CAJ | 학술논문

目的探讨微小RNA(miRNA-222、miRNA-181、miRNA-216)在肝癌患者血清中的异常表达及其临床价值.方法采用实时定量PCR方法检测广东省人民医院2009年6月至2010年9月收治的49例原发性肝癌患者首次确诊时的血清标本及25名正常人血清中上述3个miRNAs的表达水平,并分析它们表达差异及其同肝癌临床病理参数、生存预后的关系.结果 miR-181及miR-216在肝癌患者和正常人血清中表达差异均无统计学意义(均P ＞0.05),而miR-222在肝癌患者血清中表达水平显著上调(6.1±6.6比1.2±0.6,P＜0.01).以miR-222表达量上调的中位倍数(3倍)将49例肝癌患者分成低表达组(25例)和高表达组(24例).miR-222的高表达同肝硬化(P＜0.01)、肿瘤数目(P=0.013)、门静脉癌栓(P=0.002)、TNM分期(P=0.020)相关,而与血清甲胎蛋白水平、肿瘤直径及乙肝表面抗原无关.Kaplan-Meier生存分析显示miR-222的高表达组患者的中位总生存期明显短于低表达组(8.7个月比16.5个月,P=0.036).Cox比例风险模型显示TNM分期和miR-222表达水平是两个主要的独立预后因子.结论血清miR-222在肝癌患者表达上调,可能有助于肝癌患者的预后预测.
목적 탐토미소RNA(miRNA-222、miRNA-181、miRNA-216)재간암환자혈청중적이상표체급기림상개치.방법 채용실시정량PCR방법검측광동성인민의원2009년6월지2010년9월수치적49례원발성간암환자수차학진시적혈청표본급25명정상인혈청중상술3개miRNAs적표체수평,병분석타문표체차이급기동간암림상병리삼수、생존예후적관계.결과 miR-181급miR-216재간암환자화정상인혈청중표체차이균무통계학의의(균P ＞0.05),이miR-222재간암환자혈청중표체수평현저상조(6.1±6.6비1.2±0.6,P＜0.01).이miR-222표체량상조적중위배수(3배)장49례간암환자분성저표체조(25례)화고표체조(24례).miR-222적고표체동간경화(P＜0.01)、종류수목(P=0.013)、문정맥암전(P=0.002)、TNM분기(P=0.020)상관,이여혈청갑태단백수평、종류직경급을간표면항원무관.Kaplan-Meier생존분석현시miR-222적고표체조환자적중위총생존기명현단우저표체조(8.7개월비16.5개월,P=0.036).Cox비례풍험모형현시TNM분기화miR-222표체수평시량개주요적독립예후인자.결론 혈청miR-222재간암환자표체상조,가능유조우간암환자적예후예측.
Objective To investigate the abnormal expression of microRNAs (miR-216,miR-222,miR-181) in the serum of patients with hepatocellular carcinoma (HCC) and its clinical significance.Methods Serum miRNAs expression was investigated in 49 patients with HCC and 25 healthy normal controls by using real-time PCR technique,and then correlations between miRNAs expression and the clinicopathological features and prognosis of HCC patients were evaluated.Results No differences were observed between the HCC patients and healthy controls with respect to the expressions of scrum miR-181and miR-216 (P ＞ 0.05),while miR-222 was found to be significantly overexpressed in HCC serum samples (6.1 ±6.6 vs 1.2 ±0.6,P ＜0.01).According to the median fold change of miR-222 (3-fold) in all HCC serum samples,we divided the 49 patients into two groups:a low expression group (25 patients) and a high expression group (24 patients).High level of miR-222 expression was correlated with cirrhosis (P ＜ 0.01),tumor number (P =0.013),portal vein tumor thrombosis (P =0.002) and TNM stage (P =0.020),while not correlated with serum alpha-fetoprotein level,tumor size and HbsAg.Kaplan-Meier survival analysis showed that the median overall survival in the high miR-222 expression group was significantly shorter than that in the low expression group (8.7 months vs 16.5 months,P =0.036).In multivariate Cox analysis,TNM stage and serum miR-222 expression were two independent prognostic factors.Conclusion Serum miR-222,upregulated in HCC,maybe helpful in prognosis of HCC patients.