中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
28期
2193-2196
,共4页
郭贺%闫文姬%杨云生%郭明洲
郭賀%閆文姬%楊雲生%郭明洲
곽하%염문희%양운생%곽명주
胃肿瘤%DNA甲基化%DNA修复
胃腫瘤%DNA甲基化%DNA脩複
위종류%DNA갑기화%DNA수복
Gastric neoplasms%DNA methylation%DNA repair
目的 联合检测7个DNA损伤修复基因在胃癌组织中的异常甲基化改变.方法 收集解放军总医院胃癌手术标本70例,酚-氯仿法提取DNA后,经亚硫酸氢盐处理,甲基化特异性聚合酶链反应(MSP)方法检测标本中MLH1,CHFR,MGMT,FANCF,Rassf1A,BRCA1和GSTpi基因启动子区CpG岛的甲基化状况.结果 7个DNA损伤修复基因的甲基化率分别如下:MLH1 22.9％(16/70),CHFR 47.1％ (33/70),MGMT 34.3％ (24/70),FANCF 11.4％(8/70),RASSF1A 7.1％(5/70),BRCA1 1.4％ (1/70),GSTpi 0％ (0/70).临床资料分析显示CHFR的甲基化与患者年龄、胃癌肿瘤大小和分化程度相关.基因间相关性分析显示CHFR的甲基化分别与MLH1和MGMT基因的甲基化相关.结论 MLH1,CHFR和MGMT基因在胃癌组织中频繁甲基化,而FANCF、RASSF1A、BRCA1和GSTpi基因在胃癌组织中甲基化率低,MLH1,CHFR和MGMT基因在胃癌的发生发展中可能起重要作用.联合检测上述3个基因的甲基化可能对胃癌的诊断提供帮助.
目的 聯閤檢測7箇DNA損傷脩複基因在胃癌組織中的異常甲基化改變.方法 收集解放軍總醫院胃癌手術標本70例,酚-氯倣法提取DNA後,經亞硫痠氫鹽處理,甲基化特異性聚閤酶鏈反應(MSP)方法檢測標本中MLH1,CHFR,MGMT,FANCF,Rassf1A,BRCA1和GSTpi基因啟動子區CpG島的甲基化狀況.結果 7箇DNA損傷脩複基因的甲基化率分彆如下:MLH1 22.9％(16/70),CHFR 47.1％ (33/70),MGMT 34.3％ (24/70),FANCF 11.4％(8/70),RASSF1A 7.1％(5/70),BRCA1 1.4％ (1/70),GSTpi 0％ (0/70).臨床資料分析顯示CHFR的甲基化與患者年齡、胃癌腫瘤大小和分化程度相關.基因間相關性分析顯示CHFR的甲基化分彆與MLH1和MGMT基因的甲基化相關.結論 MLH1,CHFR和MGMT基因在胃癌組織中頻繁甲基化,而FANCF、RASSF1A、BRCA1和GSTpi基因在胃癌組織中甲基化率低,MLH1,CHFR和MGMT基因在胃癌的髮生髮展中可能起重要作用.聯閤檢測上述3箇基因的甲基化可能對胃癌的診斷提供幫助.
목적 연합검측7개DNA손상수복기인재위암조직중적이상갑기화개변.방법 수집해방군총의원위암수술표본70례,분-록방법제취DNA후,경아류산경염처리,갑기화특이성취합매련반응(MSP)방법검측표본중MLH1,CHFR,MGMT,FANCF,Rassf1A,BRCA1화GSTpi기인계동자구CpG도적갑기화상황.결과 7개DNA손상수복기인적갑기화솔분별여하:MLH1 22.9％(16/70),CHFR 47.1％ (33/70),MGMT 34.3％ (24/70),FANCF 11.4％(8/70),RASSF1A 7.1％(5/70),BRCA1 1.4％ (1/70),GSTpi 0％ (0/70).림상자료분석현시CHFR적갑기화여환자년령、위암종류대소화분화정도상관.기인간상관성분석현시CHFR적갑기화분별여MLH1화MGMT기인적갑기화상관.결론 MLH1,CHFR화MGMT기인재위암조직중빈번갑기화,이FANCF、RASSF1A、BRCA1화GSTpi기인재위암조직중갑기화솔저,MLH1,CHFR화MGMT기인재위암적발생발전중가능기중요작용.연합검측상술3개기인적갑기화가능대위암적진단제공방조.
Objective To detect the status of promoter region methylation of damage repair genes in gastric cancer and analyze its association with clinicopathologic characteristics.Methods A total of 70 human gastric cancer tissue samples representing all stages of disease were obtained from surgical resection specimens.DNA was extracted with the phenol-chloroform method.The technique of methylation specific polymerase chain reaction (MSP) was used to examine the methylation status of MLH1,CHFR,MGMT,FANCF,Rassf1A,BRCA1 and GSTpi in gastric cancer specimens.And MSP products were analyzed with 2％ agarose gel electrophoresis.Results The methylation rates of DNA damage related genes in human gastric cancer were as follows:MLH1 22.9％ (16/70),CHFR 47.1％ (33/70),MGMT 34.3％ (24/70),FANCF 11.4％ (8/70),RASSF1A 7.1％ (5/70),BRCA1 1.4％ (1/70) and GSTpi0％ (0/70).And CHFR methylation was correlated with patient age (＜ 60 year) (P =0.035),tumor size (diameter ≥5 cm) (P =0.031) and low differentiation (P =0.019).In addition,CHFR methylation was associated with MLH1 methylation and MGMT methylation respectively.Conclusion In human gastric cancer,the DNA damage repair genes of MLH1,CHFR and MGMT are frequently methylated while FANCF,RASSF1A,BRCA1 and GSTpi infrequently.Thus MLH1,CHFR and MGMT may play important roles during carcinogenesis of gastric cancer.And these methylated genes may serve as potential detection markers for gastric cancer.
