中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
29期
2300-2303
,共4页
楚喜英%宋玉霞%万利静%谭丽
楚喜英%宋玉霞%萬利靜%譚麗
초희영%송옥하%만리정%담려
子宫内膜%白血病抑制因子%表皮生长因子%超排卵%坤泰胶囊
子宮內膜%白血病抑製因子%錶皮生長因子%超排卵%坤泰膠囊
자궁내막%백혈병억제인자%표피생장인자%초배란%곤태효낭
Endometrium%LIF%EGF%Superovulation%Kuntai Capsules
目的 探讨坤泰胶囊对控制性超排卵小鼠子宫内膜厚度及白血病抑制因子(LIF)、表皮生长因子(EGF)蛋白表达的影响.方法 将健康昆明(SD)小鼠随机分成4组,每组15只,即:对照组(A组)、控制性超排卵组[COH组(B组)]、COH+常规剂量坤泰胶囊组(C组)、COH+大剂量坤泰胶囊组[常规剂量2倍(D组)].建立COH模型.利用多功能图像分析仪测量子宫内膜厚度,采用免疫组化法检测子宫内膜LIF、EGF蛋白的表达.结果 (1)C组(413±5) μm、D组(415 ±6) μm小鼠子宫内膜厚度均＞A组(385 ±4) μm、B组(388±6) μm,差异有统计学意义(P＜0.05).(2)LIF蛋白在小鼠增殖期子宫内膜的表达水平显著低于分泌期,EGF、LIF蛋白在B组小鼠子宫内膜的表达水平显著低于A组,在C组和D组的表达均高于B组,且在D组的表达水平高于C组,差异有统计学意义(P＜0.05).结论 坤泰胶囊可促进小鼠子宫内膜增长,提高控制性超排卵子宫内膜中EGF、LIF蛋白的表达水平,从而改善子宫内膜的微环境,以提高子宫内膜的容受性,而随着坤泰胶囊剂量的增加,对子宫内膜的改善作用更加明显.
目的 探討坤泰膠囊對控製性超排卵小鼠子宮內膜厚度及白血病抑製因子(LIF)、錶皮生長因子(EGF)蛋白錶達的影響.方法 將健康昆明(SD)小鼠隨機分成4組,每組15隻,即:對照組(A組)、控製性超排卵組[COH組(B組)]、COH+常規劑量坤泰膠囊組(C組)、COH+大劑量坤泰膠囊組[常規劑量2倍(D組)].建立COH模型.利用多功能圖像分析儀測量子宮內膜厚度,採用免疫組化法檢測子宮內膜LIF、EGF蛋白的錶達.結果 (1)C組(413±5) μm、D組(415 ±6) μm小鼠子宮內膜厚度均＞A組(385 ±4) μm、B組(388±6) μm,差異有統計學意義(P＜0.05).(2)LIF蛋白在小鼠增殖期子宮內膜的錶達水平顯著低于分泌期,EGF、LIF蛋白在B組小鼠子宮內膜的錶達水平顯著低于A組,在C組和D組的錶達均高于B組,且在D組的錶達水平高于C組,差異有統計學意義(P＜0.05).結論 坤泰膠囊可促進小鼠子宮內膜增長,提高控製性超排卵子宮內膜中EGF、LIF蛋白的錶達水平,從而改善子宮內膜的微環境,以提高子宮內膜的容受性,而隨著坤泰膠囊劑量的增加,對子宮內膜的改善作用更加明顯.
목적 탐토곤태효낭대공제성초배란소서자궁내막후도급백혈병억제인자(LIF)、표피생장인자(EGF)단백표체적영향.방법 장건강곤명(SD)소서수궤분성4조,매조15지,즉:대조조(A조)、공제성초배란조[COH조(B조)]、COH+상규제량곤태효낭조(C조)、COH+대제량곤태효낭조[상규제량2배(D조)].건립COH모형.이용다공능도상분석의측양자궁내막후도,채용면역조화법검측자궁내막LIF、EGF단백적표체.결과 (1)C조(413±5) μm、D조(415 ±6) μm소서자궁내막후도균＞A조(385 ±4) μm、B조(388±6) μm,차이유통계학의의(P＜0.05).(2)LIF단백재소서증식기자궁내막적표체수평현저저우분비기,EGF、LIF단백재B조소서자궁내막적표체수평현저저우A조,재C조화D조적표체균고우B조,차재D조적표체수평고우C조,차이유통계학의의(P＜0.05).결론 곤태효낭가촉진소서자궁내막증장,제고공제성초배란자궁내막중EGF、LIF단백적표체수평,종이개선자궁내막적미배경,이제고자궁내막적용수성,이수착곤태효낭제량적증가,대자궁내막적개선작용경가명현.
Objective To explore the effects of Kuntai Capsules on endometrial thickness and the expressions of leukemia inhibitory factor (LIF) and epidermal growth factor (EGF) in mouse after controlled ovarian hyperstimulation.Methods Healthy Sprague-Dawley mice were randomly allocated into 4 groups of control (group A),controlled ovarian hyperstimulation [COH (group B)],COH plus low-dose Kuntai Capsules (group C) and COH plus large-dose Kuntai Capsules [2x low-dose (group D)].The controlled ovarian hyperstimulation model was established.The endometrial thickness was measured by computerized multi-functional image analyzer.And the expressions of LIF and EGF in proliferating endometrium were examined by immunohistochemistry.Results The endometrial thickness of groups C and D were higher than that of groups A and B.And there were significant differences (P ＜ 0.05).The expression levels of LIF protein in proliferating endometrium was weaker than implantation window phase,the expression levels of EGF and LIF in group B was weaker than group A,groups C and D stronger than groups B and D was stronger than group C.And there were significant differences (P ＜ 0.05).Conclusion Kuntai Capsules can promote the growth of endometrium and enhance the expression levels of EGF and LIF in mice.And it may improve the ability of endometrial receptivity through optimized microenvironment.And a larger dose of Kuntai Capsules yields better outcomes.
