中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
28期
2222-2226
,共5页
毕燕琳%王彬%尹曾%张高峰%陈怀龙%王明山
畢燕琳%王彬%尹曾%張高峰%陳懷龍%王明山
필연림%왕빈%윤증%장고봉%진부룡%왕명산
蛋白质丝氨酸苏氨酸激酶%星状神经节%神经传导阻滞%星形细胞
蛋白質絲氨痠囌氨痠激酶%星狀神經節%神經傳導阻滯%星形細胞
단백질사안산소안산격매%성상신경절%신경전도조체%성형세포
Protein-serine-threonine kinases%Stellate ganglion%Nerve block%Astrocytes
目的 探讨星状神经节阻滞(SGB)对老龄大鼠术后海马神经元腺苷酸活化蛋白激酶和星形胶质细胞的影响.方法 健康雄性老龄SD大鼠96只,月龄18 ~20个月,体重450 ~ 550 g,采用随机数字表法,将其随机分为4组:空白对照组(Ⅰ组)、手术组(Ⅱ组)、生理盐水+手术组(Ⅲ组)、SGB+手术组(Ⅳ组).SGB组注射0.25%布比卡因0.15 ml行右侧SGB,给药结束后15 min,Ⅱ组、Ⅲ组和Ⅳ组实施手术.每组分别于术后1、3和5d随机处死8只大鼠.采用Morris水迷宫法记录大鼠逃避潜伏期和游泳路径;采用反转录PCR(RT-PCR)和Western印迹法分别测定大鼠海马内腺苷酸活化激酶(AMPK)mRNA和AMPK、p-AMPK的表达.采用免疫组织化学法计数胶质纤维酸性蛋白(GFAP)阳性细胞总个数.结果 与Ⅰ组比较,Ⅱ组、Ⅲ组、Ⅳ组大鼠逃避潜伏期和游泳路径延长,海马AMPK mRNA、AMPK、p-AMPK表达上调,GFAP表达细胞数量增多;与Ⅱ组、Ⅲ组比较,Ⅳ组大鼠逃避潜伏期和游泳路径缩短,海马AMPK mRNA、AMPK、p-AMPK表达下调,GFAP表达细胞数量减少.结论 SGB可改善老龄大鼠术后认知功能,其机制可能与下调海马神经元腺苷酸活化蛋白激酶和抑制星形胶质细胞激活有关.
目的 探討星狀神經節阻滯(SGB)對老齡大鼠術後海馬神經元腺苷痠活化蛋白激酶和星形膠質細胞的影響.方法 健康雄性老齡SD大鼠96隻,月齡18 ~20箇月,體重450 ~ 550 g,採用隨機數字錶法,將其隨機分為4組:空白對照組(Ⅰ組)、手術組(Ⅱ組)、生理鹽水+手術組(Ⅲ組)、SGB+手術組(Ⅳ組).SGB組註射0.25%佈比卡因0.15 ml行右側SGB,給藥結束後15 min,Ⅱ組、Ⅲ組和Ⅳ組實施手術.每組分彆于術後1、3和5d隨機處死8隻大鼠.採用Morris水迷宮法記錄大鼠逃避潛伏期和遊泳路徑;採用反轉錄PCR(RT-PCR)和Western印跡法分彆測定大鼠海馬內腺苷痠活化激酶(AMPK)mRNA和AMPK、p-AMPK的錶達.採用免疫組織化學法計數膠質纖維痠性蛋白(GFAP)暘性細胞總箇數.結果 與Ⅰ組比較,Ⅱ組、Ⅲ組、Ⅳ組大鼠逃避潛伏期和遊泳路徑延長,海馬AMPK mRNA、AMPK、p-AMPK錶達上調,GFAP錶達細胞數量增多;與Ⅱ組、Ⅲ組比較,Ⅳ組大鼠逃避潛伏期和遊泳路徑縮短,海馬AMPK mRNA、AMPK、p-AMPK錶達下調,GFAP錶達細胞數量減少.結論 SGB可改善老齡大鼠術後認知功能,其機製可能與下調海馬神經元腺苷痠活化蛋白激酶和抑製星形膠質細胞激活有關.
목적 탐토성상신경절조체(SGB)대노령대서술후해마신경원선감산활화단백격매화성형효질세포적영향.방법 건강웅성노령SD대서96지,월령18 ~20개월,체중450 ~ 550 g,채용수궤수자표법,장기수궤분위4조:공백대조조(Ⅰ조)、수술조(Ⅱ조)、생리염수+수술조(Ⅲ조)、SGB+수술조(Ⅳ조).SGB조주사0.25%포비잡인0.15 ml행우측SGB,급약결속후15 min,Ⅱ조、Ⅲ조화Ⅳ조실시수술.매조분별우술후1、3화5d수궤처사8지대서.채용Morris수미궁법기록대서도피잠복기화유영로경;채용반전록PCR(RT-PCR)화Western인적법분별측정대서해마내선감산활화격매(AMPK)mRNA화AMPK、p-AMPK적표체.채용면역조직화학법계수효질섬유산성단백(GFAP)양성세포총개수.결과 여Ⅰ조비교,Ⅱ조、Ⅲ조、Ⅳ조대서도피잠복기화유영로경연장,해마AMPK mRNA、AMPK、p-AMPK표체상조,GFAP표체세포수량증다;여Ⅱ조、Ⅲ조비교,Ⅳ조대서도피잠복기화유영로경축단,해마AMPK mRNA、AMPK、p-AMPK표체하조,GFAP표체세포수량감소.결론 SGB가개선노령대서술후인지공능,기궤제가능여하조해마신경원선감산활화단백격매화억제성형효질세포격활유관.
Objective To investigate the effects of stellate ganglion block on AMP-ativated protein kinase and astrocyte in hippocampal neurons in postoperative aged rats.Methods A total of 96 male Sprague-Dawley rats aged 18-20 months,weighing 450-550 g,were randomly divided into 4 groups (n =24):Control group (Group Ⅰ) ; Surgery group (Group Ⅱ) ; Normal saline + surgery group (Group Ⅲ) ;SGB + surgery group(GroupⅣ).SGB with 0.25% bupivacaine 0.15 ml and operation was conducted after 15 rmin in groups Ⅱ,Ⅲ and Ⅳ.The eight rats were randomly sacrificed in each group postoperative 1,3,and 5 d.The escape latency and swimming distance were recorded by morris water maze.The expression of AMPKmRNA,AMPK and phosphorylated AMPK (p-AMPK) was measured by RT-PCR and Western blot.The expression of GFAP was measured by immunohistochemisty.Results Compared with Ⅰ group,the escape latency and swimming distance were significantly prolonged and the expression of AMPK mRNA,AMPK and p-AMPKwas up-regulated and the expression of GFAP in astrocyte was significatively increased in groups Ⅱ,Ⅲ and Ⅳ.Compared with Ⅱ and Ⅲ group,the escape latency and swimming distance were significantly shorten and the expression of AMPK mRNA,AMPK and p-AMPKwas down-regulated and the expression of GFAP in astrocyte was significatively decreased in group Ⅳ.Conclusion SGB can improve the postoperative cognitive function,and the mechanism may be associated with down-regulating the expression of AMPK and restraining the activation of the astrocyte.