CAJ | 학술논문

目的探讨流式细胞学技术对非霍奇金淋巴瘤(NHL)骨髓侵犯的诊断价值.方法连续选取2013年3至8月在北京大学肿瘤医院淋巴肿瘤科诊断的NHL 206例患者同时进行骨髓涂片、骨髓活检以及骨髓流式细胞学检测,对结果进行回顾性分析和比较.结果本组NHL病例的骨髓侵犯比例为25.7％(53/206).其中14例T淋巴细胞母细胞性淋巴瘤/急性淋巴细胞白血病(T-LBL/ALL)患者其骨髓涂片、活检及流式细胞学检测阳性率分别为6/14、6/14和9/14;152例成熟B细胞NHL患者中骨髓涂片、活检及流式细胞学检测的阳性率分别为9.2％ (14/152)、19.1％(29/152)和23.7％ (36/152);40例成熟T/NK细胞淋巴瘤患者中骨髓涂片、活检及流式细胞学检测的阳性率分别是0(0/40)、15.0％ (6/40)和2.5％(1/40).骨髓流式细胞学检测和骨髓活检的诊断一致性高达88.3％.结论骨髓流式细胞学检测对于诊断NHL,尤其T-LBL/ALL和B细胞NHL骨髓侵犯的敏感性高,和骨髓活检的诊断一致性好.通过对骨髓涂片、活检及流式细胞学检测结果的综合分析,可提高NHL骨髓侵犯的诊断的敏感性和准确性.
목적 탐토류식세포학기술대비곽기금림파류(NHL)골수침범적진단개치.방법 련속선취2013년3지8월재북경대학종류의원림파종류과진단적NHL 206례환자동시진행골수도편、골수활검이급골수류식세포학검측,대결과진행회고성분석화비교.결과 본조NHL병례적골수침범비례위25.7％(53/206).기중14례T림파세포모세포성림파류/급성림파세포백혈병(T-LBL/ALL)환자기골수도편、활검급류식세포학검측양성솔분별위6/14、6/14화9/14;152례성숙B세포NHL환자중골수도편、활검급류식세포학검측적양성솔분별위9.2％ (14/152)、19.1％(29/152)화23.7％ (36/152);40례성숙T/NK세포림파류환자중골수도편、활검급류식세포학검측적양성솔분별시0(0/40)、15.0％ (6/40)화2.5％(1/40).골수류식세포학검측화골수활검적진단일치성고체88.3％.결론 골수류식세포학검측대우진단NHL,우기T-LBL/ALL화B세포NHL골수침범적민감성고,화골수활검적진단일치성호.통과대골수도편、활검급류식세포학검측결과적종합분석,가제고NHL골수침범적진단적민감성화준학성.
Objective To evaluate the value of flow cytometry (FCM) in the diagnosis of bone marrow involvement in patients with non-Hodgkin's lymphoma (NHL).Methods The cytomorphology,biopsy and FCM tests were performed concurrently on bone marrow samples from 206 patients who were consecutively diagnosed as NHL from March 2013 to August 2013 at Peking University Cancer Hospital.The results were analyzed and compared.Results Totally bone marrow involvement occurred in 25.7％ (53/206) patients.The detection positivity of bone marrow involvement by cytomorphology,biopsy and flow cytometry were 6/14,6/14 and 9/14 respectively in 14 T-cell lymphoblastic lymphoma and T-cell acute lymphoblastic leukemia (T-LBL/ALL) patients ; 9.2％ (14/152),19.1％ (29/152) and 23.7％ (36/152)respectively in 152 mature B-cell NHL patients; 0(0/40),15.0％ (6/40) and 2.5％ (1/40)in 40 cases of mature T/NK-cell NHL.The overall rate of concordance of biopsy and flow cytometry was 88.3％.Conclusions FCM has a high sensitivity in detecting bone marrow involvement in NHL patients,especially those with T-LBL/ALL and B-cell NHL.And a combination of morphology,biopsy and FCM may improve the sensitivity and accuracy in the detection of bone marrow involvement in NHL patients.