CAJ | 학술논문

目的观察生命早期免疫应激对黄体生成素(LH)脉冲分泌的长期影响及其作用机制.方法 SD大鼠在出生后第3、5天腹腔注射内毒素50 μg/kg(实验组)和生理盐水(对照组).在生后12周时行双侧卵巢切除术并植入17β-雌二醇胶囊,同时行动物心导管插管,留置静脉采血装置.对其每5 min采血25μl,共5h.在基础值后2h,两组动物给予静脉注射内毒素25 μg/kg或者生理盐水300μl.用RT-PCR方法测定给药3h后内侧视前区(mPOA)中促肾上腺激素释放激素(CRH)及其受体(CRH-R1和CRH-R2)的基因表达.结果两组成年动物基础水平的LH分泌差异无统计学意义,而静脉注射内毒素后实验组动物的LH分泌抑制要较对照组明显的多(P＜0.05).基础水平的CRH-R1 mRNA在两组中没有区别,而静脉注射LPS后实验组的CRH-R1 mRNA表达较对照组明显增强.而CRH及CRH-R2mRNA则在各组表达均差异无统计学意义.结论生命早期的免疫应激能致敏成年时期同型应激导致的对LH分泌的抑制作用,其机制可能是通过上调mPOA中的CRH-R1的基因表达所致.
목적 관찰생명조기면역응격대황체생성소(LH)맥충분비적장기영향급기작용궤제.방법 SD대서재출생후제3、5천복강주사내독소50 μg/kg(실험조)화생리염수(대조조).재생후12주시행쌍측란소절제술병식입17β-자이순효낭,동시행동물심도관삽관,류치정맥채혈장치.대기매5 min채혈25μl,공5h.재기출치후2h,량조동물급여정맥주사내독소25 μg/kg혹자생리염수300μl.용RT-PCR방법측정급약3h후내측시전구(mPOA)중촉신상선격소석방격소(CRH)급기수체(CRH-R1화CRH-R2)적기인표체.결과 량조성년동물기출수평적LH분비차이무통계학의의,이정맥주사내독소후실험조동물적LH분비억제요교대조조명현적다(P＜0.05).기출수평적CRH-R1 mRNA재량조중몰유구별,이정맥주사LPS후실험조적CRH-R1 mRNA표체교대조조명현증강.이CRH급CRH-R2mRNA칙재각조표체균차이무통계학의의.결론 생명조기적면역응격능치민성년시기동형응격도치적대LH분비적억제작용,기궤제가능시통과상조mPOA중적CRH-R1적기인표체소치.
Objective To explore whether neonatal endotoxin challenge alters reproductive hormone secretion in adult female rats.Methods Rats were intraperitoneally injected with lipopolysaccharide (LPS)or normal saline at postnatal days 3 and 5.At Week 12,the animals underwent bilateral oophorectomy and were exposed to LPS or saline.The secretion levels of luteinizing hormone (LH) and estradiol were measured.Results The LPS injection in adult neonatal saline rats caused the suppressed secretion of LH and estradiol.However,the decreased secretion of both LH and estradiol were much more apparent in adult neonatal LPS-treated rats.Conclusion The neonatal LPS challenge alters the secretions of LH and estradiol during adulthood.And the mechanism may be mediated by epigenetic programming of hypothalamicpituitary-gonadal axis.