中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
30期
2382-2385
,共4页
殷德涛%许建辉%王勇飞%李红强%张艳%江金花%王庆端
慇德濤%許建輝%王勇飛%李紅彊%張豔%江金花%王慶耑
은덕도%허건휘%왕용비%리홍강%장염%강금화%왕경단
甲状腺肿瘤%癌,乳头状%CCDC67%RT-PCR
甲狀腺腫瘤%癌,乳頭狀%CCDC67%RT-PCR
갑상선종류%암,유두상%CCDC67%RT-PCR
Thyroid neoplasm%Carcinoma,papillary%CCDC67%Reverse transcriptionpolymerase chain reaction
目的 探讨甲状腺乳头状癌(PTC)及癌旁组织中CCDC67基因转录水平的差异及其与PTC临床病理特征的关系.方法 采用逆转录聚合酶链反应(RT-PCR)方法,检测了48例PTC及其相对应的癌旁组织中CCDC67的转录情况;同时对甲状腺癌细胞系(TPC-1、SW579)CCDC67基因的转录进行了检测.结果 在TPC-1、SW579细胞系中,CCDC67mRNA表达均缺失.癌旁组织中均出现CCDC67mRNA的表达(100.0％,48/48),而在PTC中CCDC67基因mRNA表达明显下降为39.6％(19/48) (P＜0.05).在PTC中,Ⅰ～Ⅱ期与Ⅲ～Ⅳ期的CCDC67mRNA表达率分别为60％ (12/20)、25％ (7/28);在有淋巴结转移的PTC中CCDC67mRNA的表达率为24.1％ (7/29),在无淋巴结转移的PTC中CCDC67mRNA的表达率为63.2％ (12/19);在病理分级Ⅰ级和Ⅱ级中,CCDC67mRNA的表达率分别为61.1％(11/18)和26.7％(8/30).以上各组中差异均有统计学意义(均P＜0.05).结论 CCDC67mRNA表达的异常可能是引起CCDC67失活的原因,并可能与PTC的发生、发展及转移有关.
目的 探討甲狀腺乳頭狀癌(PTC)及癌徬組織中CCDC67基因轉錄水平的差異及其與PTC臨床病理特徵的關繫.方法 採用逆轉錄聚閤酶鏈反應(RT-PCR)方法,檢測瞭48例PTC及其相對應的癌徬組織中CCDC67的轉錄情況;同時對甲狀腺癌細胞繫(TPC-1、SW579)CCDC67基因的轉錄進行瞭檢測.結果 在TPC-1、SW579細胞繫中,CCDC67mRNA錶達均缺失.癌徬組織中均齣現CCDC67mRNA的錶達(100.0％,48/48),而在PTC中CCDC67基因mRNA錶達明顯下降為39.6％(19/48) (P＜0.05).在PTC中,Ⅰ～Ⅱ期與Ⅲ～Ⅳ期的CCDC67mRNA錶達率分彆為60％ (12/20)、25％ (7/28);在有淋巴結轉移的PTC中CCDC67mRNA的錶達率為24.1％ (7/29),在無淋巴結轉移的PTC中CCDC67mRNA的錶達率為63.2％ (12/19);在病理分級Ⅰ級和Ⅱ級中,CCDC67mRNA的錶達率分彆為61.1％(11/18)和26.7％(8/30).以上各組中差異均有統計學意義(均P＜0.05).結論 CCDC67mRNA錶達的異常可能是引起CCDC67失活的原因,併可能與PTC的髮生、髮展及轉移有關.
목적 탐토갑상선유두상암(PTC)급암방조직중CCDC67기인전록수평적차이급기여PTC림상병리특정적관계.방법 채용역전록취합매련반응(RT-PCR)방법,검측료48례PTC급기상대응적암방조직중CCDC67적전록정황;동시대갑상선암세포계(TPC-1、SW579)CCDC67기인적전록진행료검측.결과 재TPC-1、SW579세포계중,CCDC67mRNA표체균결실.암방조직중균출현CCDC67mRNA적표체(100.0％,48/48),이재PTC중CCDC67기인mRNA표체명현하강위39.6％(19/48) (P＜0.05).재PTC중,Ⅰ～Ⅱ기여Ⅲ～Ⅳ기적CCDC67mRNA표체솔분별위60％ (12/20)、25％ (7/28);재유림파결전이적PTC중CCDC67mRNA적표체솔위24.1％ (7/29),재무림파결전이적PTC중CCDC67mRNA적표체솔위63.2％ (12/19);재병리분급Ⅰ급화Ⅱ급중,CCDC67mRNA적표체솔분별위61.1％(11/18)화26.7％(8/30).이상각조중차이균유통계학의의(균P＜0.05).결론 CCDC67mRNA표체적이상가능시인기CCDC67실활적원인,병가능여PTC적발생、발전급전이유관.
Objective To explore the expression of CCDC67 mRNA between papillary thyroid carcinoma (PTC) and adjacent normal tissues.Methods Reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect the expression of CCDC67 mRNA in 48 cases of papillary thyroid carcinoma and their matched adjacent non-cancerous epithelium and cell lines of thyroid carcinoma.Results There was no expression of CCDC67 mRNA in SW579 and TPC-1 cell line.All cases had the expression of CCDC67 in adjacent normal tissues while the expression of CCDC67 mRNA was down-regulated significantly,only 39.6％ (19/48) in 48 cases of thyroid carcinoma.In PTC,the expression rates of CCDC67 mRNA in stage Ⅰ-Ⅱ and Ⅲ-Ⅳ were 60％ (12/20) and 25％ (7/28) and 24.1％ (7/29) and 63.2％ (12/19) in the lymph node metastasis and non-metastasis groups respectively.Also the expression rates of CCDC67 mRNA in Grades Ⅰ and Ⅱ were 61.1％ (11/18) and 26.7％ (8/30) respectively.And there was significant difference between two groups (P ＜ 0.05).Conclusions The down-regulated expression of CCDC67 is one of reasons for inactivation of PTC.And it may play an important role in carcinogenesis and progression of PTC.
