CAJ | 학술논문

目的了解临床分离的副溶血弧菌的耐药性及毒力特征.方法通过医生工作站获取患者临床资料并通过实验室信息管理系统(LIS)统计2009至2012年北京同仁医院肠道门诊细菌性腹泻病原菌种类及数量；纸片扩散法检测副溶血弧菌对15种抗生素的耐药表型；PCR技术检测直接溶血毒素(TDH)基因(tdh)、TDH-相关溶血毒素基因(trh)和两个Ⅲ型分泌系统2基因(T3SS2α、T3SS2β).结果副溶血弧菌在细菌性腹泻中所占比例为37.90％(274/723),居于首位.216株副溶血弧菌对氨苄西林、头孢噻吩、头孢呋辛、阿米卡星4种抗生素的耐药率分别为100％ (216/216)、44.91％ (97/216)、24.07％(52/216)、9.26％(20/216).对另外11种抗生素敏感或中度敏感.216株副溶血弧菌中tdh携带率为96.76％(209/216),trh携带率为1.39％ (3/216)；T3SS2携带率为100％,其中213株只携带T3SS2α,2株只携带T3SS2β,1株同时携带T3SS2α和T3SS2β.结论副溶血弧菌对氨苄西林耐药率最高,对其他抗生素不同程度耐药.造成人类腹泻的副溶血弧菌主要是tdh阳性菌株；Ⅲ型分泌系统在菌株致病中起重要作用.
목적 료해림상분리적부용혈호균적내약성급독력특정.방법 통과의생공작참획취환자림상자료병통과실험실신식관리계통(LIS)통계2009지2012년북경동인의원장도문진세균성복사병원균충류급수량；지편확산법검측부용혈호균대15충항생소적내약표형；PCR기술검측직접용혈독소(TDH)기인(tdh)、TDH-상관용혈독소기인(trh)화량개Ⅲ형분비계통2기인(T3SS2α、T3SS2β).결과 부용혈호균재세균성복사중소점비례위37.90％(274/723),거우수위.216주부용혈호균대안변서림、두포새분、두포부신、아미잡성4충항생소적내약솔분별위100％ (216/216)、44.91％ (97/216)、24.07％(52/216)、9.26％(20/216).대령외11충항생소민감혹중도민감.216주부용혈호균중tdh휴대솔위96.76％(209/216),trh휴대솔위1.39％ (3/216)；T3SS2휴대솔위100％,기중213주지휴대T3SS2α,2주지휴대T3SS2β,1주동시휴대T3SS2α화T3SS2β.결론 부용혈호균대안변서림내약솔최고,대기타항생소불동정도내약.조성인류복사적부용혈호균주요시tdh양성균주；Ⅲ형분비계통재균주치병중기중요작용.
Objective To explore drug resistance and virulence characteristics of clinically isolated Vibrio parahaemolyticus (VP).Methods The patient records were reviewed.The laboratory information system was employed to count the quantity of bacterial diarrhea pathogens at our intestinal clinic from 2009 to 2012.The drug resistant phenotype was tested by the method of disk diffusion.Polymerase chain reaction (PCR) was used to detect thermo-stable direct hemolysin (TDH) gene (tdh),TDH-related hemolysin gene (trh) and type Ⅲ secretion system 2 (T3SS2α,T3SS2β).Results The VP infection rate was 37.90％ (274/723).Susceptibility test showed that the resistance rates of ampicillin,cefalotin,cefuroxime and amikacin were 100％ (216/216),44.91％ (97/216),24.07％ (52/216),9.26％ (20/216).The other 11 kinds of antibiotics were sensitive or intermediate.96.76％ (209/216) VP harbored tdh gene,1.39％ (3/216) trh gene and 100％ T3SS2 gene,with 213 strains T3SS2α positive,2 strains T3SS2β positive and 1 strain both T3SS2oα and T3SS2β positive.Conclusion The resistant rate of VP to ampicillin is the highest and VP has different levels of resistance against other antibiotics.Most clinical isolates of VP harbor tdh gene and T3SS2 plays an important role in the pathogenicity of VP.