中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
32期
2584-2586
,共3页
王建军%洪强%胡丛岗%方跃君%汪勇
王建軍%洪彊%鬍叢崗%方躍君%汪勇
왕건군%홍강%호총강%방약군%왕용
食管肿瘤%免疫组织化学%印迹法,蛋白质%结肠癌转移相关基因
食管腫瘤%免疫組織化學%印跡法,蛋白質%結腸癌轉移相關基因
식관종류%면역조직화학%인적법,단백질%결장암전이상관기인
Esophageal neoplasms%Immunohistochemistry%Blotting,Western%Metastasis-associated in colon cancer-1
目的 探讨结肠癌转移相关基因1(MACCl)蛋白在食管癌组织中的表达及其意义.方法 采用免疫组织化学和Western印迹方法检测了2010至2012年金华市广福医院胸外科手术切除的60例[男38例、女22例,年龄(50±12)岁]食管癌组织及相应癌旁组织中MACC1蛋白的表达情况,并分析其与食管癌临床病理因素之间的关系.结果 MACC1蛋白的表达主要位于组织细胞的胞质及胞膜,在食管癌组织中总的阳性表达率明显高于相应癌旁组织[68.3％ (41/60)比25.0％(15/60),P＜0.01];Western印迹结果显示MACC1蛋白在食管癌组织中的表达水平高于癌旁肝组织(0.64±0.05比0.21 ±0.10,P＜0.05).MACC1蛋白的表达水平与食管癌的TNM分期和淋巴结转移及分化程度显著相关(均P＜0.05).结论 MACC1的异常表达与食管癌的发生、发展密切相关.
目的 探討結腸癌轉移相關基因1(MACCl)蛋白在食管癌組織中的錶達及其意義.方法 採用免疫組織化學和Western印跡方法檢測瞭2010至2012年金華市廣福醫院胸外科手術切除的60例[男38例、女22例,年齡(50±12)歲]食管癌組織及相應癌徬組織中MACC1蛋白的錶達情況,併分析其與食管癌臨床病理因素之間的關繫.結果 MACC1蛋白的錶達主要位于組織細胞的胞質及胞膜,在食管癌組織中總的暘性錶達率明顯高于相應癌徬組織[68.3％ (41/60)比25.0％(15/60),P＜0.01];Western印跡結果顯示MACC1蛋白在食管癌組織中的錶達水平高于癌徬肝組織(0.64±0.05比0.21 ±0.10,P＜0.05).MACC1蛋白的錶達水平與食管癌的TNM分期和淋巴結轉移及分化程度顯著相關(均P＜0.05).結論 MACC1的異常錶達與食管癌的髮生、髮展密切相關.
목적 탐토결장암전이상관기인1(MACCl)단백재식관암조직중적표체급기의의.방법 채용면역조직화학화Western인적방법검측료2010지2012년금화시엄복의원흉외과수술절제적60례[남38례、녀22례,년령(50±12)세]식관암조직급상응암방조직중MACC1단백적표체정황,병분석기여식관암림상병리인소지간적관계.결과 MACC1단백적표체주요위우조직세포적포질급포막,재식관암조직중총적양성표체솔명현고우상응암방조직[68.3％ (41/60)비25.0％(15/60),P＜0.01];Western인적결과현시MACC1단백재식관암조직중적표체수평고우암방간조직(0.64±0.05비0.21 ±0.10,P＜0.05).MACC1단백적표체수평여식관암적TNM분기화림파결전이급분화정도현저상관(균P＜0.05).결론 MACC1적이상표체여식관암적발생、발전밀절상관.
Objective To explore the expression of metastasis-associated colon cancer 1 (MACC1) proteins in esophageal carcinoma and neighboring tissues.Methods The expressions of MACC1 were detected in 60 specimens of esophageal carcinoma and neighboring tissues with immunohistochemistry and Western blotting.All the specimens were selected from 2010-2012 of Guangfu Hospital of Jinhua,38 males and 22 females,aged (50 ± 12) years.And the correlations of the expressions of MACC1 proteins with the clinicopathologic features of esophageal carcinoma were also analyzed.Results Expression of MACC1 protein was predominantly located in cytoplasm and membrane.The positivity rates of MACC1 protein were 68.3％ (41/60) in esophageal carcinoma tissue and there were significant differences from those in neighboring tissue (25.0 (15/60),P ＜ 0.01).Western blotting analysis showed that the expression level of MACC1 protein in esophageal carcinoma was greater than that in corresponding adjacent tissues (0.64 ± 0.05 vs 0.21 ±0.10,P ＜0.05).Moreover,the positivity rates and relative expressions of MACC1 showed significant correlations with TNM stage and pathology grade (all P ＜ 0.05).Conclusion The abnormal expression of MACC1 may be associated with malignant progression of esophageal carcinoma.
