CAJ | 학술논문

目的观察高脂血症叠加抑郁症大鼠的行为学及脑葡萄糖代谢.方法 30只SD大鼠按随机数字表法分为:对照组(CON)、高脂饮食组(HFD)、叠加模型组(DMF)各10只.HFD组以高脂饮食13周建立高脂血症动物模型;DMF组高脂饮食9周时,在此基础上采用慢性不可预见性轻度应激(CUMS)4周,建立高脂血症与抑郁症叠加模型.实验前、CUMS前后不同时点予血脂、糖水实验及脑葡萄糖代谢测定.结果 (1)血脂:9周时HFD、DMF两组大鼠血清总胆固醇[(2.67±0.04)、(2.68±0.02) mmol/L]、低密度脂蛋白[(1.08±0.03)、(1.06±0.01) mmol/L]明显高于CON组[(1.78±0.12)、(0.79 ±0.04)mmol/L,均P＜0.01],高密度脂蛋白[(0.89±0.04)、(0.87±0.03)mmol/L]明显低于CON组[(1.08±0.22) mmol/L,均P＜0.01].(2)糖水实验:与CON相比,HFD组9周时糖水相对消耗量差异无统计学意义(P =0.356),纯水相对消耗量、糖水偏好程度差异均有统计学意义(均P＜0.01);13周时HFD组纯水相对消耗量继续增多[(15.44±0.57)mg/kg,P＜0.01],糖水相对消耗量及糖水偏好程度均明显减少[(30.54±0.56) mg/kg,(74.37 ±0.66)％,均P＜0.01].与CON相比,DMF组CUMS期间各时点糖水相对消耗量均显著减少(均P＜0.01);对糖水的偏好程度逐渐降低(均P＜0.01).与HFD相比,DMF组13周时纯水、糖水相对消耗量及糖水偏好程度均明显减少(均P ＜0.01).(3)脑葡萄糖代谢:13周时,HFD组丘脑、纹状体脑葡萄糖代谢显著减低(均P＜0.01);DMF组下丘脑、岛叶皮质代谢显著增高,海马、内嗅皮质代谢显著降低(均P＜0.01).结论高脂血症与抑郁症叠加抑郁症状明显,对与情绪相关中枢葡萄糖代谢影响显著.
목적 관찰고지혈증첩가억욱증대서적행위학급뇌포도당대사.방법 30지SD대서안수궤수자표법분위:대조조(CON)、고지음식조(HFD)、첩가모형조(DMF)각10지.HFD조이고지음식13주건립고지혈증동물모형;DMF조고지음식9주시,재차기출상채용만성불가예견성경도응격(CUMS)4주,건립고지혈증여억욱증첩가모형.실험전、CUMS전후불동시점여혈지、당수실험급뇌포도당대사측정.결과 (1)혈지:9주시HFD、DMF량조대서혈청총담고순[(2.67±0.04)、(2.68±0.02) mmol/L]、저밀도지단백[(1.08±0.03)、(1.06±0.01) mmol/L]명현고우CON조[(1.78±0.12)、(0.79 ±0.04)mmol/L,균P＜0.01],고밀도지단백[(0.89±0.04)、(0.87±0.03)mmol/L]명현저우CON조[(1.08±0.22) mmol/L,균P＜0.01].(2)당수실험:여CON상비,HFD조9주시당수상대소모량차이무통계학의의(P =0.356),순수상대소모량、당수편호정도차이균유통계학의의(균P＜0.01);13주시HFD조순수상대소모량계속증다[(15.44±0.57)mg/kg,P＜0.01],당수상대소모량급당수편호정도균명현감소[(30.54±0.56) mg/kg,(74.37 ±0.66)％,균P＜0.01].여CON상비,DMF조CUMS기간각시점당수상대소모량균현저감소(균P＜0.01);대당수적편호정도축점강저(균P＜0.01).여HFD상비,DMF조13주시순수、당수상대소모량급당수편호정도균명현감소(균P ＜0.01).(3)뇌포도당대사:13주시,HFD조구뇌、문상체뇌포도당대사현저감저(균P＜0.01);DMF조하구뇌、도협피질대사현저증고,해마、내후피질대사현저강저(균P＜0.01).결론 고지혈증여억욱증첩가억욱증상명현,대여정서상관중추포도당대사영향현저.
Objective To explore the behaviors and brain glucose metabolism in a rat hyperlipidemia model with depression from chronic unpredictable mild stress (CUMS).Methods A total of 30 rats were randomly divided into control (CON),high-fat diet (HFD) and double model factor (DMF) groups (n =10 each).For HFD model,a high-lipid feed was provided for 13 weeks; for DMF model,a high-lipid feed for 9 weeks was followed by CUMS for 4 weeks.Serum lipids,sucrose test and brain glucose metabolism were individually measured at baseline,before and after CUMS.Results (1) Serum lipids:total cholesterol ((2.67 ± 0.04),(2.68 ± 0.02) mmol/L) and low density lipoprotein ((1.08 ± 0.03),(1.06 ±0.01) mmol/L) of HFD and DMF groups were both significantly higher than those of CON group ((1.78 ± 0.12),(0.79 ± 0.04) mmol/L,all P ＜ 0.01).However,high density lipoprutein ((0.89 ± 0.04),(0.87 ±0.03) mmol/L) of HFD and DMF groups was significantly lower than those of CON group ((1.08 ±0.22) mmol/L,all P ＜0.01) at week 9.(2) Sucrose test:As compared with CON group,there was no statistical significant difference in sucrose relative intake (P =0.356).However,the differences were statistically significant in relative water intake and sucrose preference (all P ＜ 0.01) at Week 9 in HFD group.At Week 13,water relative intake increased ((15.44 ± 0.57) mg/kg,P ＜ 0.01) and sucrose relative intake and sucrose preference significantly decreased in HFD group ((30.54 ± 0.56) mg/kg,(74.37 ± 0.66)％,all P ＜ 0.01).As compared with CON group,CUMS gradually reduced the relative intake of sucrose solution during CUMS periods in DMY rats (all P ＜0.01).As compared with HFD rats,water relative intake,sucrose relative intake and sucrose preference decreased in DMF group (all P ＜ 0.01).(3) Brain glucose metabolism:On 18F-fluorodeoxyglucose micro positron emission tomography,thalamus and striatum were deactivated in HFD group at Week 13 versus Week 9 (all P ＜0.01).Hypothalamus and insular cortex were activated while hippocampus and entorhinal cortex deactivated after CUMS in DMY group (all P ＜ 0.01).Conclusion When hyperlipidemia and depression co-exist,there are significant changes of brain glucose metabolism in many emotion-related brain regions.