CAJ | 학술논문

目的探讨早期香烟烟雾暴露对哮喘小鼠T细胞亚群功能的影响.方法 40只20日龄BALB/c小鼠按随机数字表法随机分为4组:空气对照组、烟雾对照组,空气+致敏组、烟雾+致敏组,每组10只.其中烟雾对照组和烟雾+致敏组给予香烟烟雾暴露3周,其他2组暴露于空气中作对照;8周龄时,空气+致敏组和烟雾+致敏组给予卵清蛋白(OVA)致敏建立小鼠哮喘模型,其他2组给予生理盐水作对照.通过流式细胞术检测各组脾单个核细胞中T细胞亚群调节性T细胞(Treg)和效应性T细胞(Te)亚群包括辅助性T细胞Th1、Th2、Th17的百分比,酶联免疫吸附(ELISA)法检测支气管肺泡灌洗液(BALF)中转化生长因子(TGF)-β、白细胞介素(IL)-4、IL-5、IL-10、IL-13、IL-17A和γ干扰素(IFN-γ)的含量,并比较各组脾单个核细胞中Treg与Te百分比的比值.结果烟雾+致敏组脾单个核细胞中的Th1、Th2、Th17、Treg百分比分别为(21.47±2.84)％、(16.33±3.02)％、(1.89±0.23)％、(2.92 ±0.40)％,均显著高于空气+致敏组的(14.78±2.21)％、(9.72±1.46)％、(1.01 ±0.12)％、(1.84±0.22)％,BALF中IL-4、IL-5、IL-10、IL-13、IL-17A、IFN-γ、TGF-β含量分别为(138.6±18.9)、(195.3 ±32.6)、(22.8±3.9)、(716.9±123.6)、(62.8±7.9)、(173.2±25.5)、(66.5±8.2) ng/L,也均显著高于空气+致敏组的(90.1±13.7)、(128.5±21.8)、(14.4±2.9)、(421.4 ±60.4)、(40.4±6.2)、(113.8±16.9)、(42.9±3.8)ng/L(均P＜0.05);但空气+致敏组与烟雾+致敏组脾单个核细胞中的Treg与Te百分比比值差异无统计学意义(P＞0.05).结论早期香烟烟雾暴露可显著加剧哮喘小鼠T细胞亚群功能的紊乱.
목적 탐토조기향연연무폭로대효천소서T세포아군공능적영향.방법 40지20일령BALB/c소서안수궤수자표법수궤분위4조:공기대조조、연무대조조,공기+치민조、연무+치민조,매조10지.기중연무대조조화연무+치민조급여향연연무폭로3주,기타2조폭로우공기중작대조;8주령시,공기+치민조화연무+치민조급여란청단백(OVA)치민건립소서효천모형,기타2조급여생리염수작대조.통과류식세포술검측각조비단개핵세포중T세포아군조절성T세포(Treg)화효응성T세포(Te)아군포괄보조성T세포Th1、Th2、Th17적백분비,매련면역흡부(ELISA)법검측지기관폐포관세액(BALF)중전화생장인자(TGF)-β、백세포개소(IL)-4、IL-5、IL-10、IL-13、IL-17A화γ간우소(IFN-γ)적함량,병비교각조비단개핵세포중Treg여Te백분비적비치.결과 연무+치민조비단개핵세포중적Th1、Th2、Th17、Treg백분비분별위(21.47±2.84)％、(16.33±3.02)％、(1.89±0.23)％、(2.92 ±0.40)％,균현저고우공기+치민조적(14.78±2.21)％、(9.72±1.46)％、(1.01 ±0.12)％、(1.84±0.22)％,BALF중IL-4、IL-5、IL-10、IL-13、IL-17A、IFN-γ、TGF-β함량분별위(138.6±18.9)、(195.3 ±32.6)、(22.8±3.9)、(716.9±123.6)、(62.8±7.9)、(173.2±25.5)、(66.5±8.2) ng/L,야균현저고우공기+치민조적(90.1±13.7)、(128.5±21.8)、(14.4±2.9)、(421.4 ±60.4)、(40.4±6.2)、(113.8±16.9)、(42.9±3.8)ng/L(균P＜0.05);단공기+치민조여연무+치민조비단개핵세포중적Treg여Te백분비비치차이무통계학의의(P＞0.05).결론 조기향연연무폭로가현저가극효천소서T세포아군공능적문란.
Objective To explore the effects of early cigarette smoke exposure on the immune functions of T-lymphocyte subsets in asthmatic mice model.Methods Forty mice (20 days) were randomly allocated into four groups:air control group,cigarette smoke (CS) control group,air + ovalbumin (OVA) group,and CS + OVA group (n =10 each).The CS control and CS + OVA groups were exposed to cigarette smoke for 3 weeks while the other two groups exposed to air.At an age of 8 weeks,air + OVA and CS + OVA groups had OVA sensitization for establishing an asthma model while the other two groups were selected as control.The percentage of regulatory T cells (Treg),effect of T cell (Te) subgroup including T helper type (Th)1,Th2 and Th17 in splenic mononuclear T cell were determined by flow cytometry.The levels of transforming growth factor-β (TGF-β),interleukin (IL)-4,IL-5,IL-10,IL-13,IL-17A and interferon-γ (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA).And the percentage ratios of Treg to Te were compared among four groups.Results The percentages of Th1,Th2,Th17 and Treg in splenic mononuclear of CS + OVA group ((21.47 ±2.84)％,(16.33 ± 3.02) ％,(1.89 ± 0.23) ％ and (2.92 ± 0.40) ％,respectively) were all significantly higher than those in air + OVA group ((14.78 ± 2.21) ％,(9.72 ± 1.46) ％,(1.01 ± 0.12) ％ and (1.84 ± 0.22)％,respectively),the levels of IL-4,IL-5,IL-10,IL-13,IL-17A,IFN-γand TGF-β in BALF of CS+OVA group ((138.6±18.9),(195.3 ±32.6),(22.8 ±3.9),(716.9 ±123.6),(62.8 ±7.9),(173.2 ± 25.5) and (66.5 ± 8.2) ng/L,respectively) were all significantly higher than those in air + OVA group ((90.1±13.7),(128.5 ±21.8),(14.4±2.9),(421.4 ±60.4),(40.4±6.2),(113.8 ± 16.9) and (42.9 ± 3.8) ng/L,respectively) (all P ＜ 0.05).No significant difference of Treg/Te percentage ratio existed in mice spleen mononuclear cells between air + OVA group and CS + OVA group (P ＞0.05).Conclusion Early cigarette smoke exposure could significantly exacerbate T cell immune dysfunction in asthmatic mice.