中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
1期
51-54
,共4页
韩春雷%孟凡刚%潘剑%刘阿力%张凯%张建国
韓春雷%孟凡剛%潘劍%劉阿力%張凱%張建國
한춘뢰%맹범강%반검%류아력%장개%장건국
癫痫,颞叶%大鼠%基因表达谱%放射外科手术
癲癇,顳葉%大鼠%基因錶達譜%放射外科手術
전간,섭협%대서%기인표체보%방사외과수술
Epilepsy,temporal lobe%Rats%Gene expression profiling%Radiosurgery
目的 分析正常-癫痫-伽玛刀治疗过程中起核心调控作用的基因及其涉及的功能和信号转导途径,从基因表达水平探讨伽玛刀治疗癫痫的机制.方法 应用立体定向技术建立大鼠颞叶癫痫模型,进行伽玛刀照射,提取海马RNA进行芯片杂交、清洗、染色和扫描,获取芯片数据.基于芯片数据对正常-癫痫-伽玛刀治疗进行差异基因筛选,分析基因表达趋势,并对差异基因进行功能及信号转导通路分析,构建目标趋势的动态差异基因的共表达调控网络,从网络中得到正常-癫痫-伽玛刀治疗过程中起核心调控作用的基因群体.结果 按照1.5倍法筛选得到正常-癫痫-伽玛刀治疗3组间并集差异基因766个,这些差异基因涉及离子转运(P =6.85×10-24)、细胞黏附(P=1.55×10-8)、机械刺激反应(P =7.86×10-7)、钾离子转导(P =2.63×10-6)等生物功能及MAPK信号通路(P =5.55 ×10-6)、钙离子信号通路(P=4.29 ×10-5)、TGF-β信号通路(P＜0.01)等信号转导途径,共表达网络分析得到的核心调控基因包括Arf3、Akap5、Omd、Rtn4r等.结论 伽玛刀通过调节具有不同功能、介导不同信号转导途径的靶基因实现其对颞叶癫痫大鼠的治疗作用.
目的 分析正常-癲癇-伽瑪刀治療過程中起覈心調控作用的基因及其涉及的功能和信號轉導途徑,從基因錶達水平探討伽瑪刀治療癲癇的機製.方法 應用立體定嚮技術建立大鼠顳葉癲癇模型,進行伽瑪刀照射,提取海馬RNA進行芯片雜交、清洗、染色和掃描,穫取芯片數據.基于芯片數據對正常-癲癇-伽瑪刀治療進行差異基因篩選,分析基因錶達趨勢,併對差異基因進行功能及信號轉導通路分析,構建目標趨勢的動態差異基因的共錶達調控網絡,從網絡中得到正常-癲癇-伽瑪刀治療過程中起覈心調控作用的基因群體.結果 按照1.5倍法篩選得到正常-癲癇-伽瑪刀治療3組間併集差異基因766箇,這些差異基因涉及離子轉運(P =6.85×10-24)、細胞黏附(P=1.55×10-8)、機械刺激反應(P =7.86×10-7)、鉀離子轉導(P =2.63×10-6)等生物功能及MAPK信號通路(P =5.55 ×10-6)、鈣離子信號通路(P=4.29 ×10-5)、TGF-β信號通路(P＜0.01)等信號轉導途徑,共錶達網絡分析得到的覈心調控基因包括Arf3、Akap5、Omd、Rtn4r等.結論 伽瑪刀通過調節具有不同功能、介導不同信號轉導途徑的靶基因實現其對顳葉癲癇大鼠的治療作用.
목적 분석정상-전간-가마도치료과정중기핵심조공작용적기인급기섭급적공능화신호전도도경,종기인표체수평탐토가마도치료전간적궤제.방법 응용입체정향기술건립대서섭협전간모형,진행가마도조사,제취해마RNA진행심편잡교、청세、염색화소묘,획취심편수거.기우심편수거대정상-전간-가마도치료진행차이기인사선,분석기인표체추세,병대차이기인진행공능급신호전도통로분석,구건목표추세적동태차이기인적공표체조공망락,종망락중득도정상-전간-가마도치료과정중기핵심조공작용적기인군체.결과 안조1.5배법사선득도정상-전간-가마도치료3조간병집차이기인766개,저사차이기인섭급리자전운(P =6.85×10-24)、세포점부(P=1.55×10-8)、궤계자격반응(P =7.86×10-7)、갑리자전도(P =2.63×10-6)등생물공능급MAPK신호통로(P =5.55 ×10-6)、개리자신호통로(P=4.29 ×10-5)、TGF-β신호통로(P＜0.01)등신호전도도경,공표체망락분석득도적핵심조공기인포괄Arf3、Akap5、Omd、Rtn4r등.결론 가마도통과조절구유불동공능、개도불동신호전도도경적파기인실현기대섭협전간대서적치료작용.
Objective To explore the core controlling genes and their functions and pathways of gamma knife in the treatment of epilepsy in rats.Methods The temporal epilepsy rats induced by stereotactic technique were irradiated with gamma knife.Total RNA samples were isolated at 3 weeks postirradiation.After hybridization,washing and staining,the probe arrays were scanned to acquire the gene chip data.The functional categories and affected pathways of differentially regulated genes were analyzed.And the gene co-expression network was constructed to determine the core controlling genes.Results The differentiated genes of normal,epileptic and epileptic rats treated with gamma knife were screened by 1.5-fold method.There were a total of 766 union genes.The differentiated up-regulated and down-regulated genes were obtained.These genes were involved in functional categories such as ion transport (P =6.85 ×10-24),cell adhesion (P =1.55 × 10-8),response to mechanical stimulus (P =7.86 × 10-7),potassium ion transport (P =2.63 × 10-6) and such pathways as MAPK signaling (P =5.55 × 10-6),calcium signaling (P =4.29 × 10-5) and TGF-beta signaling (P ＜ 0.01),etc.And the core controlling genes from the gene co-expression network included Arf3,Akap5,Omd and Rtn4r,etc.Conclusion Gamma knife achieves its antiepileptic effect through modulating target genes involved in different functions and pathways.
