中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
32期
2549-2552
,共4页
李茵%温颖%周进学%张振庭
李茵%溫穎%週進學%張振庭
리인%온영%주진학%장진정
肿瘤,鳞状细胞%早期生长反应蛋白质3%细胞增殖%细胞凋亡
腫瘤,鱗狀細胞%早期生長反應蛋白質3%細胞增殖%細胞凋亡
종류,린상세포%조기생장반응단백질3%세포증식%세포조망
Neoplasms,squamous cell%Early growth response protein 3%Cell proliferation%Apoptosis
目的 探讨早期生长反应因子3(EGR3)在口腔鳞状细胞癌(简称鳞癌)组织及肿瘤细胞系中的表达及其对肿瘤细胞生物学行为的影响.方法 收集2008年10月-2009年12月北京口腔医院口腔修复科及河南省肿瘤医院肿瘤外科收治的口腔鳞癌患者肿瘤组织样本、配对癌旁样本共20例,对照组口腔黏膜样本7例.明确EGR3在20例口腔鳞癌肿瘤组织、癌旁组织及人口腔鳞癌细胞系KB和Tca-8113的表达情况.构建EGR3全长基因的表达质粒,转染并观察其对口腔鳞癌细胞系Tca-8113细胞增殖、细胞凋亡作用的影响.结果 EGR3 mRNA在口腔鳞状上皮细胞及癌旁组织相对表达量较高,分别为2.108±0.996和1.721±1.196,在肿瘤组织及口腔鳞癌细胞系中表达量较低,分别为0.007±0.005和0.007 ±0.001(均P＜0.05).转染48 h后,EGR3转染组肿瘤细胞Tca-8113增殖指数显著低于空质粒对照组(0.35 ±0.11比0.82 ±0.21,P＜0.05),Tea-8113中晚期凋亡率(膜联蛋白V/碘化丙啶双阳性细胞群)与空质粒对照组相比差异具有统计学意义(23.12％±6.65％比5.96％±0.98％,P＜0.05).结论 EGR3在口腔鳞癌呈现低表达或表达缺失,EGR3的过表达可显著抑制口腔鳞癌生长、促进肿瘤细胞的凋亡.
目的 探討早期生長反應因子3(EGR3)在口腔鱗狀細胞癌(簡稱鱗癌)組織及腫瘤細胞繫中的錶達及其對腫瘤細胞生物學行為的影響.方法 收集2008年10月-2009年12月北京口腔醫院口腔脩複科及河南省腫瘤醫院腫瘤外科收治的口腔鱗癌患者腫瘤組織樣本、配對癌徬樣本共20例,對照組口腔黏膜樣本7例.明確EGR3在20例口腔鱗癌腫瘤組織、癌徬組織及人口腔鱗癌細胞繫KB和Tca-8113的錶達情況.構建EGR3全長基因的錶達質粒,轉染併觀察其對口腔鱗癌細胞繫Tca-8113細胞增殖、細胞凋亡作用的影響.結果 EGR3 mRNA在口腔鱗狀上皮細胞及癌徬組織相對錶達量較高,分彆為2.108±0.996和1.721±1.196,在腫瘤組織及口腔鱗癌細胞繫中錶達量較低,分彆為0.007±0.005和0.007 ±0.001(均P＜0.05).轉染48 h後,EGR3轉染組腫瘤細胞Tca-8113增殖指數顯著低于空質粒對照組(0.35 ±0.11比0.82 ±0.21,P＜0.05),Tea-8113中晚期凋亡率(膜聯蛋白V/碘化丙啶雙暘性細胞群)與空質粒對照組相比差異具有統計學意義(23.12％±6.65％比5.96％±0.98％,P＜0.05).結論 EGR3在口腔鱗癌呈現低錶達或錶達缺失,EGR3的過錶達可顯著抑製口腔鱗癌生長、促進腫瘤細胞的凋亡.
목적 탐토조기생장반응인자3(EGR3)재구강린상세포암(간칭린암)조직급종류세포계중적표체급기대종류세포생물학행위적영향.방법 수집2008년10월-2009년12월북경구강의원구강수복과급하남성종류의원종류외과수치적구강린암환자종류조직양본、배대암방양본공20례,대조조구강점막양본7례.명학EGR3재20례구강린암종류조직、암방조직급인구강린암세포계KB화Tca-8113적표체정황.구건EGR3전장기인적표체질립,전염병관찰기대구강린암세포계Tca-8113세포증식、세포조망작용적영향.결과 EGR3 mRNA재구강린상상피세포급암방조직상대표체량교고,분별위2.108±0.996화1.721±1.196,재종류조직급구강린암세포계중표체량교저,분별위0.007±0.005화0.007 ±0.001(균P＜0.05).전염48 h후,EGR3전염조종류세포Tca-8113증식지수현저저우공질립대조조(0.35 ±0.11비0.82 ±0.21,P＜0.05),Tea-8113중만기조망솔(막련단백V/전화병정쌍양성세포군)여공질립대조조상비차이구유통계학의의(23.12％±6.65％비5.96％±0.98％,P＜0.05).결론 EGR3재구강린암정현저표체혹표체결실,EGR3적과표체가현저억제구강린암생장、촉진종류세포적조망.
Objective To evaluate the expression of early growth response gene-3 (EGR3) in human oral squamous carcinoma tissue,non-adjacent tissue and oral carcinoma cell lines and detect the effects of EGR3 expression on the biological behaviors of oral squamous carcinoma cell lines.Methods From October 2008 to December 2009,the expression of EGR3 was detected in 20 human oral squamous carcinoma tissue,non-tumor adjacent tissue and 2 oral carcinoma cell lines.The total-length plasmid of EGR3 was constructed and transfected into oral squamous carcinoma line Tca-8113 to observe the proliferation and apoptosis.Results There was a high-level expression of EGR3 mRNA in normal oral squamous epithelial and non-tumor adjacent tissues (2.108 ± 0.996 and 1.721 ± 1.196).And a low-level expression or a loss of EGR3 mRNA in human oral squamous carcinoma tissue and oral carcinoma cell lines (0.007 ±0.005 and 0.007 ±0.001) (both P ＜0.05).Tca-8113 transfected by EGR3 plasmid had a highlevel expression of EGR3 mRNA capable of inhibiting the proliferation of Tca-8113 and promoting its apoptosis dramatically.The stimulating index in overexpression of EGR3 was lower than control group (0.35 ±0.11 vs 0.82 ±0.21,P ＜0.05).After overexpression of EGR3 in Tca-8113,the frequency of middle-Late stage of apoptosis (Annexin-V/PI double positive)was higher than the frequency of vector group (23.12％ ±6.65％ vs 5.96％ ± 0.98％,P ＜ 0.05).Conclusion A low-level expression or a loss of EGR3 in human oral squamous carcinoma and an over-expression of EGR3 in human oral squamous carcinoma may inhibit the proliferation and promote apoptosis of oral squamous carcinoma.
