CAJ | 학술논문

目的研究细胞增殖上调因子(URGCP)在预测肝癌的预后和在肝癌的进展和发展中的潜在价值.方法取材中山大学肿瘤医院病理科278例肝癌石蜡包埋标本,标本患者的手术日期在1995-2003年,在15个肝癌细胞株及10对肝癌与癌旁组织进行实时定量PCR和免疫印迹检测URGCP的表达.采用免疫组织化学检测278例肝癌样本,对URGCP表达进行分析,统计与临床数据的相关性,分析URGCP蛋白与肝癌患者预后的相关性.结果免疫组化显示,URGCP在所有测试的肝癌细胞株及122例(43.8％)石蜡包埋肝癌标本中高表达.URGCP主要为胞质表达,随着肝癌级别的增高,URGCP的表达水平增高.URGCP的表达水平与临床分期显著正相关,与肝癌患者的预后生存显著负相关,与患者年龄、肿瘤个数、大小、AFP不相关.结论 URGCP在促进肝癌细胞增殖和肿瘤形成中起着重要的作用,并可能代表了一种新的预后标志物和治疗疾病的靶标.
목적 연구세포증식상조인자(URGCP)재예측간암적예후화재간암적진전화발전중적잠재개치.방법 취재중산대학종류의원병이과278례간암석사포매표본,표본환자적수술일기재1995-2003년,재15개간암세포주급10대간암여암방조직진행실시정량PCR화면역인적검측URGCP적표체.채용면역조직화학검측278례간암양본,대URGCP표체진행분석,통계여림상수거적상관성,분석URGCP단백여간암환자예후적상관성.결과 면역조화현시,URGCP재소유측시적간암세포주급122례(43.8％)석사포매간암표본중고표체.URGCP주요위포질표체,수착간암급별적증고,URGCP적표체수평증고.URGCP적표체수평여림상분기현저정상관,여간암환자적예후생존현저부상관,여환자년령、종류개수、대소、AFP불상관.결론 URGCP재촉진간암세포증식화종류형성중기착중요적작용,병가능대표료일충신적예후표지물화치료질병적파표.
Objective To explore the potential value of up-regulator of cell proliferation (URGCP) as a biomarker for predicting the prognosis of hepatocellular carcinoma (HCC).Methods The expression of URGCP was analyzed in 15 HCC cell lines and in 10 pairs of HCC and adjacent tissues with reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.The expression of URGCP in 278 paraffin-embedded,archived clinical HCC samples was analyzed by immunohistochemistry (IHC) and statistic analysis conducted to examine the relationship of prognosis and URGCP expression.Results IHC analysis revealed a high expression of URGCP in all HCC cell lines and in 122/278 (43.8％) paraffinembedded archived HCC specimens.The expression level of URGCP was significantly correlated with clinical staging and poor patient survival of HCC in the study cohort and in various clinical subgroups,but not correlated with HCC patient age,tumor size,tumor number or alpha-fetoprotein level.Conclusion URGCP plays an important role in promoting the proliferation and tumorigenesis of HCC and may represent a novel prognostic biomarker and therapeutic target for the disease.