯低于健康對照組,差異有統計學意義(17±13比35±19,P＜0.05).而病例組IFN-γ水平略低于健康對照組,差異無統計學意義(12％±8％比13％±8％,P＞0.05)；病例組外週血與中耳積液中IL-4、IFN-γ水平差異無統計學意義.結論 Th2活化亢進,Th2/Th1平衡失衡,可能是慢性分泌性中耳炎髮病的重要基礎；過敏反應主要在全身起作用,中耳跼部的免疫反應有待進一步進行探索.
목적 탐토Th2/Th1세포실형모식재만성분비성중이염발병궤제중적작용.방법 통과류식세포기술(FCM)급류식미구분석법(CBA법)검측Th2형세포인자백세포개소4(IL-4)화Th1형세포인자간우소γ(IFN-γ)재2011년4월지2012년7월취진우수도의과대학부속북경동인의원적30례만성분비성중이염환자외주혈급중이적액급20명건강대조인군외주혈중적표체,대비병례조급건강대조조외주혈,병례조외주혈급중이적액지간IL-4화IFN-γ수평이급량자비례변화.결과 여건강대조조상비,병례조외주혈IL-4수평현저고,차이유통계학의의(P ＜0.05)；IFN-γ양성세포/IL-4양성세포재병례조명현저우건강대조조,차이유통계학의의(17±13비35±19,P＜0.05).이병례조IFN-γ수평략저우건강대조조,차이무통계학의의(12％±8％비13％±8％,P＞0.05)；병례조외주혈여중이적액중IL-4、IFN-γ수평차이무통계학의의.결론 Th2활화항진,Th2/Th1평형실형,가능시만성분비성중이염발병적중요기출；과민반응주요재전신기작용,중이국부적면역반응유대진일보진행탐색.
Objective To explore the shift of Th2/Th1 type cytokine profiles in middle ear and peripheral blood of chronic otitis media with effusion (COME) patients.Methods Middle ear effusions (MEEs) and peripheral bloods were collected from 30 COME patients between April 2011 and July 2012 at Department of Otolaryngology,Beijing Tongren Hospital,Capital Medical University.Peripheral blood samples were collected from 20 healthy controls.The expressions of IFN-γ (Th1 cytokine) and IL-4 (Th2 cytokine) in peripheral blood and middle ear fluid were assessed by flow cytometry and cytometric bead array.The expression levels of cytokines in peripheral blood were compared between patient and control groups.Moreover,the levels of cytokines in the Middle ear fluid and the supernatant of COME patients were also compared.Results Compared to the control group,the level of IL-4 in peripheral blood of COME patients significantly increased(0.7％ ±0.8％ vs 2.4％ ±4.5％,P ＜0.05).The ratio of IFN-γ positive cells/IL-4 positive ceils was significantly lower in peripheral blood of COME patients than that of control group(17 ± 13 vs 35 ± 19,P ＜ 0.05).However,the level of IFN-γ showed no significant difference between two groups(12％ ±8％ vs 13％ ±8％,P ＞0.05).The levels of IL-4 and IFN-γ in middle ear fluid and the supematant of the case group showed no significant difference.Conclusions COME may be a Th2 predominant disease.Th1/Th2 imbalance is probably involved in the pathogenesis of COME.The role of the allergic reaction in middle ear microenvironment remains uncertain.
