中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
2期
90-95
,共6页
彭飞%周建平%盛伟伟%张丹华%董明
彭飛%週建平%盛偉偉%張丹華%董明
팽비%주건평%성위위%장단화%동명
胰腺肿瘤%白血病抑制因子%Capan-2细胞株
胰腺腫瘤%白血病抑製因子%Capan-2細胞株
이선종류%백혈병억제인자%Capan-2세포주
Pancreatic neoplasms%Leukaemia inhibitory factor%Capan-2
目的 了解胰腺导管腺癌(PDAC)中白血病抑制因子(LIF)表达相关性及其临床病理学意义,并探讨白血病抑制因子对胰腺癌细胞生物学行为的影响.方法 应用免疫组织化学方法检测53例配对的PDAC及癌旁组织石蜡标本LIF蛋白表达水平,并分析LIF表达与患者临床病理学各项指标的关系,再以Western印迹法检测14对配对冷冻保存的新鲜PDAC和癌旁组织中LIF表达水平,然后运用Western印迹和聚合酶链反应(PCR)检测6株胰腺癌细胞系(AsPC-1、BxPC-3、PANC-1、SW-1990、Capan-2与Miapaca-2)中白血病抑制因子(LIF)蛋白和LIF-R mRNA表达水平,再运用MTT、Transwell实验检测LIF对胰腺癌细胞系增殖、侵袭及迁移的影响.结果 在53例胰腺癌组织中LIF的阳性表达率为66.0％ (35/53),在癌旁非肿瘤性胰腺导管中阳性表达率为35.8％(19/53);LIF在癌组织中表达明显高于配对的癌旁组织(66.0％与35.8％;t=3.031,P=0.004);LIF表达与肿瘤TNM分期(x2=3.635,P=0.057)、浸润深度(x2 =3.726,P =0.054)差异无统计学意义,但有明显的趋势效应.LIF在14对PDAC中表达水平同样高于癌旁组织(t=5.283,P＜0.01).免疫印迹和PCR显示:LIF因子在Capan-2中的表达水平相对低于其他5株细胞系,而其受体mRNA在Capan-2细胞中的表达水平相对高于其余5株细胞系.LIF因子能够促进Capan-2细胞的增殖(P＜0.05).并且能够增加Capan-2细胞的迁移及侵袭能力(P＜0.05).结论 LIF在胰腺癌高表达可能参与胰腺癌的发生进展,且在部分胰腺癌细胞系中,LIF能够促进胰腺癌细胞增殖、侵袭和迁移.
目的 瞭解胰腺導管腺癌(PDAC)中白血病抑製因子(LIF)錶達相關性及其臨床病理學意義,併探討白血病抑製因子對胰腺癌細胞生物學行為的影響.方法 應用免疫組織化學方法檢測53例配對的PDAC及癌徬組織石蠟標本LIF蛋白錶達水平,併分析LIF錶達與患者臨床病理學各項指標的關繫,再以Western印跡法檢測14對配對冷凍保存的新鮮PDAC和癌徬組織中LIF錶達水平,然後運用Western印跡和聚閤酶鏈反應(PCR)檢測6株胰腺癌細胞繫(AsPC-1、BxPC-3、PANC-1、SW-1990、Capan-2與Miapaca-2)中白血病抑製因子(LIF)蛋白和LIF-R mRNA錶達水平,再運用MTT、Transwell實驗檢測LIF對胰腺癌細胞繫增殖、侵襲及遷移的影響.結果 在53例胰腺癌組織中LIF的暘性錶達率為66.0％ (35/53),在癌徬非腫瘤性胰腺導管中暘性錶達率為35.8％(19/53);LIF在癌組織中錶達明顯高于配對的癌徬組織(66.0％與35.8％;t=3.031,P=0.004);LIF錶達與腫瘤TNM分期(x2=3.635,P=0.057)、浸潤深度(x2 =3.726,P =0.054)差異無統計學意義,但有明顯的趨勢效應.LIF在14對PDAC中錶達水平同樣高于癌徬組織(t=5.283,P＜0.01).免疫印跡和PCR顯示:LIF因子在Capan-2中的錶達水平相對低于其他5株細胞繫,而其受體mRNA在Capan-2細胞中的錶達水平相對高于其餘5株細胞繫.LIF因子能夠促進Capan-2細胞的增殖(P＜0.05).併且能夠增加Capan-2細胞的遷移及侵襲能力(P＜0.05).結論 LIF在胰腺癌高錶達可能參與胰腺癌的髮生進展,且在部分胰腺癌細胞繫中,LIF能夠促進胰腺癌細胞增殖、侵襲和遷移.
목적 료해이선도관선암(PDAC)중백혈병억제인자(LIF)표체상관성급기림상병이학의의,병탐토백혈병억제인자대이선암세포생물학행위적영향.방법 응용면역조직화학방법검측53례배대적PDAC급암방조직석사표본LIF단백표체수평,병분석LIF표체여환자림상병이학각항지표적관계,재이Western인적법검측14대배대냉동보존적신선PDAC화암방조직중LIF표체수평,연후운용Western인적화취합매련반응(PCR)검측6주이선암세포계(AsPC-1、BxPC-3、PANC-1、SW-1990、Capan-2여Miapaca-2)중백혈병억제인자(LIF)단백화LIF-R mRNA표체수평,재운용MTT、Transwell실험검측LIF대이선암세포계증식、침습급천이적영향.결과 재53례이선암조직중LIF적양성표체솔위66.0％ (35/53),재암방비종류성이선도관중양성표체솔위35.8％(19/53);LIF재암조직중표체명현고우배대적암방조직(66.0％여35.8％;t=3.031,P=0.004);LIF표체여종류TNM분기(x2=3.635,P=0.057)、침윤심도(x2 =3.726,P =0.054)차이무통계학의의,단유명현적추세효응.LIF재14대PDAC중표체수평동양고우암방조직(t=5.283,P＜0.01).면역인적화PCR현시:LIF인자재Capan-2중적표체수평상대저우기타5주세포계,이기수체mRNA재Capan-2세포중적표체수평상대고우기여5주세포계.LIF인자능구촉진Capan-2세포적증식(P＜0.05).병차능구증가Capan-2세포적천이급침습능력(P＜0.05).결론 LIF재이선암고표체가능삼여이선암적발생진전,차재부분이선암세포계중,LIF능구촉진이선암세포증식、침습화천이.
Objective To explore the expression of leukemia inhibitory factor (LIF) and its clinicopathological significance in human pancreatic ductal adenocarcinoma (PDAC) and examine its regulatory role of biological behaviors of pancreatic cancer cells.Methods The expression of LIF protein in 53 paired paraffin-embedded PDAC specimens and adjacent non-cancerous pancreatic tissues were detected by immunohistochemistry.The relationship between their expressions and clinicopathological characteristics was analyzed.Western bolt was used to examine the expression of LIF level in 14 paired fresh PDAC specimens and adjacent non-cancerous pancreatic tissues.Furthermore,Western blot and polymerase chain reaction (PCR) were used to detect the LIF-R mRNA and protein level of LIF in 6 pancreatic cancer cell lines (AsPC-1,BxPC-3,PANC-1,SW-1990,Capan-2 and Miapaca-2).And the assays of MTT,invasion and migration were used to detect the effects of LIF in regulating cell proliferation,invasion and migration in pancreatic cancer cell lines.Results The expression of LIF increased in 53 cases of PDAC versus paired normal tissues (66.0％ vs 35.8％ ; t =3.031,P =0.004).And its positive association with tumor TNM stage (x2 =3.635,P =0.057) and invasion depth (x2 =3.726,P =0.054) had no statistical significance.Univariate analysis revealed that LIF may be a correlative adverse prognostic indicator for patients with PDAC (x2 =3.233,P =0.072).The expression of LIF was much higher in 14 cases of PDAC than that in adjacent normal pancreatic tissues (t =5.283,P ＜ 0.01).The protein level of LIF was lower in Capan-2 cells than that in other 5 pancreatic cancer cell lines while mRNA level of LIF receptor was higher than that in other 5 cell lines.Also LIF could promote the proliferation,migration and invasion of Capan-2 cell (P ＜ 0.05).Conclusion An over-expression of LIF may contribute to the development and progression of PDAC.LIF can promote the proliferation,migration and invasion in some pancreatic cancer cells.
