CAJ | 학술논문

目的评价五色流式细胞术分类计数外周血白细胞的临床应用价值.方法选取2010年9至12月北京大学第一医院就诊的265例患者静脉全血标本,以显微镜检查为参考方法,同时用LH750全自动血细胞分析仪和FC500流式细胞仪分类计数白细胞,统计分析显微镜、血细胞分析仪和流式细胞术分类计数外周血白细胞的相关性.选取2010年8至11月北京大学第一医院、北京市道培医院和北京军区总医院的40例患者的静脉全血标本,以显微镜检查为参考方法,同时用LH750全自动血细胞分析仪和NAVIOS流式细胞仪分类计数白细胞,统计分析显微镜、血细胞分析仪和流式细胞术分类计数外周血白细胞的相关性和流式细胞术的临床诊断效率.结果 FC500流式细胞仪采用仪器自动分析软件进行分类,与显微镜分类计数5种白细胞均具有一定相关性(均P＜0.01),其中以淋巴细胞、中性粒细胞和嗜酸粒细胞的相关性最佳(r=0.955、0.928、0.876).NAVIOS 流式细胞仪采用手工输入设门策略进行分类,与显微镜方法比较除嗜碱粒细胞外均具有良好的相关性(r ＞0.700,均P＜0.01),其中对检出中性粒细胞、淋巴细胞和原始细胞的相关性较高(r=0.950、0.915、0.852).以显微镜法分类计数外周血不成熟粒细胞1％为临界值,流式细胞术检出不成熟粒细胞的敏感度为87％,特异度为76％.以显微镜法检查外周血原始细胞0.5％为临界值,流式细胞术检出的敏感度为100％,特异度为92％.结论应用五色流式细胞术在不同流式细胞仪上分类计数外周血白细胞与显微镜法均具有良好的相关性,对检出原始细胞和不成熟粒细胞具有较高的敏感度.
목적 평개오색류식세포술분류계수외주혈백세포적림상응용개치.방법 선취2010년9지12월북경대학제일의원취진적265례환자정맥전혈표본,이현미경검사위삼고방법,동시용LH750전자동혈세포분석의화FC500류식세포의분류계수백세포,통계분석현미경、혈세포분석의화류식세포술분류계수외주혈백세포적상관성.선취2010년8지11월북경대학제일의원、북경시도배의원화북경군구총의원적40례환자적정맥전혈표본,이현미경검사위삼고방법,동시용LH750전자동혈세포분석의화NAVIOS류식세포의분류계수백세포,통계분석현미경、혈세포분석의화류식세포술분류계수외주혈백세포적상관성화류식세포술적림상진단효솔.결과 FC500류식세포의채용의기자동분석연건진행분류,여현미경분류계수5충백세포균구유일정상관성(균P＜0.01),기중이림파세포、중성립세포화기산립세포적상관성최가(r=0.955、0.928、0.876).NAVIOS 류식세포의채용수공수입설문책략진행분류,여현미경방법비교제기감립세포외균구유량호적상관성(r ＞0.700,균P＜0.01),기중대검출중성립세포、림파세포화원시세포적상관성교고(r=0.950、0.915、0.852).이현미경법분류계수외주혈불성숙립세포1％위림계치,류식세포술검출불성숙립세포적민감도위87％,특이도위76％.이현미경법검사외주혈원시세포0.5％위림계치,류식세포술검출적민감도위100％,특이도위92％.결론 응용오색류식세포술재불동류식세포의상분류계수외주혈백세포여현미경법균구유량호적상관성,대검출원시세포화불성숙립세포구유교고적민감도.
Objective To explore the clinical application values of five-color flow cytometry for leukocyte differential count in peripheral blood.Methods Leukocyte differentiation in 265 peripheral blood samples collected at Peking University First Hospital from September 2010 to December 2010 was detected by standard microscopic cytology as a reference method.Meanwhile,Beckman-Coulter LH750 hematology analyzer and FC500 flow cytometer were performed.Then the correlations were analyzed between microscopic cytology,hematology analyzer and flow cytometry.Forty blood samples collected at Peking University First Hospital,Beijing Daopei Hospital and General Hospital of Beijing Military Command from August 2010 to November 2010 were analyzed by standard microscopic cytology,Beckman-Coulter LH750 hematology analyzer and NAVIOS flow cytometer.Then the correlations between microscopy,hematology analyzer and flow cytometry were explored to analyze the clinical diagnostic efficiency of flow cytometry.Results Correlation of leukocyte differential count between FC500 flow cytometer and standard microscopic cytology was significant(all P ＜ 0.01).And it was superior in the detection of lymphocytes,neutrophils and eosinaphils (r =0.955,0.928,0.876).Moreover,the correlation of leukocyte differential count between NAVIOS flow cytometer via manual gate and standard microscopic cytology was significant (r ＞ 0.700,all P ＜ 0.01) except for basophils.And it was superior in the detection of neutrophils,lymphocytes and blasts (r =0.950,0.915,0.852).When 1％ was set as the cut-off value of immature granulocytes on standard microscopic cytology,the sensitivity and specificity of flow cytometry was 87％ and 76％ respectively.When 0.5％ was set as the cut-off value of blasts on standard microscopic cytology,the sensitivity and specificity of flow cytometry stood at 100％ and 92％ respectively.Conclusion Five-color flow cytometry is wellcorrelated with standard microscopic cytology for leukocyte differential count in peripheral blood with different flow cytometers,and the sensitivity of detecting blasts and immature granulocytes is very excellent.