CAJ | 학술논문

目的应用抗体芯片分析不同炎症等级釉质上皮型颅咽管瘤组织中炎症细胞因子表达.方法根据邻近肿瘤正常组织交界处的炎症细胞数量对颅咽管瘤进行炎症等级评估,采用抗体芯片对不同炎症等级的颅咽管瘤炎症细胞因子进行检测,运用Western印迹实验对筛查结果进行验证.结果重度炎症组肿瘤组织多种炎性细胞因子,包括促炎因子、趋化因子、某些细胞因子受体等的表达水平较轻度炎症组显著升高.结论不同炎症等级的颅咽管瘤组织中炎症细胞因子的表达存在差异.
목적 응용항체심편분석불동염증등급유질상피형로인관류조직중염증세포인자표체.방법 근거린근종류정상조직교계처적염증세포수량대로인관류진행염증등급평고,채용항체심편대불동염증등급적로인관류염증세포인자진행검측,운용Western인적실험대사사결과진행험증.결과 중도염증조종류조직다충염성세포인자,포괄촉염인자、추화인자、모사세포인자수체등적표체수평교경도염증조현저승고.결론 불동염증등급적로인관류조직중염증세포인자적표체존재차이.
Objective To explore the expression pattern of inflammatory cytokines at various inflammatory levels of adamantinomatous craniopharyngioma by cytokine antibody array.Methods The inflammatory levels of adamantinomatous craniopharyngioma were evaluated on the basis of the number of inflammatory cells at the interface of tumor and normal tissues.And the expression of inflammatory cytokines was examined at various inflammatory levels of adamantinomatous craniopharyngioma by inflammatory cytokine antibody array and the results were verified by Western blot.Results Compared with the mild inflammatory group,the levels of inflammatory cytokines of severe inflammatory group markedly increased,including pro-inflammatory cytokines,chemokines and cytokine receptors.Conclusion Antibody array demonstrates a significant change in cytokine profiles in adamantinomatous craniopharyngioma with severe inflammation,as compared with those with mild inflammation.