中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
35期
2754-2756
,共3页
吴日乐%许孝飞%刘爱华%李文辉%彭汤明%钱增辉%吴京%康慧斌%吴中学
吳日樂%許孝飛%劉愛華%李文輝%彭湯明%錢增輝%吳京%康慧斌%吳中學
오일악%허효비%류애화%리문휘%팽탕명%전증휘%오경%강혜빈%오중학
动脉瘤%基质金属蛋白酶%一氧化氮合酶
動脈瘤%基質金屬蛋白酶%一氧化氮閤酶
동맥류%기질금속단백매%일양화담합매
Aneurysm%Matrix metalloproteinases%Nitric oxide synthase
目的 观察基质金属蛋白酶(MMP)2和9及诱导型一氧化氮合酶(iNOS)在脑动脉瘤中的表达,探讨其在脑动脉瘤病理发生中的作用.方法 取2009至2012年内蒙古自治区人民医院及北京天坛医院脑动脉瘤患者经手术切除的标本,共12例作为试验组,取10例颞叶癫痫患者经手术切除的颞叶皮层动脉标本作为对照组.应用明胶酶谱法测定标本MMP2和9的活性,化学比色法测定标本总一氧化氮合酶(TNOS)及iNOS的活性.结果 试验组MMP2和9的比活分别为(199 598±125 288)灰度面积·mg-1·L-1、(719 253±376 519)灰度面积·mg-1·L-1,均明显高于对照组(P＜0.05).试验组TNOS和iNOS活力分别为(23.6±6.6)U/mg蛋白,(11.4±2.6) U/mg蛋白,TNOS试验组与对照组比较差异无统计学意义(P＞0.05),而iNOS的活力与对照组比较差异有统计学意义(P＜0.05),iNOS活力在TNOS活力中所占的比例两组间差异有统计学意义(P＜0.05).结论 MMP2、MMP9、iNOS与脑动脉瘤的形成密切相关.
目的 觀察基質金屬蛋白酶(MMP)2和9及誘導型一氧化氮閤酶(iNOS)在腦動脈瘤中的錶達,探討其在腦動脈瘤病理髮生中的作用.方法 取2009至2012年內矇古自治區人民醫院及北京天罈醫院腦動脈瘤患者經手術切除的標本,共12例作為試驗組,取10例顳葉癲癇患者經手術切除的顳葉皮層動脈標本作為對照組.應用明膠酶譜法測定標本MMP2和9的活性,化學比色法測定標本總一氧化氮閤酶(TNOS)及iNOS的活性.結果 試驗組MMP2和9的比活分彆為(199 598±125 288)灰度麵積·mg-1·L-1、(719 253±376 519)灰度麵積·mg-1·L-1,均明顯高于對照組(P＜0.05).試驗組TNOS和iNOS活力分彆為(23.6±6.6)U/mg蛋白,(11.4±2.6) U/mg蛋白,TNOS試驗組與對照組比較差異無統計學意義(P＞0.05),而iNOS的活力與對照組比較差異有統計學意義(P＜0.05),iNOS活力在TNOS活力中所佔的比例兩組間差異有統計學意義(P＜0.05).結論 MMP2、MMP9、iNOS與腦動脈瘤的形成密切相關.
목적 관찰기질금속단백매(MMP)2화9급유도형일양화담합매(iNOS)재뇌동맥류중적표체,탐토기재뇌동맥류병리발생중적작용.방법 취2009지2012년내몽고자치구인민의원급북경천단의원뇌동맥류환자경수술절제적표본,공12례작위시험조,취10례섭협전간환자경수술절제적섭협피층동맥표본작위대조조.응용명효매보법측정표본MMP2화9적활성,화학비색법측정표본총일양화담합매(TNOS)급iNOS적활성.결과 시험조MMP2화9적비활분별위(199 598±125 288)회도면적·mg-1·L-1、(719 253±376 519)회도면적·mg-1·L-1,균명현고우대조조(P＜0.05).시험조TNOS화iNOS활력분별위(23.6±6.6)U/mg단백,(11.4±2.6) U/mg단백,TNOS시험조여대조조비교차이무통계학의의(P＞0.05),이iNOS적활력여대조조비교차이유통계학의의(P＜0.05),iNOS활력재TNOS활력중소점적비례량조간차이유통계학의의(P＜0.05).결론 MMP2、MMP9、iNOS여뇌동맥류적형성밀절상관.
Objective To explore the expressions of matrix metalloproteinases-2 (MMP-2),matrix metalloproteinases-9 (MMP-9) and inducible nitric oxide synthase (iNOS) in cerebral aneurysms,compare them with normal brain vessels tissue so as to gain a better understanding of the pathogenesis of cerebral aneurysms.Methods Twelve samples of cerebral aneurysms were obtained during operations and 10 cortical arteries as controls during surgery for temporal lobe epilepsy from 2009 to 2012 at Inner Mongolia People's Hospital and Beijing Tiantan Hospital.The activities of MMP-2,MMP-9 and iNOS in specimens were detected with spectrophotometry and substrate gel zymography.Results The MMP-2 and MMP-9 levels in cerebral aneurysm group were (199 598 ± 125 288) gray scale area · mg-1 · L-1 and (719 253 ± 376 519) gray scale area · mg-1 · L-1.Both in cerebral aneurysm group were significantly higher than that in control group(P ＜0.05).The TNOS and iNOS levels in cerebral aneurysm group were(23.6 ± 6.6) and (11.4 ± 2.6) U/mgprot.The difference of TNOS level was not significant between aneurysm and control groups (P ＞0.05) while the levels of iNOS and iNOS/TNOS in cerebral aneurysm group were significantly higher than that in control group (P ＜ 0.05).Conclusion MMP-2,MMP-9 and iNOS are closely correlated with cerebral aneurysm.
