中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
37期
2957-2960
,共4页
丁爱萍%张瑶%魏恒%罗清双%张淑兰
丁愛萍%張瑤%魏恆%囉清雙%張淑蘭
정애평%장요%위항%라청쌍%장숙란
宫颈肿瘤%T淋巴细胞,调节性%人乳头状瘤病毒16%病毒整合
宮頸腫瘤%T淋巴細胞,調節性%人乳頭狀瘤病毒16%病毒整閤
궁경종류%T림파세포,조절성%인유두상류병독16%병독정합
Uterine cervical neoplasms%T-lymphocytes,regulator%Human papillomavirus 16%Virus integration
目的 探讨人乳头状瘤病毒(HPV) 16 DNA存在状态与调节性T细胞(Treg)表面标志物Foxp3+的表达及外周血Treg/Th17相关细胞因子的关系和在宫颈癌进展中的作用和意义.方法 收集2012年1至10月于中国医科大学附属盛京医院妇科142例HPV16+病例,分为宫颈鳞癌(CC,60例),宫颈上皮内瘤变(CIN,65例),对照组(健康妇女,17例).采用多重实时PCR技术检测宫颈脱落细胞的E2和E6基因,通过E2/E6比值法评估HPV16型DNA的体内存在状态.采用免疫组化方法检测宫颈组织中Foxp3+调节性T细胞表达.采用酶联免疫吸附试验(ELISA)方法检测Treg/Th17相关细胞因子:转化生长因子-β(TGF-β)、白细胞介素(IL)-10、17、21.结果 (1)在HPV16DNA体内同一种存在状态下,CIN和CC组Foxp3+比例及TGF-β、IL-10浓度较对照组明显升高(P＜0.01);IL-17、IL-21浓度较对照组明显降低(P＜0.05);(2)在同一病变条件下,HPV16 DNA整合率随着Foxp3+、TGF-β、IL-10表达增加而升高,随着IL-21、IL-17表达降低而升高;(3)不同HPV16型DNA存在状态的宫颈癌中Foxp3+表达与FIGO分期、组织学分级及淋巴结转移密切相关(P＜0.05),而与年龄无关(P＞0.05).结论 Treg细胞因子与HPV16整合率和宫颈病变严重程度呈正相关,而Th17细胞因子与其呈负相关.Treg/Th17细胞因子在HPV持续感染所致宫颈癌的发生发展中可能起着重要作用.
目的 探討人乳頭狀瘤病毒(HPV) 16 DNA存在狀態與調節性T細胞(Treg)錶麵標誌物Foxp3+的錶達及外週血Treg/Th17相關細胞因子的關繫和在宮頸癌進展中的作用和意義.方法 收集2012年1至10月于中國醫科大學附屬盛京醫院婦科142例HPV16+病例,分為宮頸鱗癌(CC,60例),宮頸上皮內瘤變(CIN,65例),對照組(健康婦女,17例).採用多重實時PCR技術檢測宮頸脫落細胞的E2和E6基因,通過E2/E6比值法評估HPV16型DNA的體內存在狀態.採用免疫組化方法檢測宮頸組織中Foxp3+調節性T細胞錶達.採用酶聯免疫吸附試驗(ELISA)方法檢測Treg/Th17相關細胞因子:轉化生長因子-β(TGF-β)、白細胞介素(IL)-10、17、21.結果 (1)在HPV16DNA體內同一種存在狀態下,CIN和CC組Foxp3+比例及TGF-β、IL-10濃度較對照組明顯升高(P＜0.01);IL-17、IL-21濃度較對照組明顯降低(P＜0.05);(2)在同一病變條件下,HPV16 DNA整閤率隨著Foxp3+、TGF-β、IL-10錶達增加而升高,隨著IL-21、IL-17錶達降低而升高;(3)不同HPV16型DNA存在狀態的宮頸癌中Foxp3+錶達與FIGO分期、組織學分級及淋巴結轉移密切相關(P＜0.05),而與年齡無關(P＞0.05).結論 Treg細胞因子與HPV16整閤率和宮頸病變嚴重程度呈正相關,而Th17細胞因子與其呈負相關.Treg/Th17細胞因子在HPV持續感染所緻宮頸癌的髮生髮展中可能起著重要作用.
목적 탐토인유두상류병독(HPV) 16 DNA존재상태여조절성T세포(Treg)표면표지물Foxp3+적표체급외주혈Treg/Th17상관세포인자적관계화재궁경암진전중적작용화의의.방법 수집2012년1지10월우중국의과대학부속성경의원부과142례HPV16+병례,분위궁경린암(CC,60례),궁경상피내류변(CIN,65례),대조조(건강부녀,17례).채용다중실시PCR기술검측궁경탈락세포적E2화E6기인,통과E2/E6비치법평고HPV16형DNA적체내존재상태.채용면역조화방법검측궁경조직중Foxp3+조절성T세포표체.채용매련면역흡부시험(ELISA)방법검측Treg/Th17상관세포인자:전화생장인자-β(TGF-β)、백세포개소(IL)-10、17、21.결과 (1)재HPV16DNA체내동일충존재상태하,CIN화CC조Foxp3+비례급TGF-β、IL-10농도교대조조명현승고(P＜0.01);IL-17、IL-21농도교대조조명현강저(P＜0.05);(2)재동일병변조건하,HPV16 DNA정합솔수착Foxp3+、TGF-β、IL-10표체증가이승고,수착IL-21、IL-17표체강저이승고;(3)불동HPV16형DNA존재상태적궁경암중Foxp3+표체여FIGO분기、조직학분급급림파결전이밀절상관(P＜0.05),이여년령무관(P＞0.05).결론 Treg세포인자여HPV16정합솔화궁경병변엄중정도정정상관,이Th17세포인자여기정부상관.Treg/Th17세포인자재HPV지속감염소치궁경암적발생발전중가능기착중요작용.
Objective To explore the relationship between the presence of HPV-16 DNA and the expression Treg surface marker Foxp3 +,peripheral blood levels of Th17/Treg cell-associated cytokines and explore their roles and significance in cervical cancer progression.Methods Between January 2012 and October 2012 at Shengjing Hospital of China Medical University,a total of 142 HPV16 positive patients were divided into cervical cancer (CC,n =60),cervical intraepithelial neoplasia (CIN,n =65) and control group (n =17).Cervical liquid-based cytological (LBC) samples were collected to detect E2 and E6 genes of HPV type 16 using multiple real-time polymerase chain reaction (PCR).E2/E6 ratio was used to evaluate the physical status of HPV-16 DNA in host cell genome.The SP immunohistochemical method was used to detect the expressions of FOXP3 in cervical lesions.The concentrations of Th17/Treg cell-associated cytokines were detected by enzyme-linked immunosorbent assay (ELISA).Results Under the same status of HPV16 DNA in vivo,the levels of Foxp3 +,transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) were significantly higher than those of the control group(P ＜ 0.01) while the levels of interleukin-17 (IL-17) and interleukin-21 (IL-21) were significantly lower than those of the control group (P ＜ 0.05).In the same disease,HPV16 DNA integration rate grew with the increases of Foxp3 +,TGF-β and IL-10 while IL-17 and IL-21 were opposite.In the different status of HPV16 type DNA,the expression of Foxp3 + was closely correlated with Federation International of Gynecology and Obstetrics (FIGO) stage,histological grade and lymphnode metastasis (P ＜ 0.05) except for age (P ＞ 0.05).Conclusion Treg cytokines,HPV16 integration rate and severity of cervical lesions are positively correlated while Th17 cytokines show opposite effects.Th17/Treg cell-associated cytokines may play an important role in the occurrence and development of cervical cancer.
