中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
27期
2152-2155
,共4页
S100A10蛋白%月经周期%子宫内膜%标志物
S100A10蛋白%月經週期%子宮內膜%標誌物
S100A10단백%월경주기%자궁내막%표지물
S100A10 protein%Menstrual cycle%Endometrium%Biochemical marker
目的 观察S100A10在人类月经周期子宫内膜中的表达变化.方法 采用免疫组织化学、免疫印迹以及实时定量PCR检测人类月经周期子宫内膜中S100A10蛋白和基因的表达及定位.结果 40例人类子宫内膜标本结果显示,在增殖期S100A10蛋白的表达较弱,主要表达于子宫内膜腔上皮和腺上皮细胞的细胞质和细胞膜上,在基质细胞有散在表达;在分泌期的各时期,S100A10蛋白水平明显高于增殖期的各时期(2.26±0.17、3.75±0.22、2.65±0.20比1.17±0.11、1.32±0.14,均P＜0.05),并且在着床窗口期的表达呈现峰值;到分泌晚期略有下降(P＜0.05).基因检测结果显示,分泌期各期S100A10的mRNA的表达明显高于增殖期各期(0.50±0.05、1.02±0.10、0.55±0.05比0.25±0.02、0.30±0.03,均P＜0.05),在分泌中期出现表达峰值.结论 S100A10可能在胚胎植入中发挥作用,可以作为子宫内膜接受态的潜在标志物之一.
目的 觀察S100A10在人類月經週期子宮內膜中的錶達變化.方法 採用免疫組織化學、免疫印跡以及實時定量PCR檢測人類月經週期子宮內膜中S100A10蛋白和基因的錶達及定位.結果 40例人類子宮內膜標本結果顯示,在增殖期S100A10蛋白的錶達較弱,主要錶達于子宮內膜腔上皮和腺上皮細胞的細胞質和細胞膜上,在基質細胞有散在錶達;在分泌期的各時期,S100A10蛋白水平明顯高于增殖期的各時期(2.26±0.17、3.75±0.22、2.65±0.20比1.17±0.11、1.32±0.14,均P＜0.05),併且在著床窗口期的錶達呈現峰值;到分泌晚期略有下降(P＜0.05).基因檢測結果顯示,分泌期各期S100A10的mRNA的錶達明顯高于增殖期各期(0.50±0.05、1.02±0.10、0.55±0.05比0.25±0.02、0.30±0.03,均P＜0.05),在分泌中期齣現錶達峰值.結論 S100A10可能在胚胎植入中髮揮作用,可以作為子宮內膜接受態的潛在標誌物之一.
목적 관찰S100A10재인류월경주기자궁내막중적표체변화.방법 채용면역조직화학、면역인적이급실시정량PCR검측인류월경주기자궁내막중S100A10단백화기인적표체급정위.결과 40례인류자궁내막표본결과현시,재증식기S100A10단백적표체교약,주요표체우자궁내막강상피화선상피세포적세포질화세포막상,재기질세포유산재표체;재분비기적각시기,S100A10단백수평명현고우증식기적각시기(2.26±0.17、3.75±0.22、2.65±0.20비1.17±0.11、1.32±0.14,균P＜0.05),병차재착상창구기적표체정현봉치;도분비만기략유하강(P＜0.05).기인검측결과현시,분비기각기S100A10적mRNA적표체명현고우증식기각기(0.50±0.05、1.02±0.10、0.55±0.05비0.25±0.02、0.30±0.03,균P＜0.05),재분비중기출현표체봉치.결론 S100A10가능재배태식입중발휘작용,가이작위자궁내막접수태적잠재표지물지일.
Objective To explore the cyclic changes of S100A10 expression in human endometrium.Methods The gene and protein expression of S100A10 in human endometrium was examined by real-time polymerase chain reaction (PCR),immunohistochemical staining and Western blot.Results S100A10 protein was predominantly expressed in cytoplasm and cytomembrane of luminal and glandular epithelia.The S100A10 protein was continuously expressed from proliferative phase to secretory phase of menstrual cycle.However the expression in the secretory phase was much higher than in the proliferative phase (2.26 ±0.17,3.75 ±0.22,2.65 ±0.20 vs 1.17 ±0.11,1.32 ±0.14,both P ＜0.05).Specifically,the highest level of S100A10 expression occurred during the mid-secretory phase.Then realtime PCR was used to analyze the mRNA expression of S100A10 in human endometrial samples at various points during the menstrual cycle.And the results confirmed the corresponding changes at the genetic level (0.50 ±0.05,1.02 ±0.10,0.55 ±0.05 vs 0.25 ±0.02,0.30 ±0.03,both P ＜0.05).Conclusion The endometrial expression of S100A10 increases during the window of embryo implantation.It suggests that it may increase the endometrial receptivity of uterine epithelial cells for embryos.
