中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
27期
2164-2166
,共3页
赵伟%郭梦翔%项道满%周瑾
趙偉%郭夢翔%項道滿%週瑾
조위%곽몽상%항도만%주근
角膜新生血管化%渗漏%周细胞%内皮细胞
角膜新生血管化%滲漏%週細胞%內皮細胞
각막신생혈관화%삼루%주세포%내피세포
Corneal neovascularization%Leakage%Pericytes%Endothelial cells
目的 探讨周细胞对大鼠角膜新生血管渗漏的影响.方法 建立角膜基质层间植入微粒体诱生新生血管的大鼠模型,数字法随机分配大鼠一只眼为实验组,另一只眼为对照组,实验组植入含有血管内皮细胞生长因子(VEGF)和血小板衍生生长因子-B中和抗体(抗PDGF-B)的微粒体,对照组植入含有VEGF和磷酸盐缓冲液(PBS)的微粒体,术后5d伊文氏兰示踪法检测新生血管渗漏率,角膜称重,α平滑肌激动蛋白抗体(抗α-SMA)和血小板内皮细胞黏附分子抗体(抗CD31)对角膜切片及铺片行双重免疫荧光染色法观察并计算周细胞包裹指数(MPI).结果 实验组MPI为11.3％,角膜重量为(8.96±1.09) mg,新生血管渗漏率为(0.68±0.36) μg·ml-·mm-2；对照组MPI为56.5％,角膜重量为(7.36±0.56) mg,新生血管渗漏率为(0.24±0.07) μg· ml-1·mm4,3个指标在实验组和对照组之间比较差异均有统计学意义(PM1＜0.01,P渗漏率=0.01,P重量=0.01).结论 周细胞具有抑制角膜新生血管渗漏的作用,为新生血管性疾病的抗渗漏治疗提供了理论指导.
目的 探討週細胞對大鼠角膜新生血管滲漏的影響.方法 建立角膜基質層間植入微粒體誘生新生血管的大鼠模型,數字法隨機分配大鼠一隻眼為實驗組,另一隻眼為對照組,實驗組植入含有血管內皮細胞生長因子(VEGF)和血小闆衍生生長因子-B中和抗體(抗PDGF-B)的微粒體,對照組植入含有VEGF和燐痠鹽緩遲液(PBS)的微粒體,術後5d伊文氏蘭示蹤法檢測新生血管滲漏率,角膜稱重,α平滑肌激動蛋白抗體(抗α-SMA)和血小闆內皮細胞黏附分子抗體(抗CD31)對角膜切片及鋪片行雙重免疫熒光染色法觀察併計算週細胞包裹指數(MPI).結果 實驗組MPI為11.3％,角膜重量為(8.96±1.09) mg,新生血管滲漏率為(0.68±0.36) μg·ml-·mm-2；對照組MPI為56.5％,角膜重量為(7.36±0.56) mg,新生血管滲漏率為(0.24±0.07) μg· ml-1·mm4,3箇指標在實驗組和對照組之間比較差異均有統計學意義(PM1＜0.01,P滲漏率=0.01,P重量=0.01).結論 週細胞具有抑製角膜新生血管滲漏的作用,為新生血管性疾病的抗滲漏治療提供瞭理論指導.
목적 탐토주세포대대서각막신생혈관삼루적영향.방법 건립각막기질층간식입미립체유생신생혈관적대서모형,수자법수궤분배대서일지안위실험조,령일지안위대조조,실험조식입함유혈관내피세포생장인자(VEGF)화혈소판연생생장인자-B중화항체(항PDGF-B)적미립체,대조조식입함유VEGF화린산염완충액(PBS)적미립체,술후5d이문씨란시종법검측신생혈관삼루솔,각막칭중,α평활기격동단백항체(항α-SMA)화혈소판내피세포점부분자항체(항CD31)대각막절편급포편행쌍중면역형광염색법관찰병계산주세포포과지수(MPI).결과 실험조MPI위11.3％,각막중량위(8.96±1.09) mg,신생혈관삼루솔위(0.68±0.36) μg·ml-·mm-2；대조조MPI위56.5％,각막중량위(7.36±0.56) mg,신생혈관삼루솔위(0.24±0.07) μg· ml-1·mm4,3개지표재실험조화대조조지간비교차이균유통계학의의(PM1＜0.01,P삼루솔=0.01,P중량=0.01).결론 주세포구유억제각막신생혈관삼루적작용,위신생혈관성질병적항삼루치료제공료이론지도.
Objective To explore the effects of pericytes on the leakage of rat corneal neovascularization (CNV).Methods CNV was induced by micropocket assay in rats.Two eyes of the same rat were divided randomly into experimental and control groups.The experimental group received the VEGF + anti-PDGF-B pellet while the control group the VEGF + PBS pellet.Corneal samples were excised at Day 5 postoperation.CNV leakage was measured by Evans blue method.Pericyte coverage index (MPI) was applied to quantify the pericyte coverage through double immunofluorescent stain of frozen sections of corneas with CD31 as endothelial and α-smooth muscle actin (α-SMA) as pericyte marker.Corneal weight was measured.Results In the control group,MPI was 56.5％,cornea weight (7.36 ± 0.56) mg and CNV permeability rate (0.24 ± 0.07) μg · ml-1 · mm-2 In the experimental group,MPI was 11.3％,cornea weight (8.96 ± 1.09) mg and CNV permeability rate (0.68 ±0.36) μg · ml-1 ·mm-2.The intergroup difference was statistically significant (PMPI ＜ 0.01,Ppermeaility rate =0.01,Pweight =0.01).Conclusion Pericytes inhibit the leakage of rat CNV.Such findings may guide the clinical management of hyperpermeability.
