CAJ | 학술논문

目的探讨血清叶酸、同型半胱氨酸(HCY)水平和亚甲基四氢叶酸还原酶(MTHFR)基因多态性与精神分裂症的关系.方法选取2013年1月至2014年1月在郑州大学第一附属医院住院治疗的精神分裂症患者130例(试验组)及同期的80名健康对照者(对照组),采用电化学发光法测血清中叶酸水平,循环酶法测血清中HCY水平,PCR-芯片杂交法测MTHFR C677T基因型,阳性和阴性症状量表(PANSS)评定试验组入组时精神症状.结果 (1)试验组HCY水平[(20±10)μmol/L]高于对照组[(11 ±3) μmol/L] (P ＜0.001),试验组叶酸水平[(6±4) ng/ml]低于对照组[(9±3) ng/ml] (P＜0.001);(2)试验组MTHFR基因C677T位点CC型、CT型、TT型频率分别为13.1％、50.0％、36.9％,对照组的频率分别为30.0％、47.5％、22.5％,两组之间基因型分布差异有统计学意义(x2=36.806,P＜0.05);试验组T、C等位基因频率分别为61.9％、38.1％,对照组T、C等位基因频率分别为46.2％、53.8％,两组间T等位基因频率分布差异有统计学意义(x2=9.872,P＜0.05);试验组TT基因型频率为36.9％,对照组TT基因型频率为22.5％,两组间TT基因纯合型分布差异有统计学意义(x2=4.780,P＜0.05);(3)相关分析显示,试验组叶酸水平和HCY水平呈负相关(r=-0.418,P＜0.001);HCY水平与阴性症状分呈正相关(r=0.345,P＜0.001),叶酸水平与阴性症状分呈负相关(r=-0.386,P＜0.001);(4)在试验组中,MTHFRTT基因型HCY水平明显高于CC基因型、CT基因型(P＜0.05).结论精神分裂症患者血清叶酸、HCY水平与精神分裂症症状之间存在一定相关性,MTHFR基因C677T多态性可能是精神分裂症发病的危险因素之一.
목적 탐토혈청협산、동형반광안산(HCY)수평화아갑기사경협산환원매(MTHFR)기인다태성여정신분렬증적관계.방법 선취2013년1월지2014년1월재정주대학제일부속의원주원치료적정신분렬증환자130례(시험조)급동기적80명건강대조자(대조조),채용전화학발광법측혈청중협산수평,순배매법측혈청중HCY수평,PCR-심편잡교법측MTHFR C677T기인형,양성화음성증상량표(PANSS)평정시험조입조시정신증상.결과 (1)시험조HCY수평[(20±10)μmol/L]고우대조조[(11 ±3) μmol/L] (P ＜0.001),시험조협산수평[(6±4) ng/ml]저우대조조[(9±3) ng/ml] (P＜0.001);(2)시험조MTHFR기인C677T위점CC형、CT형、TT형빈솔분별위13.1％、50.0％、36.9％,대조조적빈솔분별위30.0％、47.5％、22.5％,량조지간기인형분포차이유통계학의의(x2=36.806,P＜0.05);시험조T、C등위기인빈솔분별위61.9％、38.1％,대조조T、C등위기인빈솔분별위46.2％、53.8％,량조간T등위기인빈솔분포차이유통계학의의(x2=9.872,P＜0.05);시험조TT기인형빈솔위36.9％,대조조TT기인형빈솔위22.5％,량조간TT기인순합형분포차이유통계학의의(x2=4.780,P＜0.05);(3)상관분석현시,시험조협산수평화HCY수평정부상관(r=-0.418,P＜0.001);HCY수평여음성증상분정정상관(r=0.345,P＜0.001),협산수평여음성증상분정부상관(r=-0.386,P＜0.001);(4)재시험조중,MTHFRTT기인형HCY수평명현고우CC기인형、CT기인형(P＜0.05).결론 정신분렬증환자혈청협산、HCY수평여정신분렬증증상지간존재일정상관성,MTHFR기인C677T다태성가능시정신분렬증발병적위험인소지일.
Objective To explore the association between serum concentrations of folic acid and homocysteine (HCY),5,10-methylenetetrahydrofolate reductase (MTHFR) C667T polymorphism and schizophrenia.Methods A total of 130 schizophrenics (schizophrenia group) from our hospital and 80 healthy individuals (control group) were enrolled.The serum concentration of homocysteine was measured by the electrochemical luminescence method,the concentration of serum folic acid by enzymatic cycling assay and MTHFR C667T genotype by PCR-DNA microarray.And positive and negative syndrome scale (PANSS) was used to evaluate the mental symptoms.Results (1) Serum HCY level in schizophrenia group was higher than that in control group [(20 ± 10) vs (11 ± 3) μmol/L; P ＜ 0.001] while serum folate level in schizophrenia group was lower than that in control group [(6 ±4) vs (9 ±3) ng/ml; P ＜0.001] ; (2)the frequencies of CC,CT and TT in MTHFR C677T alleles were 13.1％,50.0％ and 36.9％ in schizophrenia group versus 30.0％,47.5％ and 22.5％ in control group respectively.Statistical significant inter-group difference existed in the frequencies of genotypes (x2 =36.806,P ＜ 0.05).The frequencies of T and C alleles were 61.9％,38.1％ in schizophrenia group versus 46.25％,53.75％ in control group respectively.Statistical significant inter-group difference existed in the frequency of T allele (x2 =9.872,P＜0.05).The frequency of TT genotype in schizophrenia group was 61.9％ versus 22.5％ in control group.Statistically significant inter-group difference existed in the frequency of TT homozygous mutation (x2 =4.780,P ＜ 0.05) ; (3) correlation analysis showed that serum folate level in schizophrenia group had a negative correlation with HCY level (r =-0.418,P ＜ 0.001).Serum HCY level had a positive correlation with negative symptoms scores (r =0.345,P ＜ 0.001) and serum folate level was negatively correlated with negative symptoms scores (r =-0.386,P ＜ 0.001) ; (4) the serum HCY level in schizophrenia group with TT genotype was significantly higher than those with CC and CT genotypes (P ＜0.05).Conclusion There is a certain correlation between the serum levels of folate and HCY and the symptoms of schizophrenia.And MTHFR C677T polymorphism is a possible risk factor for schizophrenia.